The use of hyaluronan and its sulphated derivative patterned with micrometric scale on glass substrate in melanocyte cell behaviour

Polyelectrolyte Multilayer Films as Substrates for Photoreceptor Cells

“…The glass slides coated with polyelectrolyte films only were visualized as smooth surfaces by SEM (Figure 10a), whereas presence of the insoluble fraction of IPM was seen in the form of fibrils ( Figure 10b). 56,74 Photoreceptor cells on IPM absorbed polyelectrolyte films are shown in Figure 10c. When cell numbers were scored after 2 days in vitro, there were greater numbers of immunolabeled cones in the presence of free bFGF and IPM absorbed on top (p<0.05) than on (PLL/CSA) 10 /PLL multilayers alone (Figure 11a, white bars).…”

Synthesis of photoreactive pullulan for surface modification

“…Barbucci et al [18] synthesized photoreactive hyaluronan and its sulfated derivative for micropattern immobilization. We have modified growth factors [19], sulfated hyaluronic acid [20], heparin [21], thermo-responsive polymers [22], and b-galactose derivatives [23], and photoimmobilized them on various surfaces using the method devised by Matsuda and Sugawara [24].…”

Novel Poly(amido‐amine)‐Based Hydrogels as Scaffolds for Tissue Engineering

“…Hydrogels are biomimetic materials with a reckoned potential as scaffolds for tissue engineering applications, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] which derives from relevant properties, such as tissue-like water content and permeability to oxygen and metabolites, combined with ease in implantation (in situ forming) and in encapsulation of cells and signalling factors (e. g. cell growth factors) during crosslinking. The primary function of a scaffold is to facilitate cell attachment, multiplication, and differentiation, i. e., transformation from a non-specific or primitive state into cells exhibiting tissue-specific functions, eventually organising into a normal, healthy tissue, while the scaffold degrades.…”