2018
DOI: 10.1590/1678-4162-9684 View full text |Buy / Rent full text
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Abstract: The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye… Show more

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“…Recently, clear evidence has been presented that this potent antioxidant specifically acts by enhancing OXPHOS activity both at baseline and, to a greater extent, at capacitation in vitro [ 54 ]. In a study applying different concentrations of GSH (5, 7.5 and 10 mM), it was possible to observe high mitochondrial activity in refrigerated or thawed semen samples at a dose of 5 mM GSH [ 73 ]. The reducing stress effect of high doses of inositol on mitochondria causes an increase in reactive oxygen species and mitochondrial dysfunction, resulting in dysfunctions in ATP synthesis [ 74 ].…”
Section: Discussionmentioning
“…The reducing stress effect of high doses of inositol on mitochondria causes an increase in reactive oxygen species and mitochondrial dysfunction, resulting in dysfunctions in ATP synthesis [ 74 ]. Thus, it can be stated that high doses of inositol do not cause a stress effect [ 73 ]. Low-intensity oxidative stress can be helpful to cells, whereas high levels can bring about degradation of nucleic acids, proteins, lipids and carbohydrates, ultimately leading to cell death [ 38 , 43 , 44 ].…”
Section: Discussionmentioning