“…The addition of antibiotics to control bacteria in sperm samples is routinely used in humans, livestock, and aquaculture species [ 96 , 97 ]. In fish, penicillin, streptomycin, erythromycin, gentamicin, and neomycin are the most commonly used antibiotics [ 72 , 77 ]. The addition of antibiotics to fish semen has been reported to improve sperm quality (motility, velocity and viability) and longevity during short-term storage in species including common carp ( C. carpio ) [ 91 ], Atlantic cod ( Gadus morhua ), haddock ( Melanogrammus aeglefinus ) [ 98 ], Atlantic salmon ( S. salar ) [ 90 ], African catfish ( Clarias gariepinus ) [ 92 ], Caspian brown trout ( Salmo trutta caspius ) [ 99 ], Nile tilapia ( Oreochromis niloticus ) [ 96 ], spotted halibut ( Verasper variegatus ) [ 77 ], and other commercially valuable fish species [ 72 ].…”