The use of comet assay data with a simple reaction mechanism to evaluate the relative effectiveness of free radical scavenging by quercetin, epigallocatechin gallate and N-acetylcysteine in UV-irradiated MRC5 lung fibroblasts

Salter, Leo; Clifford, Tim; Morley, Nick; Gould, David; Campbell, Sandra J.; Curnow, Alison

doi:10.1016/j.jphotobiol.2004.05.007

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…Invivo experimentalstudieshaveconfirmed the protectivee ffectof erdosteine against the free radicalinduced toxicity of compoundss uchasparaquat,c hemotherapeutica gents suchascisplatin and doxorubicin,paracetamol,vancomycin and isoniazid [33].The anti-oxidantactivity of MET Ihasbeen demonstrated underdifferentexperimentalconditionsu sing PMA as stimulantin neutrophilsand eosinophils [12].The previous studiesareconsistentwiththe antioxidantprotectivee ffectof MetI;however,no dataa bout the possible protection against oxidativeDNA damage havebeen yet collected. Our findingsarei nagreementw ithp revious findingsand showt hatMET Ihasalsoani mportant concentration-dependentactivity in reducing the H 2 O 2 -induced DNA damage,and thisgenoprotectiveeffectisin line withthe similarlyprotectivee ffectof the anothert hiol molecule,the N-acetylcysteine [34][35][36]. The ability of thiolst op rotectcellsisgenerallyattributed totheirfree radicalscavenging activity protecting against GSH oxidation,b ut att he same time,theyarea sourceo fcysteine fort he synthesisof GSH itself.…”

Section: Discussionsupporting

confidence: 70%

Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells

Marabini

Calò

Braga

2012

Arzneimittelforschung

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It has been shown that the mucolytic agent erdosteine (N-carboxymethylthio-acetyl-homocysteine thiolactone, CAS 84611-23-4) has anti-inflammatory and anti-oxidant properties, and an active metabolite I (MET I) containing pharmacologically active sulphydryl group has been found to have a free radical scavenging activity. The aim of this study was to assess the ability of erdosteine metabolite I to protect A549 human lung adenocarcinoma cell against hydrogen peroxide (H2O2)-mediated oxidative stress and oxidative DNA damage. When A549 cells were pre-treated with the active metabolite I (2.5-5-10 microg/ml) for 10-30 min and then exposed to H2O2 (1-4 mM) for two additional hours at 37 degrees C, 5% at CO2, the intracellular peroxide production, reflected by dichlorofluorescein (DCF) fluorescence, decreased in a concentration-dependent manner. Furthermore, using a comet assay as an indicator for oxidative DNA damage, it was found that the metabolite I prevented damage to cells exposed to shortterm H2O2 treatment. The data suggest that this compound is effective in preventing H2O2-induced oxidative stress and DNA damage in A549 cells. The underlying mechanisms involve the scavenging of intracellular reactive oxygen species (ROS).

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Section: Discussionsupporting

confidence: 70%

Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells

Marabini

Calò

Braga

2012

Arzneimittelforschung

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“…EGCG has multiple effects that can vary with the system of study, but that usually are considered anti-inflammatory, such as via inhibition of NF-κB and other pathways (33,34), and typically anti-oxidant, although pro-oxidant effects have been described at high concentrations (35). With the goal of defining potential mechanisms by which EGCG is anti-inflammatory, we have shown here that EGCG markedly decreases antigen-specific airway inflammation in a murine Th1 cell adoptive transfer model.…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-Gallate Reduces Airway Inflammation in Mice through Binding to Proinflammatory Chemokines and Inhibiting Inflammatory Cell Recruitment

Qin

Alcorn

Craigo

et al. 2011

The Journal of Immunology

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One major activity of chemokines is recruiting immune cells to sites of infection and inflammation. CD4+ Th1 cells play critical roles in host defense against pathogens and in the pathogenesis of many immune-mediated diseases. It has been reported that epigallocatechin-3-gallate (EGCG) exhibits anti-inflammatory properties, but the mechanisms have not been completely defined. In this study, we found that EGCG markedly decreased recruitment of murine ovalbumin (OVA)-specific Th1 cells and other inflammatory cells into the airways in a Th1 adoptive transfer mouse model. In vitro analysis revealed that EGCG inhibits CXCR3 ligand driven chemotaxis of murine and human cells. Surface plasmon resonance studies revealed that EGCG bound directly to chemokines CXCL9, CXCL10 and CXCL11. These results indicate that one anti-inflammatory mechanism of EGCG is binding of proinflammatory chemokines and limiting their biological activities. These findings support further development of EGCG as a potent therapeutic for inflammatory diseases.

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“…Quercetin as other flavonoids are able to protect against chemically induced DNA damage in human lymphocytes and increase the total antioxidant capacity of plasma (Wilms et al, 2005; Tieppo et al, 2007), increased genomic stability in cirrhotic rats, suggesting beneficial effects, probably by its antioxidant properties. Flavonoids can also protect cells by acting as free radical scavengers, inhibiting DNA damage and mutagenicity (Edenharder and Grü nhage, 2003;Salter et al, 2004). Otherwise, the hydroxycinnamic acid derivatives, such as 5-caffeoylquinic acid and caffeic acid present an antioxidant activity upon low density lipoprotein peroxidation (Laranjinha et al, 1994).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars

Pereira

Oliveira

Sousa

et al. 2007

Food and Chemical Toxicology

371

225

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Different cultivars of walnut (Juglans regia L.) leaves (Cv. Lara, Franquette, Mayette, Marbot, Mellanaise and Parisienne) grown in Portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. Phenolics analysis was performed by reversed-phase HPLC/DAD and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, quercetin 3-arabinoside, quercetin 3-xyloside and quercetin 3-rhamnoside. The antimicrobial capacity was screened against Gram positive (Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans). Walnut leaves selectively inhibited the growth of Gram positive bacteria, being B. cereus the most susceptible one (MIC 0.1mg/mL). Gram negative bacteria and fungi were resistant to the extracts at 100mg/mL. Lara walnut leaves were also submitted to antibacterial assays using 18 clinical isolates of Staphylococcus sp. Antioxidant activity was accessed by the reducing power assay, the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and beta-carotene linoleate model system. In a general way, all of the studied walnut leaves cultivars presented high antioxidant activity (EC(50) values lower than 1mg/mL), being Cv. Lara the most effective one.

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The use of comet assay data with a simple reaction mechanism to evaluate the relative effectiveness of free radical scavenging by quercetin, epigallocatechin gallate and N-acetylcysteine in UV-irradiated MRC5 lung fibroblasts

Cited by 8 publications

References 6 publications

Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells

Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells

Epigallocatechin-3-Gallate Reduces Airway Inflammation in Mice through Binding to Proinflammatory Chemokines and Inhibiting Inflammatory Cell Recruitment

Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars

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