The Study of Blood Creatinin and Urea Concentration of Wistar Rats (Rattus norvegicus) Due to Sodium Nitrite Induction

Widyastuti, Dyah Ayu; Ristianti, Maria Asih Ristianti Maria Asih; Sari, Ika Maya Sari Ika Maya

doi:10.35814/jifi.v17i1.560

Cited by 5 publications

(4 citation statements)

References 8 publications

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“…High serum creatinine levels indicate failure of creatinine filtration in the kidney. However, both the decrease and increase of creatinine levels are not always caused by poor kidney function but also due to certain factors such as muscle mass change or drugs [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

et al. 2021

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Background and Aim: Hydrogen peroxide (H2O2) is a free radical, widely use as a food preservative, may cause adverse effects to the body. Mangosteen contains various antioxidants may scavenge free radical produced by H2O2. This study examined the effects of mangosteen peel infusion on the liver and kidney of rats after H2O2 induction. Materials and Methods: Thirty-six female Wistar rats were divided into six groups: Negative control, positive control (1% H2O2), as well as mangosteen peel infusion groups at a concentration of 0.25; 0.5; 1, and 2%. H2O2 induction was administered orally every day for 2 months followed by mangosteen peel infusion treatment (1 month) after H2O2 induction for 1 month. At the end of the experiment, the rats were sacrificed. The liver and kidney of each rat were collected for reactive oxygen species (ROS) and histopathological examinations. Furthermore, blood was collected for creatinine examination. Results: H2O2 induction caused the highest ROS level elevation in the positive control group which was treated with H2O2 only. Meanwhile, 2% of mangosteen peel infusion returned the ROS levels to normal. It was also observed that mangosteen peel infusion restored creatinine levels to normal. Furthermore, 2% of mangosteen peel infusion improved the histopathological structure of the liver and kidneys after H2O2 induction. Conclusion: Mangosteen peel infusion especially at a concentration of 2% has the potential to improve liver and kidney structure and functions after H2O2 induction.

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Section: Resultsmentioning

confidence: 99%

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

et al. 2021

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“…Overweight or obesity in rats can also affect kidney function, thereby affecting levels of BUN and serum creatinine. (24) Therefore, the use of rats in this study should be carried out in a weight range that is not too wide and varied. The difficulty in this study is that there is a rapid increase in rat body weight during the adaptation period, so that the range of rat body weight is quite wide, namely 150-300 grams.…”

Section: Discussionmentioning

confidence: 99%

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

2020

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Background Diazinon is a widely used pesticide to eradicate agricultural pests globally with the potential to cause harmful effects on humans by generating free radicals and depleting endogenous antioxidants. Soy flour possesses high antioxidant effect. This study evaluated the nephroprotective effect of soy flour on diazinon-induced nephrotoxicity in rats. Methods An experimental laboratory study was carried out involving 30 male Wistar rats were randomly divided into five groups of 6 rats. The normal control (Kn) and the negative control (K-) groups received normal saline, the treatment groups (K1, K2, K3) received 10%, 15%, and 20% soy flour in distilled water, respectively, on day 1 to day 28. Subsequently, for five days, all groups received diazinon 40 mg/kg, except for the normal control group. Blood urea nitrogen (BUN) and creatinine levels were measured at the end of the study. The Kruskal Wallis test with the post hoc Mann Whitney U test were used to analyze the data. Results Blood urea nitrogen and creatinine levels were lower in all treatment groups (K1, K2, K3) compared to the negative control group (K-), while the K3 level was the lowest (24.28 ± 1.56 mg/dL and 0.93 ± 0.14 mg/dL). There was a significant decrease in BUN and creatinine levels in the experimental rats of the 15% and 20% soy flour groups (K2, K3) compared to the negative control group (K-). Conclusion Administration of soy flour (Glycine max (L.) Merr.) had a nephroprotective effect in preventing increases in BUN and creatinine levels of diazinon-induced nephrotoxic male Wistar rats.

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“…Oleh karena itu, peningkatan kadar BUN di dalam darah menjadi salah satu indikator terjadinya gangguan pada ginjal (Mohan et al, 2015). Kadar BUN normal pada tikus Wistar adalah 13,37-21,49 mg/dL (Widyastuti et al, 2019). Pada Gambar 2 juga terlihat adanya peningkatan nilai rerata kadar BUN semua kelompok perlakuan jika dibandingkan dengan kadar BUN baseline, namun peningkatan kadar BUN pada kelompok EEBP dosis 50, 120, dan 300 mg/kgBB masih dalam rentang batas normal.…”

Section: Hasil Dan Pembahasanunclassified

Efek Nefroprotektif Ekstrak Etanol Bawang Putih (Allium sativum L.) pada Tikus yang Diinduksi Gentamisin

Sujono

Rizki

2020

Pharmacon

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Gentamicin can induce damage to the kidney because of the accumulation of epithelial cells in the cortex. This accumulation can cause an increase in Reactive Oxygen Species (ROS), which in turn can trigger an increase in free radicals which causes a decrease in kidney function, which can be seen with Blood Urea Nitrogen (BUN) and sCr (serum creatinine) parameters. Garlic (Allium sativum L.) contains several bioactive compounds, including alliin, allisin, ajoene, and allil cysteine. The content of allisin has activity as an antioxidant. This study used a nephrotoxic mouse modelling method with 100 mg/kgBW of gentamicin induction intraperitoneally, then BUN and sCr levels were checked 24 hours after the 10th day induction. This study aimed to determine the effect of garlic ethanol extract in preventing the decline of renal function in rats induced by gentamicin with a parameter of BUN and sCr. The animal test were divided into 5 treatment groups, normal control group, negative controls, and three treatment groups at a dose of 50, 120, and 300 mg/kgBW for 10 days of concurrent treatment between extracts with gentamicin. The results of the study showed that garlic ethanol extract with a dose of 50, 120, 300 mg/kgBW was able to prevent a decrease in kidney function due to the administration of gentamicin significantly when compared with negative controls (p 0,05).

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The Study of Blood Creatinin and Urea Concentration of Wistar Rats (Rattus norvegicus) Due to Sodium Nitrite Induction

Cited by 5 publications

References 8 publications

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

Efek Nefroprotektif Ekstrak Etanol Bawang Putih (Allium sativum L.) pada Tikus yang Diinduksi Gentamisin

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