The rational development of molecularly imprinted polymer-based sensors for protein detection

Whitcombe, Michael J.; Chianella, Iva; Larcombe, Lee; Piletsky, Sergey A.; Noble, James; Porter, Robert A.; Horgan, Adrian

doi:10.1039/c0cs00049c

Cited by 649 publications

(403 citation statements)

References 275 publications

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“… HPLC on MIP columns [39,[44][45][46]  Solid phase extraction (SPE or MISPE) [47,48]  Membrane separations [49][50][51]  Sensors [52][53][54]  Binding assays [55][56][57]  Selective removal of low concentration contaminants in industrial or environmental technologies [5,8,9] In all of these applications selective separation or detection are needed. If selectivity between widely different compounds or groups of compounds is required, MIPs are suitable for any of the above tasks.…”

Section: Using the Log-log Isotherms To Understand And Design Practicmentioning

confidence: 99%

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Dorkó

Tamás

Horvai

2017

Talanta

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A simple and efficient method is presented for assessing molecularly imprinted polymers (MIP) and other sorbents from the point of view of practical applications. The adsorption isotherms of the compounds, which need to be separated or detected in an application, are constructed from a small number of measured points on a log-log chart and then are compared graphically. Despite its simplicity and robustness this method reveals the information needed for optimal selection between MIPs and alternative sorbents. The design of separation or detection methods with MIPs is also supported by the proposed graphical isotherm comparison. Many experimental isotherms are presented supporting the proposed method.

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Section: Using the Log-log Isotherms To Understand And Design Practicmentioning

confidence: 99%

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Dorkó

Tamás

Horvai

2017

Talanta

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“…11,12 MIPs have been made with various types of monomers, mostly of which are acrylic and silane based. 13,14 However, most MIPs suffer from poor binding affinity or specificity. 15,16 At the same time, it is difficult to produce a fluorescence signal for binding.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Zhang

Liu

2016

ACS Appl. Mater. Interfaces

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Molecularly imprinted polymers (MIPs) are produced in the presence of a template molecule. After removing the template, the cavity can selectively rebind the template. MIPs are attractive functional materials with a low cost and high stability, but traditional MIPs often suffer from low binding affinity. This study employs DNA aptamer fragments as macromonomers to improve MIPs. The DNA aptamer for adenosine was first split into two halves, fluorescently labeled, and co-polymerized into MIPs. With a fluorescence quenching assay, the importance of imprinting was confirmed. Further studies were carried out using isothermal titration calorimetry (ITC).Compared to the mixture of the free aptamer fragments, their MIPs doubled the binding affinity.Each free aptamer fragment alone cannot bind adenosine, while MIPs containing each fragment are effective binders. We further shortened one of the aptamer fragments, and the DNA length was pushed to as short as six nucleotides, yielding MIPs with a dissociation constant of 27 µM adenosine. This study provides a new method for preparing functional MIP materials by combining high affinity biopolymer fragments with low cost synthetic monomers, allowing higher binding affinity and providing a method for signaling binding based on DNA chemistry.

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“…Few strategies of protein surface imprinting in cardiac biomarker sensing context have been employed so far [23][24][25][26][27][28][29], involving surface imprinting procedures on gold [24,[28][29][30], silica [25,26], or carbon [27], while employing different approaches for assembling the polymeric network. Among these, electropolymerization seems to be the simplest approach towards protein imprinting that seems to have been developed first by Panasyuk et al for a small target analyte [31], and applied later to proteins by Cai et al [32].…”

Section: Introductionmentioning

confidence: 99%

“…While the imprinting of small molecules has been successfully achieved, protein imprinting is still a challenge [23]. The restricted protein mobility within the polymeric network and the weak efficiency/reversibility of binding are major technical hitches.…”

Section: Introductionmentioning

confidence: 99%

Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

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This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon-sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing prot ein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate-rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The d evices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 µg/mL, respectively, with detection limits of 1.5 and 0.8 µg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivit y and selectivity.

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The rational development of molecularly imprinted polymer-based sensors for protein detection

Cited by 649 publications

References 275 publications

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Isotherm charts for material selection and method development with molecularly imprinted polymers and other sorbents

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Protein-responsive polymers for point-of-care detection of cardiac biomarker

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