Human cytosolic tRNase Z^L can downregulate gene expression through miRNA

Abstract

Edited by Tamas Dalmay Keywords:A long form of tRNase Z TRUE gene silencing Small guide RNA MicroRNA Non-coding RNA RNA-induced silencing complex a b s t r a c t A long form of tRNase Z (tRNase Z L ) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including $25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase Z L in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3 0 untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase Z L . The present data suggest the possibility that human cytosolic tRNase Z L modulates gene expression through a subset of microRNAs in the cells.

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