The use of antinucleoside antibodies to probe the organization of chromosomes denatured by ultraviolet irradiation

Schreck, R.R.; Erlanger, Bernard F.; Miller, Orlando J.

doi:10.1016/0014-4827(74)90614-4

Cited by 60 publications

(24 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the banding with anti-A or anti-T is similar to that obtained with guanine or giemsha and is the reverse of that obtained with anti-C. Anti-5methylcytosine stained most intensely the centromere regions of human chromosome 1,9, 15, 6 16 and Y [57,58] . Results using the immunoperoxidase technique [57] were identical to those obtained by immunofluorescence [58] but the former has several technical advantages. It has been suggested that the banding patterns represent the organization of large amounts of repetitive DNA into distinct bands [56].…”

Section: Chromosome Structuresupporting

confidence: 63%

See 1 more Smart Citation

Chromatin structure and specificity revealed by immunological techniques

Bustin

1976

FEBS Letters

View full text Add to dashboard Cite

show abstract

Section: Chromosome Structuresupporting

confidence: 63%

“…DNA Antibodies specific to each A, T, C, G, and 5 methyl-C [55,59], when reacting with chromosomes denatured by treatment with formamide [55], or exposed to ultraviolet [57,58] or photoxidized in presence of methylene blue [56] give specific banding patterns. The antibodies react only with single stranded regions.…”

Section: Chromosome Structurementioning

confidence: 99%

Chromatin structure and specificity revealed by immunological techniques

Bustin

1976

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Miller to the chromosomes of several mammalian species, including human, chimpanzee, gorilla, cattle, mouse, and kangaroo rat Schreck et al, 1974Schreck et al, , 1977Schnedl et al, 1975Schnedl et al, , 1976. Using an immunofluorescence technique and anti-5-MeC antibodies, they showed that methylated DNA can be detected in fixed metaphase chromosomes after they have been UV-irradiated to generate regions of single-stranded DNA.…”

mentioning

confidence: 99%

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

Schmid

Steinlein

Yano

et al. 2015

Cytogenet Genome Res

View full text Add to dashboard Cite

Sites and amounts of 5-methylcytosine (5-MeC)-rich chromosome regions were detected in the karyotypes of 9 Brazilian species of Characiformes fishes by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. These species, belonging to the genera Leporinus, Triportheus and Hoplias, are characterized by highly differentiated and heteromorphic ZW and XY sex chromosomes. In all species, the hypermethylated regions are confined to constitutive heterochromatin. The number and chromosome locations of hypermethylated heterochromatic regions in the karyotypes are constant and species-specific. Generally, heterochromatic regions that are darkly stained by the C-banding technique are distinctly hypermethylated, but several of the brightly fluorescing hypermethylated regions merely exhibit moderate or faint C-banding. The ZW and XY sex chromosomes of all 9 analyzed species also show species-specific heterochromatin hypermethylation patterns. The analysis of 5-MeC-rich chromosome regions contributes valuable data for comparative cytogenetics of closely related species and highlights the dynamic process of differentiation operating in the repetitive DNA fraction of sex chromosomes.

show abstract

“…inactive (25) and silver staining (26) were performed as described. The binding of anti-5-methylcytidine antibodies to denatured chromosomal DNA was detected by an indirect immunoperoxidase technique (27) after denaturing the DNA by UV irradiation (28) or photooxidation (29).…”

mentioning

confidence: 99%

Amplified ribosomal RNA genes in a rat hepatoma cell line are enriched in 5-methylcytosine.

Tantravahi¹,

Guntaka²,

Erlanger³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In a rat hepatoma cell line, H4-IIE-C3, a 10-fold excess of 18S and 28S rRNA genes has been found in amplified chromosome regions. Antibodies to 5-methylcytidine bound extensively to the DNA of these regions, indicating a high level of DNA methylation. Most of the amplified rRNA genes were transcriptionally inactive, as shown by their failure to stain with silver. DNAs from the tumor cells and control rat hepatocytes grown with L-[methyl-'4C]methionine were digested with restriction endonuclease EcoRI; the DNA fragments were separated by agarose gel electrophoresis, denatured, transferred to nitrocellulose filters, and hybridized to 32P-labeled rRNA or cDNA. Fragments containing the 18S or 28S rRNA coding sequences occurred in three major size classes; all three were rich in 5-methylcytosine. Analysis of EcoRI fragments of DNA from the tumor and control cells after digestion with Hpa II or Msp I endonuclease indicated that the 5'-C-C-G-G-3' sequences in most of the amplified rRNA genes were methylated. Analysis of the fragments produced by digestion with Hha I endonuclease indicated a high degree of methylation within its recognition sequence in the amplified rRNA genes as well. The association of hypermethylation with restricted transcriptional activity suggests that DNA methylation may regulate the activity of the rRNA genes.Genes for mammalian 18S and 28S rRNA (rDNA) occur in multiple copies that are clustered at one or more sites per haploid genome. These sites can be visualized by in situ hybridization (1, 2) or a silver staining technique (3,4 The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.489

show abstract

The use of antinucleoside antibodies to probe the organization of chromosomes denatured by ultraviolet irradiation

Cited by 60 publications

References 24 publications

Chromatin structure and specificity revealed by immunological techniques

Chromatin structure and specificity revealed by immunological techniques

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

Amplified ribosomal RNA genes in a rat hepatoma cell line are enriched in 5-methylcytosine.

Contact Info

Product

Resources

About