The use of a collagen sponge/living cell composite material to treat donor sites in burn patients

Still, Joseph M.; Glat, Paul M.; Silverstein, Paul; Griswold, John; Mozingo, D.W.

doi:10.1016/s0305-4179(03)00164-5

Cited by 219 publications

(116 citation statements)

References 8 publications

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“…The limited availability of donor skin for autograft in large burns, and the risks of rejection and transmissible diseases associated with allograft, have led to the development of tissue engineered skin equivalents as alternatives in burn wound treatment. Various approaches have been taken towards the engineering of these therapeutic materials, including cultured epithelial autografts(1;2) (Epicel ™ ); acellular dermal substitutes(3-6) (IntegraDRT ™, AlloDerm ™ ), cell populated dressings(7) (TransCyte ™ ); cellular dermal substitutes(8) (Dermagraft ™ ); and bilayered skin substitutes (9), (10) (Apligraf ™ , Orcel ™ ).…”

Section: Introductionmentioning

confidence: 99%

Wound Healing on Athymic Mice With Engineered Skin Substitutes Fabricated with Keratinocytes Harvested from an Automated Bioreactor

Kalyanaraman

Boyce

2009

Journal of Surgical Research

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The Kerator is a computer controlled bioreactor for the automated culture and harvest of keratinocytes that can reduce labor and materials involved in the fabrication of engineered skin substitutes (ESS). Previous studies have shown that Kerator is comparable to tissue culture flasks by keratinocyte confluence during culture, clonogenic potential of harvested keratinocytes and microanatomy, cell viability and surface hydration of ESS fabricated with the harvested keratinocytes. In this study, the Kerator and tissue culture flasks were further compared by keratinocyte proliferation in vitro and wound healing after transplantation of ESS to athymic mice. The number of BrdU positive keratinocytes in ESS fabricated with keratinocytes harvested from Kerator after 2 weeks of in vitro maturation was 34 ± 3 per high power field (hpf) (mean ± SEM), which was not significantly different from that fabricated with keratinocytes harvested from flasks (34 ± 1.5 per hpf). Percentage original wound area 6 weeks after surgery of ESS fabricated with keratinocytes from the Kerator was 36 ± 3.3%, which was not significantly different from that of ESS fabricated with keratinocytes from flasks (30 ± 4.3%). In both cases, 78% (7 of 9) mice transplanted were positive for engraftment of human keratinocytes by direct immunofluoresence for HLA-ABC antigens. These results further confirm that the ESS fabricated with keratinocytes harvested from Kerator and flasks are equivalent in vitro and in vivo. Therefore, use of Kerator for large scale production of ESS can lead to increased availability at reduced cost while maintaining ESS quality for grafting.

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Section: Introductionmentioning

confidence: 99%

Wound Healing on Athymic Mice With Engineered Skin Substitutes Fabricated with Keratinocytes Harvested from an Automated Bioreactor

Kalyanaraman

Boyce

2009

Journal of Surgical Research

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“…Type I pig collagen forms an inner layer with an inert, hydrophilic network structure and provides a www.intechopen.com suitable platform for the development of granulation tissue. Water transfer can be maintained similar to that of natural skin by modifying the membrane thickness (Suzuki et al, 1990;Ou et al, 1998;Still et al, 2003). Fig.…”

Section: Compositesmentioning

confidence: 87%

“…Much research is currently being undertaken studies to develop wound dressing materials that can provide optimum healing conditions, taking into account all of these factors and healing mechanisms (inflammation, tissue replacement, fibrosis, coagulation, etc.) (Still et al, 2003;Stashak et al, 2004). Some of artificial wound and burn dressing materials in world market are shown in Table 4.…”

Section: Artificial Dressingsmentioning

confidence: 99%

Biopolymers as Wound Healing Materials: Challenges and New Strategies

Sezer¹,

Cevher²

2011

Biomaterials Applications for Nanomedicine

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“…Their important properties are a specified mechanical strength, which provides the possibility to use them as a base for tissue-engineered constructs, and ability to biodegradation in the body. Such equivalents on the sponge matrix are effectively employed for skin regeneration in the experiments on animals and in treating skin wounds in patients [58][59][60].…”

Section: Biopolymer Matricesmentioning

confidence: 99%

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

Meleshina¹,

Bystrova²,

Rogovaya³

et al. 2017

Sovrem Tehnol Med

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Development and introduction of new biotechnological analogues (equivalents) of tissues and organs into clinical practice, such as human skin equivalents (SE), designed for temporal or permanent replacement of damaged or destroyed tissue, remains an urgent problem of regenerative medicine. Currently, full-thickness SE as well as separate skin layers, which include living cells of different types, are being created and investigated. Since the ideal skin substitutes have not been created, the efforts of researchers in many countries are aimed at solving this problem.In our review, we present a comparative analysis of existing SE, both commercial and those being at the stage of preclinical study, analyze their structure and feasibility of application for solving experimental and clinical tasks. Characteristics of the three main variants of SE have also been considered. Examples of stem cell application for creation of skin equivalents have been given. The main advantages of using stem cells as a cell component of skin equivalents have been described.

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The use of a collagen sponge/living cell composite material to treat donor sites in burn patients

Cited by 219 publications

References 8 publications

Wound Healing on Athymic Mice With Engineered Skin Substitutes Fabricated with Keratinocytes Harvested from an Automated Bioreactor

Wound Healing on Athymic Mice With Engineered Skin Substitutes Fabricated with Keratinocytes Harvested from an Automated Bioreactor

Biopolymers as Wound Healing Materials: Challenges and New Strategies

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

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