The uptake of cortisol by rat tissues

The Effect of Cortisol on Body Weight and Muscle Metabolism in the Rat

“…Groups of animals were killed at intervals after the injection and the externus vastus lateralis muscles removed and stored at -10°. Cortisol and its metabolites were extracted from muscle according to Bellamy, Phillips, Chester Jones & Leonard (1962), and measured with either a TMC model LP 2 A liquid phosphor coincidence counter (Technical Measurement Corporation, Conn., U.S.A.) or a Nuclear Chicago liquid scintillation counter (Nuclear Chicago Corporation, Illinois, U.S.A.). The Chromatographie method of Bellamy (1963) with the following modifications was used to measure unchanged cortisol.…”

Subcellular Distribution of the (3H) Corticosterone Fraction in Brain, Thymus, Heart, and Liver of the Rat.

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Several investigators have studied the subcellular distribution of labeled glucocorticoids in vivo and in vitro in rat tissues (2,3,4,6,7,9).

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Mechanism of Hormonal Action

“…The presence of a steroid concentrating mechanism in liver has been shown by Szego [85], Sandberg, Slaunwuite and Antoniades [60], It has been shown that liver has the highest distribution ratio for cortisol and cortico sterone of any organ tested in the rat [3], The uptake of tritiated corticoster one into liver slices has been shown to be a passive process reaching a maximum within 6 hours and corresponds to a six fold increase in concen tration, a large amount of the radioactivity being associated with more polar metabolites. At a temperature of 4°, Bellamy el al.…”