The use of a tannin crude extract from Cistus ladanifer L. to protect soya-bean protein from degradation in the rumen

Abstract

Cistus ladanifer L. (CL) is a perennial shrub abundant in dry woods and dry land of Mediterranean zone, with high level of tannins. Tannins bind to protein, preventing its degradation in the digestive compartments. This tannin/protein complex may be advantageous when partially protecting good-quality feed protein from excessive rumen protein degradation. The objective of this trial was to use a CL phenol crude extract to prevent excessive rumen degradation of soya-bean meal protein. The phenolic compounds were extracted using an acetone/water solution (70:30, v/v). Soya-bean meal was then treated with this crude CL extract, containing 640 g of total phenols (TP) per kg of dry matter (DM), in order to obtain mixtures with 0, 12.5, 25, 50, 100 and 150 g of TP per kg DM. Three rumen-cannulated rams were used to assess in sacco rumen degradability of DM and nitrogen (N). The three-step in vitro procedure was used to determine intestinal digestibility.

Effects of several levels of extracted tannin from grape pomace on intestinal digestibility of soybean meal

“…Although they found a decline in rumen degradability of protein, in vitro digestibility of the undegraded protein was not affected. Dentinho et al (2007) also found a reduction in the effective rumen degradability of SBM protein treated with Livestock Science 128 (2010) 87-91…”

Potential of Cistus ladanifer L. (rockrose) in small ruminant diets – effect of season and plant age on chemical composition, in vitro digestibility and antioxidant activity

“…Conversely, high CT concentrations may adversely affect dietary preference and are associated with reduced feed intake, digestibility of fibre and nitrogen, and animal performance (Min et al ., ; Makkar et al ., ; Waghorn, ). Incorporation of tannin crude extract from C. ladanifer in soya bean meal, to obtain diets with CT levels that vary between 0·6% to 7% in DM, is reported to reduce the rumen degradation of protein, as well as the protein intestinal digestibility (Dentinho et al ., , ). However, those studies did not evaluate whether this reduction in protein availability has implications for animal performance.…”

“…Cistus ladanifer L. (rockrose) is a perennial shrub which is widespread in marginal grazing lands in Mediterranean countries (Dentinho et al ., ). Cistus ladanifer is considered an unbalanced feed with poor nutritional value, showing high levels of antinutritional factors, as condensed tannins (CT) (Dentinho et al ., ).…”

The effect of oak tannin (Quercus robur) and hops (Humulus lupulus) on dietary nitrogen efficiency, methane emission, and milk fatty acid composition of dairy cows fed a low-protein diet including linseed

“…Several in situ studies have demonstrated the reduction of protein degradation in the rumen. In particular, the degradation rate of soybean N decreased linearly with the level of Cistus ladanifer extract (Dentinho et al, 2007) or with treatment with quebracho tannins (Frutos et al, 2000).…”