The ultrastructural effects of surgical treatment of hydrosalpinx on the human endometrium: a light and electron microscopic study

Sapmaz, Tuğçe; Gundogdu, Leman Sencar; Çetin, Mehmet Turan; Ürünsak, İbrahim Ferhat; Polat, Sait

doi:10.1080/01913123.2019.1600087

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…Additionally, changes in tubal epithelium composition can also lead to a form of female infertility known as tubal factor infertility, which in some women can be associated with hydrosalpinx -an enlarged and fluid-filled fallopian tube (Sapmaz et al 2019;Ajonuma et al 2005;Yohannes et al 2019). For these women, tubal factor infertility treatment involves the surgical removal of hydrosalpinx, followed by in vitro fertilization and embryo transfer (Strandell 2000;Johnson et al 2010;Sapmaz et al 2019). We currently have a limited understanding of the etiology of hydrosalpinx, and we lack alternative therapies (other than surgical removal) that could retain tubal function and allow unassisted fertility.…”

mentioning

confidence: 99%

Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified by scRNA-seq

Ulrich

Shen

et al. 2021

Preprint

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Fallopian tube (FT) homeostasis requires dynamic regulation of heterogeneous cell populations and is disrupted in infertility and ovarian cancer. Here we applied single-cell RNAseq to profile 53,376 FT cells from 3 healthy pre-menopausal subjects. The resulting cell atlas contains 12 major cell types representing epithelial, stromal and immune compartments. Re-clustering of epithelial cells identified 4 ciliated and 6 non-ciliated secretory epithelial subtypes, two of which represent potential progenitor pools: one leading to mature secretory cells, while the other contributing to either ciliated cells or one of the stromal cell types. To understand how FT cell numbers and states change in a disease state, we analyzed ~15,000 cells from a hydrosalpinx sample and observed shifts in epithelial and stromal populations, and cell type-specific changes in extracellular matrix and TGF-β signaling, underscoring fibrosis pathophysiology. This resource is expected to facilitate future studies to understand fallopian tube homeostasis in normal development and disease.

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mentioning

confidence: 99%

Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified by scRNA-seq

Ulrich

Shen

et al. 2021

Preprint

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“…When the fluid gets accumulated in the fallopian lumen, it tends to negatively impact the minor changes in nucleus, proliferation of the intraepithelial macrophages and lymphocytes, increased heterochromatin, multiple functions of endometrial cells and the vacuolation of the apical endoplasmic reticulum cisterns [ 13 ]. This may be attributed to the occurrence of low or reduced cell division.…”

Section: Discussionmentioning

confidence: 99%

Study of hydrosalpinx on endometrial growth and expression of HOXA10mRNA and related factors

Cai

Liu

et al. 2023

Heliyon

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“…Результати нашого дослідження показали, що при тривалому впливі опіоїду розвиваються патологічні процеси в матковій трубі, її слизова оболонка зазнає змін. Шеститижневе введення «налбуфіну» зумовлює перебудову структурної організації стінки маткової труби подібно до тривалих запальних процесів [18,19], зокрема збільшення просвіту маткових труб, зменшення або відсутність складок на певних ділянках маткової труби, зменшення кількості мікроворсинок трубних екзокриноцитів, виникнення аденоматозної проліферації трубного епітелію. Війчасті епітеліоцити десквамовані.…”

Section: «Art Of Medicine»unclassified

Scanning Electronic Microscopy of Mucous Membrane of the Uterine Tube Under the Conditions of Physiological Norm and Six-Week Administration of Opioid

Podolyuk

Матешук-Вацеба

Mykhalevych

et al. 2022

Scientific and practical journal

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The toxic effects of opioids on the female reproductive system are important for theoretical and practical medicine, because excessive use of narcotic drugs causes pathological changes in organs and tissues and can predict the negative effects of "nalbuphine" on the female genitals. The excessive use of narcotic drugs, the prevalence of drug addiction, the use of opioids by modern medicine for therapeutic purposes, creates the need to carefully study the effects of opioids on the body of women, primarily the organs of the reproductive system, because despite the successes achieved in diagnosis and treatment, female infertility is an urgent problem. Aim of the research: to establish and describe the features of the structural organization of the mucous membrane of the uterine tube of a female laboratory rat in normal and under conditions of prolonged exposure to «nalbuphine» according to the results of scanning electron microscopy. Materials and methods. The material of the research was the mucous membrane of the uterine tubes of 20 mature white female rats, aged 4.5–6.0 months with body weight 180–220, which were kept in standard conditions at the vivarium of the Danylo Halytskyi Lviv National Medical University. The structural organization of the mucous membrane of the uterine tubes was normally studied in 5 animals. Experimental animals were divided into 2 groups. The first group of experimental animals (10 rats) was administered «nalbuphine» intramuscularly once daily, in one period of time for 6 weeks. The second group - control animals - 5 white female rats, which were injected with saline. The method of scanning electron microscopy of the mucous membrane of the uterine tube of the rat was used in the research. Results. Lesions of the reproductive system damage is an integral part of clinic both opiate addiction and intoxication of pharmaceutical remedies in particular the group of opioids. The connection between duration of «nalbuphine» administration and depth of changes of structural organization of mucous membrane of the uterine tube was established in the experiment. The obtained data show that the six-week administration of «nalbuphine» causes destructive changes in the mucous membrane of the uterine tube wall similar to long-term inflammatory processes, in particular, an increase in the lumen of the uterine tube, a decrease or absence of folds in certain areas of the uterine tube, a decrease in the number of microvilli of tubal exocrinocytes, and the occurrence of adenomatous proliferation of the tubal epithelium. The damage that has occurred indicates the ability of the opioid to cause irreversible changes with prolonged use. Conclusions. The connection between duration of «nalbuphine» administration and depth of changes of structural organization of the mucous membrane of the uterine tube was established in the experiment. Prolonged administration of opioids causes irreversible destructive changes in the mucous membrane of the uterine tube of the female rat, which is manifested by the phenomena of disorganization of both endothelial cells. The damage that has occurred proves the ability of opioid in long-term use to cause irreversible changes and structure and function damages on all levels starting with structural. The results of the work can serve as a basis for the further search for optimal methods of correcting changes in the structure of organs caused by the long-term use of opioids.

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The ultrastructural effects of surgical treatment of hydrosalpinx on the human endometrium: a light and electron microscopic study

Cited by 7 publications

References 19 publications

Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified by scRNA-seq

Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified by scRNA-seq

Study of hydrosalpinx on endometrial growth and expression of HOXA10mRNA and related factors

Scanning Electronic Microscopy of Mucous Membrane of the Uterine Tube Under the Conditions of Physiological Norm and Six-Week Administration of Opioid

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