“…Each linear-type sgRNA is a 14-nt sequence complementary to a sequence 5 0 to the desired cleavage site, and can form a 14-bp doublestranded RNA with the target RNA, which roughly corresponds to a combination of the acceptor and T stems. 13 An siRNA, siRLuc, targeting downstream to one of the sites, which has been shown to work very efficiently, 17 was used as a positive control, and an unrelated 14-nt linear RNA, sgGSKL2 (5 0 -CUUAGUCCA AGGAU-3 0 ), was used as a negative control.…”