In this work, combustion process of diesel and biofuel blends is studied experimentally in an isothermal oven. Blends with respectively 20%, 40% and 60% of Ethanol (E20, E40, E60), 20%, 40% and 60% of Methanol (M20, M40, M60), and 20%, 40%, 60%, 80% and 100% of Biodiesel (B20, B40, B60, B80, B100), are comparatively analyzed focusing on wall temperature of the isothermal oven and emissions products from combustion. It is found that wall temperature drops with biodiesel content in blends and is higher compared with pure diesel, whatever the content of biodiesel. For diesel-ethanol and diesel-methanol blends the wall temperature is less than pure diesel. Unburned HCs and NOX emissions are very low for all blends and CO emissions increase with higher ethanol and methanol blends. For industrial applications that need high temperature (as drying, cooking, sterilization, others) the combustion of diesel-biodiesel blends are recommended.