The Use of Immobilized Templates—A New Approach in Molecular Imprinting

Abstract

By using porous silica gel as a solid support, template molecules can be immobilized to give a novel approach to molecular imprinting in polymers. Polymerizable, functional monomers assemble around a template (such as theophylline, see picture). Following polymerization, this support is sacrificed by dissolving in HF. The remaining polymer shows a specific binding capacity for theophylline, but not for related compounds, such as theobromine and caffeine. All binding sites are uniformly oriented and located at the surface of the polymer.

The rational development of molecularly imprinted polymer-based sensors for protein detection

“…The imprints were shown to be capable of binding larger peptides with the same terminal sequence as the template peptide (epitope). 130 This method is a development of that shown for small molecules 131,132 and is also similar in concept to the nanowire method, shown in Figure 8.…”

Polímeros biomiméticos em química analítica. Parte 1: preparo e aplicações de MIP ("Molecularly Imprinted Polymers") em técnicas de extração e separação

“…Após o término da reação, o suporte sólido e o analito são removidos por dissolução química com solução de HF criando, desta maneira, os sítios seletivos 68 . A dissolução química é realizada com HF, pois se trata de um ácido "polimérico", cuja função é apenas dissolver a sílica preservando a matriz polimérica.…”

Molecularly Imprinted Materials: Towards the Next Generation

“…An alternative route demonstrated by our group and others was to use a template immobilized on a solid support [17]. Following imprinting polymerization, the template and the carrier support were removed by chemical dissolution, which leaves surface imprinted sites on the obtained MIP.…”