The strategy and current status of Brassica rapa genome project

문정환,; 권수진,; 박범석,

doi:10.5010/jpb.2010.37.2.153

Cited by 3 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Genome Project, MBGP)가 활발히 진행되어 방대한 유전체 정보가 해독되었으며, 이들 정보의 이용도 가능하게 되었다 (Mun et al, 2009(Mun et al, , 2010. 배추에서 이미 보고된 유전자 지 도에는 대량의 SSR 마커 정보가 집적되어 있으며 (Kim et al, 2006Li et al, 2010;Ramchiary et al, 2011), 최근 배추 유전체에 대한 초안이 발표되어 분자마커 개발에 도 활용이 가능하다 (Wang et al, 2011 unweightes pair group method using arithmetic average)을 이용하여 집괴분석(cluster analysis)을 하였다 (Rohlf, 1993;Sneath and Sokal, 1973).…”

Section: 으로 가까운 배추의 유전체 프로젝트(Multinational Brassicaunclassified

Analysis of the Genetic Diversity of Radish Germplasm through SSR Markers Derived from Chinese Cabbage

Park¹,

Choi²,

Lee³

et al. 2013

Korean Journal of Horticultural Science and Technology

View full text Add to dashboard Cite

Abstract. Since the early 1980s, the National Institute of Horticultural & Herbal Sciences has been breeding and collecting diverse radish breeds to select those samples with better horticultural characteristics, to ultimately expand and develop as good radish produce. Genetic diversity is a crucial factor in crop improvement and therefore it is very important to obtain various variations through sample collection. The collected samples were compared with one another in order to assess the level of diversity among the collections, and this procedure allowed for increased application of the gathered resources and aided in determining the direction to secure further samples. Towards this end, this experiment was conducted in order to examine whether the SSR markers derived from Chinese cabbage samples could be transferred to the radish samples. Among the radish breeding lines and introduced resources, 44 lines were used as materials to analyze the genotype using 22 SSR markers selected. As a result, the analysis showed that among all the selected markers, 'cnu_m139' and 'cnu_m289' were the most useful markers for diversity evaluation. The genetic relationship of the radish genetic resources showed that the geographic origins affected the diversity. Furthermore, the different types of radish groups were also determined by the year they were bred. This result demonstrated that there are differences between the older radish breeds and the more recently developed radish breeds. Even though a relatively small number of markers were used in the analysis, it was possible to distinguish whether the radish was bred 30 years ago or in the 2000s, and that the similar physical shapes comprised a particular group, showed that the SSR markers can indeed be successfully applied to to study the diversity within radish breeding lines. Through the results of this study, it can be concluded that the SSR marker developed for the Chinese cabbage can be applied to examine the genetic diversity and analyze the relationship (genetic resource determination) of radish.

show abstract

Section: 으로 가까운 배추의 유전체 프로젝트(Multinational Brassicaunclassified

Analysis of the Genetic Diversity of Radish Germplasm through SSR Markers Derived from Chinese Cabbage

Park¹,

Choi²,

Lee³

et al. 2013

Korean Journal of Horticultural Science and Technology

View full text Add to dashboard Cite

show abstract

“…무는 약 94%가 수분이며 단백질, 지질, 당질, 섬유질, 비타민, 철, 마그네슘, 칼슘, 매운맛 등 다양한 성분을 가 지고 있는 채소이다 (Mather 1977 (Mun et al 2009(Mun et al , 2010. 배추에서 이미 보고된 유전자 지도에는 대량의 SSR 마커 정보가 집적되어 있으며 (Kim et al 2006(Kim et al , 2009Li et al 2010;Ramchiary et al 2011) MSKD-A-1 0.10 0.12 0.11 0.09 0.10 0.08 0.07 MSKD-A-2 0.09 0.11 0.13 0.15 0.09 0.11 0.08 MSKD-A-3 0.05 0.04 0.05 0.07 0.04 0.05 0.04 MSKD-A-4 0.15 0.11 0.15 0.13 0.12 0.13 0.15 MSKD-A-5 0.12 0.13 0.11 0.12 0.12 0.10 0.14 MSKD-A-6 0.10 0.09 0.06 0.08 0.09 0.07 0.08 MSKD-A-7 0.07 0.08 0.07 0.06 0.10 0.08 0.09 Fig.…”

unclassified

Development of highly uniform variety for processing using SSR markers in radish (Raphanus sativus L)

정운화¹,

오종혁²,

김영규³

et al. 2014

Journal of Plant Biotechnology

View full text Add to dashboard Cite

Using commercial radish varieties for processing, about 30% of radish was discarded due to the root shape and low purity. To raise the processing ability, we tried to develop a new variety producing H-shaped root. As another characteristic required in variety for processing is high purity, we tried to raise purity using simple sequence repeats (SSR) markers for testing seed purity in every segregating generation. To develop Male-sterile (MS) seeding parent, we crossed commercial variety of 'Gwan dong spring' and 'Gyeo ryong spring'. One elite inbred was selected as recurrent parent for the MS plant. The major horticultural traits of selected inbred line were disease resistance, late bolting, heat resistance and bright green root top color. To develop pollen parent, we crossed commercial variety of 'Tae sang king' and 'Seoul spring'. We used individual selection method to develop H-shaped hard root and disease resistant inbred. In each segregating generation, we selected one plant based on phenotype and the uniformity of selected plant was tested by SSR markers using self-pollinated seeds. In the first segregating generation, 64.6% of sib plants shared the same band in PCR amplification using ACMP-490 primer and 66.7% using cnu-316 primer. The uniformity of segregating generations using ACMP-490 and cnu-316 raised in second generation to 68.8%, 70.8%, respectively; in third generation to 93.8%, 100%; in fourth generation to 93.8%, 100%; in fifth generation to 95.8%, 100%; in sixth generation to 100%, 100%. A novel cross was made using selected MS parent and pollen parent. When we checker the horticultural traits using autumn cultivation, the novel cross variety produced H-shaped root comparing other commercial varieties and produced highly uniform radish. Thus we registered this novel cross variety as 'YR ORE' at 2013 (Registration No. 4550).

show abstract

Environmental Risk Assessment of Herbicide Resistant Transgenic Rapeseed (Brassica napus L.) : Responses to <i>Cyprinus carpio</i> fed on herbicide resistant transgenic rapeseed

Oh,

Baek,

Min

et al. 2023

Korean J. Int. Agric.

View full text Add to dashboard Cite

The strategy and current status of Brassica rapa genome project

Cited by 3 publications

References 37 publications

Analysis of the Genetic Diversity of Radish Germplasm through SSR Markers Derived from Chinese Cabbage

Analysis of the Genetic Diversity of Radish Germplasm through SSR Markers Derived from Chinese Cabbage

Development of highly uniform variety for processing using SSR markers in radish (Raphanus sativus L)

Environmental Risk Assessment of Herbicide Resistant Transgenic Rapeseed (Brassica napus L.) : Responses to <i>Cyprinus carpio</i> fed on herbicide resistant transgenic rapeseed

Contact Info

Product

Resources

About