Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Zhang, Zijie; Liu, Juewen

doi:10.1021/acsami.6b00461

Cited by 71 publications

(45 citation statements)

References 51 publications

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“…In one strategy, the biomolecule is acrylated and included during the polymerization, 10 which works well for biomolecules that are soluble and stable in the reaction medium. Alternatively, postsynthetic modifications can decorate hydrogels with recognitive biomolecules.…”

Section: Classes Of Analyte-sensitive Materialsmentioning

confidence: 99%

Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery

2017

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CONSPECTUS: Nature has mastered the art of molecular recognition. For example, using synergistic non-covalent interactions, proteins can distinguish between molecules and bind a partner with incredible affinity and specificity. Scientists have developed, and continue to develop, techniques to investigate and better understand molecular recognition. As a consequence, analyte-responsive hydrogels that mimic these recognitive processes have emerged as a class of intelligent materials. These materials are unique not only in the type of analyte to which they respond but also in how molecular recognition is achieved and how the hydrogel responds to the analyte. Traditional intelligent hydrogels can respond to environmental cues such as pH, temperature, and ionic strength. The functional monomers used to make these hydrogels can be varied to achieve responsive behavior. For analyte-responsive hydrogels, molecular recognition can also be achieved by incorporating biomolecules with inherent molecular recognition properties (e.g., nucleic acids, peptides, enzymes, etc.) into the polymer network. Furthermore, in addition to typical swelling/syneresis responses, these materials exhibit unique responsive behaviors, such as gel assembly or disassembly, upon interaction with the target analyte. With the diverse tools available for molecular recognition and the ability to generate unique responsive behaviors, analyte-responsive hydrogels have found great utility in a wide range of applications. In this Account, we discuss strategies for making four different classes of analyte-responsive hydrogels, specifically, nonimprinted, molecularly imprinted, biomolecule-containing, and enzymatically responsive hydrogels. Then we explore how these materials have been incorporated into sensors and drug delivery systems, highlighting examples that demonstrate the versatility of these materials. For example, in addition to the molecular recognition properties of analyte-responsive hydrogels, the physicochemical changes that are induced upon analyte binding can be exploited to generate a detectable signal for sensing applications. As research in this area has grown, a number of creative approaches for improving the selectivity and sensitivity (i.e., detection limit) of these sensors have emerged. For applications in drug delivery systems, therapeutic release can be triggered by competitive molecular interactions or physicochemical changes in the network. Additionally, including degradable units within the network can enable sustained and responsive therapeutic release. Several exciting examples exploiting the analyte-responsive behavior of hydrogels for the treatment of cancer, diabetes, and irritable bowel syndrome are discussed in detail. We expect that creative and combinatorial approaches used in the design of analyte-responsive hydrogels will continue to yield materials with great potential in the fields of sensing and drug delivery.

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Section: Classes Of Analyte-sensitive Materialsmentioning

confidence: 99%

Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery

2017

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“…This adenosine aptamer contains 27 nucleotides and can specifically bind two adenosine molecules ( Figure A) . We tested the idea of using split aptamer in MIPs in 2016 . This adenosine aptamer was split to two halves and each fragment was modified with an acrydite (Figure B, named F1 and F2).…”

Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

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Molecular Imprinting with Functional DNA

Zhang

Liu

2019

Small

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Molecular imprinting refers to templated polymerization with rationally designed monomers, and this is a general method to prepare stable and cost‐effective ligands. This attractive concept however suffers from low affinity, low specificity, and limited signaling mechanisms for binding. Acrydite‐modified DNA oligonucleotides can be readily copolymerized into acrylic polymers. With molecular recognition and catalytic functions, such functional DNAs are recently shown to enhance the performance of molecularly imprinted polymers (MIPs) in a few ways. First, DNA aptamers are used as macromonomers to enhance binding affinity and specificity of MIPs. Second, DNA can help produce optical signals to follow binding events. Third, imprinting can also improve the performance of catalytic DNA by enhancing its activity and specificity toward the template substrate. Finally, MIP is shown to help aptamer selection. Bulk imprinting, nanoparticle imprinting, and surface imprinting are all demonstrated with DNA. Since both DNA and synthetic polymers are cost effective and stable, their hybrid materials still possess such properties while enhancing the function of each component. This review covers recent developments on the abovementioned aspects of DNA‐containing MIPs, a field just emerged in the last five years, and future research directions are discussed toward the end.

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“…Why not integrate a peptide binding motif in a synthetic MIP scaffold and use it for harsh conditions which is not the domain of natural antibodies? Most recently, first attempts on hybrid MIP-DNA aptamer coatings have been reported and it will be interesting to learn if the MIP sensor community will be successful in applying such adopted receptor strategies in the future [28,29]. …”

Section: Random or Pre-defined Polymeric Sequence For Synthetic Bimentioning

confidence: 99%

One Binder to Bind Them All

Hayden

2016

Sensors

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High quality binders, such as antibodies, are of critical importance for chemical sensing applications. With synthetic alternatives, such as molecularly imprinted polymers (MIPs), less sensor development time and higher stability of the binder can be achieved. In this feature paper, I will discuss the impact of synthetic binders from an industrial perspective and I will challenge the molecular imprinting community on the next step to leapfrog the current status quo of MIPs for (bio)sensing. Equally important, but often neglected as an effective chemical sensor, is a good match of transducer and MIP coating for a respective application. To demonstrate an application-driven development, a biosensing use case with surface-imprinted layers on piezoacoustic sensors is reported. Depending on the electrode pattern for the transducer, the strong mechanical coupling of the analyte with the MIP layer coated device allows the adoption of the sensitivity from cell mass to cell viability with complete reversibility.

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Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Cited by 71 publications

References 51 publications

Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery

Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery

Molecular Imprinting with Functional DNA

One Binder to Bind Them All

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