A number of individuals with aberrant Y chromosomes have been tested for the presence of Y-chromosome-specific reiterated DNA. These studies locate Y-chromosome-specific reiterated sequences on the long arm of the Y chromosome. Correlation with phenotype and other known Y chromosome markers establish that the Y-chromosome-specific reiterated DNA discussed here has no evident role in male determination.
28S ribosomal RNA (rRNA) (2-4), suggesting that the absence of the human rRNA may be related to the loss of human chromosomes. It is not due to the absence of the human acrocentric chromosomes, which carry the structural genes for rRNA (5, 6), because Marshall et al. (4) found no human 28S rRNA in a large series of mousehuman hybrids that contained 2 to 11 human acrocentrics.The chromosome regions that carry the rRNA genes have been identified as the nucleolus organizer regions (NORs) (7), and these regions can be stained preferentially by the Ag-AS silver staining method (8). In human diploid cell cultures the Ag-AS method stains the short arm regions of most of the acrocentric chromosomes (9, 10). The NORs of the same human acrocentric chromosomes are not stained in a mouse-human hybrid that has lost some human chromosomes (11). There is no evidence to suggest that rRNA genes are deleted from the human acrocentrics in hybrid cells. Therefore the absence.of Ag-AS stain suggests that this method detects only chromosome regions that functioned as nucleolus organizers in the preceding interphase, and, by implication, produced rRNA.Somatic cell hybrids between either mouse peritoneal macrophages or mouse cells obtained from a teratocarcinoma and HT-1080 human fibrosarcoma cells retain human chromosomes and lose mouse chromosomes (12). If preferential chromosome elimination is closely correlated with preferential suppression of nucleolus organizer activity, these hybrids should express only human nucleolus organizer activity. This appears to be the case. METHODS BALB/c mouse peritoneal macrophages were obtained according to a modification of the procedure described by Cohn and Benson (13) and were fused with HT-1080-6TG human fibrosarcoma cells deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) (12) in the presence of f3-propiolactone-inactivated Sendai virus at pH 8.0 (14). The fused cultures were maintained in hypoxanthine-aminopterin-thymidine (HAT) selective medium (15). Large colonies of hybrid cells were visible 3-4 weeks after fusion. The colonies were picked and, subsequently, grown. OTT6050 mouse teratocarcinoma cells were obtained from a solid teratocarcinoma of a strain 129 mouse (16,17) by cutting in small fragments in trypsin/EDTA, resuspending in Eagle's minimal essential medium (MEM), and filtering through sterile gauze. The teratocarcinoma cells were fused with HT-1080-6TG cells in the presence of f.-propiolactone-inactivated Sendai virus. Hybrid colonies were selected in hypoxanthine-aminopterin-thymidine medium.Hybrid cells were maintained in Eagle's medium supplemented with 10% fetal calf serum. Mitotic cells were shaken from the culture flasks and transferred to a centrifuge tube containing 0.1 ml of colcemid (10 Ag/ml) for every 10 ml of medium and the tubes were centrifuged immediately at 800 rpm in an IEC clinical centrifuge for 7 min. The cell pellet was resuspended in 75 mM KCI. After 10 min the suspension was centrifuged and the cells were fixed for an hour in freshly prepa...
Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.
The marked binding of antibodies specific for 5-methylcytidine to the short arm of chromosome 15 distinguishes this chromosome from the other human acrocentrics. This method has been used to study over 60 individuals including 12 who did not have Down's syndrome, but who did have an extra G-group sized acrocentric chromosome. In six cases the extra chromosome did not show intensive binding of anti-5-methylcytidine. In the other six cases, the extra chromosome contained a 5-methylcytidine rich band at each end indicating that both ends were derived from chromosome 15 and contained centromeric heterochromatin normally present on the short arm of chromosome 15. The duplication of short arm material in the abnormal chromosomes was confirmed in all cases by quinacrine staining, nucleolar organizer (Ag-AS) staining or C-banding. In three cases, the abnormal chromosome appeared to arise from two different chromosomes 15. Several possible mechanisms for the production of the abnormal chromosome are discussed. The individuals with this abnormal chromosome all showed some degree of mental retardation, but few common physical findings.
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