Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Elbarbary, Reyad A.; Takaku, Hiroaki; Tamura, Masato; Nashimoto, Masayuki

doi:10.1016/j.bbrc.2008.12.173

Cited by 22 publications

(18 citation statements)

References 22 publications

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“…Since GSK-3β is known to mediate canonical Wnt signaling, we examined the effect of knockdown of GSK-3β using either RNA interference or tRNase Z L -utilizing efficacious gene silencing (TRUE gene silencing) (Tamura et al, 2003, Nakashima et al, 2007, Elbarbary et al, 2009) upon CXCL12 induction.…”

Section: Knock-down Of Gsk-3β Decreases Cxcl12 Productionmentioning

confidence: 99%

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Tamura

Sato

Nashimoto

2011

The International Journal of Biochemistry & Cell Biology

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Section: Knock-down Of Gsk-3β Decreases Cxcl12 Productionmentioning

confidence: 99%

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Tamura

Sato

Nashimoto

2011

The International Journal of Biochemistry & Cell Biology

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“…Again, tRNase Z S can process pre-tRNAs but not the pre-tRNA-like or micro-pre-tRNA-like complexes in vitro [6]. We have also shown the efficacy of this RNA targeting method in the living mammalian cells by introducing sgRNAs either as their expression plasmids or as 2′- O -methyl RNAs [13]–[16]. We call this method TRUE gene silencing after tR Nase Z L - u tilizing e fficacious gene silencing.…”

Section: Introductionmentioning

confidence: 96%

Modulation of Gene Expression by Human Cytosolic tRNase ZL through 5′-Half-tRNA

et al. 2009

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A long form (tRNase ZL) of tRNA 3′ processing endoribonuclease (tRNase Z, or 3′ tRNase) can cleave any target RNA at any desired site under the direction of artificial small guide RNA (sgRNA) that mimics a 5′-half portion of tRNA. Based on this enzymatic property, a gene silencing technology has been developed, in which a specific mRNA level can be downregulated by introducing into cells a synthetic 5′-half-tRNA that is designed to form a pre-tRNA-like complex with a part of the mRNA. Recently 5′-half-tRNA fragments have been reported to exist stably in various types of cells, although little is know about their physiological roles. We were curious to know if endogenous 5′-half-tRNA works as sgRNA for tRNase ZL in the cells. Here we show that human cytosolic tRNase ZL modulates gene expression through 5′-half-tRNA. We found that 5′-half-tRNAGlu, which co-immunoprecipitates with tRNase ZL, exists predominantly in the cytoplasm, functions as sgRNA in vitro, and downregulates the level of a luciferase mRNA containing its target sequence in human kidney 293 cells. We also demonstrated that the PPM1F mRNA is one of the genuine targets of tRNase ZL guided by 5′-half-tRNAGlu. Furthermore, the DNA microarray data suggested that tRNase ZL is likely to be involved in the p53 signaling pathway and apoptosis.

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“…The present results together with this observation imply that other types of small cellular ncRNA would also mediate regulation of gene expression by tRNase Z L . It is now clear that the reason why the TRUE gene silencing method works well in the cells [14][15][16][17] is because it utilizes artificial sgRNAs like naturally occurring ncRNAs and mimics the intrinsic cellular gene regulatory system. …”

Section: A New Mechanism For Mirna-mediated Silencingmentioning

confidence: 99%

Human cytosolic tRNase Z^L can downregulate gene expression through miRNA

et al. 2009

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Edited by Tamas Dalmay Keywords:A long form of tRNase Z TRUE gene silencing Small guide RNA MicroRNA Non-coding RNA RNA-induced silencing complex a b s t r a c t A long form of tRNase Z (tRNase Z L ) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including $25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase Z L in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3 0 untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase Z L . The present data suggest the possibility that human cytosolic tRNase Z L modulates gene expression through a subset of microRNAs in the cells.

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Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Cited by 22 publications

References 22 publications

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Modulation of Gene Expression by Human Cytosolic tRNase ZL through 5′-Half-tRNA

Human cytosolic tRNase Z^L can downregulate gene expression through miRNA

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Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Cited by 22 publications

References 22 publications

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Modulation of Gene Expression by Human Cytosolic tRNase ZL through 5′-Half-tRNA

Human cytosolic tRNase ZL can downregulate gene expression through miRNA

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Human cytosolic tRNase Z^L can downregulate gene expression through miRNA