The use of an engineered single chain variable fragment in a localized surface plasmon resonance method for analysis of the C-reactive protein

Byun, Ju-Young; Shin, Yong‐Beom; Li, Taihua; Park, Jinho; Kim, Dong‐Myung; Choi, Dong-Hwan; Kim, Min-Gon

doi:10.1039/c3cc45046e

Cited by 28 publications

(21 citation statements)

References 27 publications

(2 reference statements)

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“…The GNBP substrate was fabricated by using the following, previously described procedure [8]. Glass slides (25 mm x 10 mm x 0.15 mm) were cleaned with piranha solution at 65 C for 30 min, rinsed with distilled water (DW) and ethanol, and immersed in an ethanolic 2% APTMS (Sigma-Aldrich, 281778) solution at room temperature for 1 h. The amine modified slide was treated with 1 M succinic anhydride (Sigma-Aldrich, 239690) in DMF (Sigma-Aldrich, 227056) at 37 C for 12 h and then washed with DW.…”

Section: Fabrication Of Gnbp Substratementioning

confidence: 99%

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A localized surface plasmon resonance (LSPR) immunosensor for CRP detection using 4-chloro-1-naphtol (4-CN) precipitation

Park

Byun

et al. 2017

SPIE Proceedings

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In this study, C-reactive protein (CRP) was detected by monitoring of LSPR shift promoted by precipitation of 4-chloro-1-naphthol (4-CN). The precipitation occurred by horseradish peroxide (HRP) catalyst which is modified at CRP-detection antibody utilized in sandwich enzyme-linked immunosorbent assay (ELISA) on gold nano bipyramid (GNBP) substrate. Due to 4-CN precipitates which are located nearby the surface of GNBP, local refractive index (RI) and molecular density were greatly increased. This phenomenon eventually induced strong spectral red-shift of absorption band of GNBP. An excellent linear relationship (R 2 =0.9895) between the LSPR shift and CRP concentration was obtained in the range from 100 pg/mL to 100 ng/mL and limit of detection (LOD) was reached to 87 pg/mL.

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Section: Fabrication Of Gnbp Substratementioning

confidence: 99%

“…This oscillation is sensitive to surrounding refractive index (RI) value and molecular density nearby NP. When biomolecule exists on the surface of NP, it leads to local RI change and this promotes extinction peak shift of NP [8]. By observing LSPR shift, biomolecular detection is possible.…”

Section: Introductionmentioning

confidence: 99%

A localized surface plasmon resonance (LSPR) immunosensor for CRP detection using 4-chloro-1-naphtol (4-CN) precipitation

Park

Byun

et al. 2017

SPIE Proceedings

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“…The GNR substrate was fabricated by using the following, previously described procedure [1]. Glass slides (50 mm x 35 mm x 0.15 mm) were cleaned with piranha solution at 65 °C for 30 min, rinsed with distilled water (DW) and ethanol, and immersed in an ethanolic 2% APTMS solution at room temperature for 1 h. The amine modified slide was treated with 1 M succinic anhydride in DMF at 37 °C for 12 h and then washed with DW.…”

Section: Fabrication Of Gnr Substratesmentioning

confidence: 99%

“…Biomolecular interactions at the surface of the nanostructures directly lead to local refractive index changes. The increase in refractive index of the nanoparticles leads to a red shift of the LSPR band, which is greatly affected by the attachment of biomolecules on the nanoparticle surface [1]. Therefore, monitoring spectral shift of scattering or absorption signal enables sensing the presence of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Dark-field spectral imaging microscope for localized surface plasmon resonance-based biosensing

2015

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Localized surface plasmon resonance (LSPR) of metal nanoparticles makes red-shift of extinction wavelength with an increase in the refractive index at the surface of the metal nanoparticles. Since biomolecules bound to the metal nanoparticle's surface induce refractive index change, biosensing based on LSPR effect can be possible by monitoring scattering or absorption spectrum changes. Generally, however, conventional method detects ensemble averaged LSPR signal of a huge number of metal nanoparticles. Here, we have constructed a dark-field spectral imaging microscope in order to monitor the scattering spectra of individual metal nanoparticles, simultaneously. Gold nanorod (GNR) and aptamer are employed to detect ochratoxin A (OTA) related to a carcinogenic illness. An aptamer-target binding mechanism promotes wavelength shift of extinction spectra due to refractive index change within sensing volume of GNR by structural change of aptamer. A number of GNRs can be identified in a dark-field LSPR image, simultaneously. A typical spectrum of a GNR exhibits red-shift after target binding of molecules and OTA detection is extended to the very low concentration of 1 pM level.

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“…On the contrary, however, the short penetration depth of LSP field can be a crucial demerit when large-sized antibody is utilized as a bioreceptor molecule specifically recognizing a target molecule such as an antigen [18], [19] The analyte molecules bound to large receptor molecules immobilized on the surface of plasmonic metal nanostructure are hardly detectable, even if they are still within the LSP field, which can make direct detection of target difficult especially at low concentration of target. Therefore, size reducing of receptor molecules is effective for improvement of the sensitivity in LSPR biosensing, which experimentally substantiated through a few research reports [20], [21] [22] As another approach to overcome this obstacle against LSPR biosensing, enzyme/precipitation reaction was employed on gold nanodots after the antigen-antibody reaction in the previous study [23]. The enzyme reaction derived from ELISA produced insoluble precipitates that have high dielectric constant through the bio-catalytic reaction.…”

Section: Introductionmentioning

confidence: 96%

Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA

Shin

Lee

2015

SPIE Proceedings

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We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively.We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50L human serum with limit of detection (LOD) of 0.7 zeptomole (10 -21 mole).

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The use of an engineered single chain variable fragment in a localized surface plasmon resonance method for analysis of the C-reactive protein

Cited by 28 publications

References 27 publications

A localized surface plasmon resonance (LSPR) immunosensor for CRP detection using 4-chloro-1-naphtol (4-CN) precipitation

A localized surface plasmon resonance (LSPR) immunosensor for CRP detection using 4-chloro-1-naphtol (4-CN) precipitation

Dark-field spectral imaging microscope for localized surface plasmon resonance-based biosensing

Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA

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