The organization of the mouse satellite DNA at centromeres

Joseph, Ann Mary; Mitchell, A. R.; Miller, Orlando J.

doi:10.1016/0014-4827(89)90408-4

Cited by 104 publications

(58 citation statements)

References 13 publications

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“…Major murine satellite repeats (corresponding to ␥-satellite sequences) share with the human ␣-satellite sequences the same centromere localization, the same (A+T)-rich base content and the same heterochromatin structure (Joseph et al, 1989;Choo, 1997;Craig et al, 2003;Guenatri et al, 2004). In order to investigate the capacity of tau to interact with murine ␥-satellite DNA, we performed EMSA experiments under the same conditions as those used in Fig.…”

Section: Resultsmentioning

confidence: 99%

Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

Sjöberg

Shestakova

Mansuroglu

et al. 2006

Journal of Cell Science

139

143

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The microtubule-associated tau protein participates in the organization and integrity of the neuronal cytoskeleton. A nuclear form of tau has been described in neuronal and non-neuronal cells, which displays a nucleolar localization during interphase but is associated with nucleolar-organizing regions in mitotic cells. In the present study, based on immunofluorescence, immuno-FISH and confocal microscopy, we show that nuclear tau is mainly present at the internal periphery of nucleoli, partially colocalizing with the nucleolar protein nucleolin and human AT-rich α-satellite DNA sequences organized as constitutive heterochromatin. By using gel retardation, we demonstrate that tau not only colocalizes with, but also specifically binds to, AT-rich satellite DNA sequences apparently through the recognition of AT-rich DNA stretches. Here we propose a functional role for nuclear tau in relation to the nucleolar organization and/or heterochromatinization of a portion of RNA genes. Since nuclear tau has also been found in neurons from patients with Alzheimer's disease (AD), aberrant nuclear tau could affect the nucleolar organization during the course of AD. We discuss nucleolar tau associated with AT-rich α-satellite DNA sequences as a potential molecular link between trisomy 21 and AD.

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Section: Resultsmentioning

confidence: 99%

Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

Sjöberg

Shestakova

Mansuroglu

et al. 2006

Journal of Cell Science

139

143

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“…Despite the fact that the absolute length of these long, uninterrupted tandem arrays is not known (because of an inherent mapping/assembly problem after sequencing), the number of targeted QDs appears far less than the estimated several thousands of tandem repeats of satellite DNA monomers organized in discrete blocks at the centromere of mouse chromosomes (6,10). Three main factors might account for the apparent paucity of QDs seen at the FISH signals.…”

Section: Discussionmentioning

confidence: 99%

Single-Step Multicolor Fluorescence In Situ Hybridization Using Semiconductor Quantum Dot-DNA Conjugates

Bentolila

Weiss

2006

CBB

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We report a rapid method for the direct multicolor imaging of multiple subnuclear genetic sequences using novel quantum dot-based fluorescence in situ hybridization (FISH) probes (QD-FISH). Short DNA oligonucleotides were attached on QDs and used in a single hybridization/ detection step of target sites in situ. QD-FISH probes penetrate both intact interphase nuclei and metaphase chromosomes and showed good targeting of dense chromatin domains with minimal steric hindrances. We further demonstrated that QD's broad absorption spectra allowed different colored probes specific for distinct subnuclear genetic sequences to be simultaneously excited with a single excitation wavelength and imaged free of chromatic aberrations in a single exposure.Thus, these results demonstrate that QD-FISH probes are very effective in multicolor FISH applications. This work also documents new possibilities of using QD-FISH probes detection down to the single molecule level.

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“…The mouse genome contains two classes of centromeric repetitive sequences, termed major and minor satellites, organized in largely uninterrupted blocks of tandem repeats. Cytological detection of these clusters shows distinct localization, major satellites being associated with pericentromeric regions, whereas minor satellites were detected at the primary constriction of condensed mitotic chromosomes (6,7). The nature of protein complexes recruited to these satellite repeats also appears to be distinct.…”

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confidence: 97%

Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function

Bouzinba-Ségard

Ferri

Francastel

2007

Blood Cells, Molecules, and Diseases

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RNAs have been implicated in the assembly and stabilization of large-scale chromatin structures including centromeric architecture; unidentified RNAs are integral components of human pericentric heterochromatin and are required for localization of the heterochromatin protein HP1 to centromeric regions. Because satellite repeats in centromeric regions are known to be transcribed, we assessed a role for noncoding centromeric RNAs in the structure and function of the centromere. We identified minor satellite transcripts of 120 nt in murine cells that localize to centromeres and accumulate upon stress or differentiation. Forced accumulation of 120-nt transcripts leads to defects in chromosome segregation and sister-chromatid cohesion, changes in hallmark centromeric epigenetic markers, and mislocalization of centromere-associated proteins essential for centromere function. These findings suggest that small centromeric RNAs may represent one of many pathways that regulate heterochromatin assembly in mammals, possibly through tethering of kinetochore-and heterochromatin-associated proteins.centromere ͉ centromeric transcripts ͉ kinetochore

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The organization of the mouse satellite DNA at centromeres

Cited by 104 publications

References 13 publications

Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

Single-Step Multicolor Fluorescence In Situ Hybridization Using Semiconductor Quantum Dot-DNA Conjugates

Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function

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