Epigallocatechin-3-Gallate Reduces Airway Inflammation in Mice through Binding to Proinflammatory Chemokines and Inhibiting Inflammatory Cell Recruitment

Qin, Shulin; Alcorn, John F.; Craigo, Jodi K.; Tjoeng, Charis; Tarwater, Patrick M.; Kolls, Jay K.; Reinhart, Todd A.

doi:10.4049/jimmunol.1002876

Cited by 40 publications

(34 citation statements)

References 44 publications

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“…A new antinflammatory mechanism of EGCG based on its binding activity to chemokines has been reported [137]. Surface plasmon resonance studies have demonstrated that EGCG directly binds to CXCL9, CXCL10 and CXCL11, thus hampering their inflammatory activities in a murine model of airway inflammation.…”

Section: Resultsmentioning

confidence: 99%

Immunopathogenesis of Neurodegenerative Diseases: Current Therapeutic Models of Neuroprotection with Special Reference to Natural Products

Magrone¹,

Marzulli²,

Jirillo

2012

CPD

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Parkinson disease (PD) and Alzheimer disease (AD) are neurodegenerative processes whose frequency is dramatically increasing in the western world. Both diseases share a common pathogenic denominator characterized by an exaggerated activation of the systemic and cerebral immune system, respectively. For instance, lipopolysaccharides in PD and amyloid beta in AD trigger microglia and astrocytes to release reactive oxygen species (ROS) and proinflammatory cytokines. Infiltrating peripheral T cells once activated in the central nervous system also contribute to the neurodegenerative process. Besides innovative biotherapy, nutraceuticals or functional foods are currently investigated for their neuroprotective activities. Especially, vitamin D and polyphenols, seem to be promising therapeutic tools for inhibiting ROS formation and arresting cytokine-mediated neuroinflammation in PD and AD.

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Section: Resultsmentioning

confidence: 99%

Immunopathogenesis of Neurodegenerative Diseases: Current Therapeutic Models of Neuroprotection with Special Reference to Natural Products

Magrone¹,

Marzulli²,

Jirillo

2012

CPD

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“…Transfections were performed as described previously [18, 19]. Briefly, the L1.2 murine pre-B cell line [18–20] was electroporated with pcDNA3.1(+)-fCCR6.1, and stably transfected cells were obtained after two rounds of single cell cloning in the presence of 1mg/ml G418 (Life Technologies).…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of ferret CCL20 and CCR6 and identification of chemotactic inhibitors

et al. 2013

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CCL20 is currently the only known chemokine ligand for the receptor CCR6, and is a mucosal chemokine involved in normal and pathological immune responses. Although nucleotide sequence data are available for ccl20 and ccr6 sequences from multiple species, the ferret ccl20 and ccr6 sequences have not been determined. To increase our understanding of immune function in ferret models of infection and vaccination, we have used RT-PCR to obtain the ferret ccl20 and ccr6 cDNA sequences and functionally characterize the encoded proteins. The open reading frames of both genes were highly conserved across species and mostly closely related to canine sequences. For functional analyses, single cell clones expressing ferret CCR6 were generated, a ferret CCL20/mouse IgG2a fusion protein (fCCL20-mIgG2a) was produced, and fCCL20 was chemically synthesized. Cell clones expressing ferret CCR6 responded chemotactically to fCCL20-mIgG2a fusion protein and synthetic ferret CCL20. Chemotaxis inhibition studies identified the polyphenol epigallocatechin-3-gallate and the murine γ-herpesvirus 68 M3 protein as inhibitors of fCCL20. Surface plasmon resonance studies revealed that EGCG bound directly to fCCL20. These results provide molecular characterization of previously unreported ferret immune gene sequences and for the first time identify a broad-spectrum small molecule inhibitor of CCL20 and reveal CCL20 as a target for the herpesviral M3 protein.

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“…Literature reports indicate that the effect of GTE on the immune system depends on the dose and can either suppress or enhance peripheral blood mononuclear cell proliferation (Qin et al, 2011), (Kim, Quon, Kim, 2014). The effectiveness of GTE supplementation may be related to individual characteristics, age, gender or physical fitness level.…”

Section: Discussionmentioning

confidence: 99%

Effect of Long-Term Green Tea Extract Supplementation on Peripheral Blood Leukocytes in CrossFit-Trained and Untrained Men

Żak

Pokora

2017

Central European Journal of Sport Sciences and Medicine

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The aim of the study was to evaluate the effects of long-term green tea extract (GTE) supplementation on white blood cells (WBC) in CrossFit-trained and untrained men. Twenty-eight men were recruited for this study including CrossFit-trained (CF-TR) and untrained (CF-UNT) men. The study was carried out in two phases. Phase 1 involved non-supplemented untrained and trained men. Phase 2 involved the same groups but after 6-week supplementation with 2 GTE capsules once daily. Body weight, body composition and the number and percentage of peripheral blood leukocyte populations were assessed in each phase. The results revealed that the long-term green tea supplementation did not have a significant effect on body weight, body composition and the total white blood cell count of the study participants. However, the percentage of peripheral blood eosinophils increased while monocyte and neutrophil counts decreased. The changes in white blood cells were less pronounced in CrossFit-trained compared to control participants. Summing up, it should be noted that long-term GTE supplementation in CrossFit-trained men did not result in significant changes in body weight and composition and the total white blood cell count. However, the changes in leukocyte subsets were less pronounced in CrossFit-trained compared to untrained participants.

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Epigallocatechin-3-Gallate Reduces Airway Inflammation in Mice through Binding to Proinflammatory Chemokines and Inhibiting Inflammatory Cell Recruitment

Cited by 40 publications

References 44 publications

Immunopathogenesis of Neurodegenerative Diseases: Current Therapeutic Models of Neuroprotection with Special Reference to Natural Products

Immunopathogenesis of Neurodegenerative Diseases: Current Therapeutic Models of Neuroprotection with Special Reference to Natural Products

Functional characterization of ferret CCL20 and CCR6 and identification of chemotactic inhibitors

Effect of Long-Term Green Tea Extract Supplementation on Peripheral Blood Leukocytes in CrossFit-Trained and Untrained Men

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