The Study of a Disposable Reagentless Biosensor for Fast Test of Aspartate Aminotransferase

Abstract

A reagentless biosensor for amperometric measurement of aspartate aminotransferase (AST) has been developed. The three-electrode biosensor was modified with two enzyme membrane with an integrated planar Ag j AgCl reference electrode. With a cellulose membrane containing catalytic substance coated, the reagentless biosensor was used for measurement of serum AST. The optimal sensitive membrane composition and operation conditions were studied. With low polarizing potential (0 V vs. integrated Ag j AgCl) and appropriate substrate concentration (20 mM a-ketoglutarate acid), the response of the biosensor to AST activity was linear over the range of 25 -1000 U/L with good relativity (R ¼ 0.9904 and 0.9823 in standard AST solutions and AST serum respectively) and fast response time (120 s). The biosensor also indicated good stability of more than 90% of its original activity for 60 days stored in 4 8C, and 90% of the activity was retained after a storage period of 15 days at room temperature (25 8C). The biosensor is expected to be used for the diagnosis of heart and liver disease in the future.

Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases

“…This sensitivity was higher than the experimental results in the QC serum measurement reported by Guo et al . [ 10 ]. Serum quantification had the limit of detection LOD = 0.09 μg/mL, corresponding to an AST specific activity of 25.3 UL −1 .…”

“…Guo et al . [ 10 ] developed a hydrogen peroxide (H 2 O 2 ) sensor by integrating a glutamate electrode on which glutamate oxidase (GluOx) was co-immobilized with horseradish oxidase (HRP), pretreated with a redox polymer. Additionally, Ohgami et al .…”

Biomarkers and biosensors for the diagnosis of noncompliant pH, dark cutting beef predisposition, and welfare in cattle

“…This system can work in a reagent‐less electrochemical sensor since the substrate can be directly embedded in the sensor, reducing the sample handling steps. In this manner, AST can be detected in <5 min at biologically relevant levels, making this a promising approach (Chang & Chen, 2007; Guo et al., 2008; Hsueh et al., 2012).…”

Amperometric Biosensor for Diagnosis of Disease

“…Aspartate aminotranferase is an enzyme to diagnose acute myocardial infarction [109]. A biosensor based on Os-HRP layer and a layer composed by hydroxiethylcellulose, microcrystalline cellulose, aspartic acid, cetoglutaric acid and pyridoxil onto the gold electrode was proposed by Guo, et al [110]. The LOD was 10 U L -1 , shelf stability 2 months, response time 120 s. Adenosine deaminase (ADA) level is a biomarker for liver disease.…”