Development of highly uniform variety for processing using SSR markers in radish (Raphanus sativus L)

Abstract

Using commercial radish varieties for processing, about 30% of radish was discarded due to the root shape and low purity. To raise the processing ability, we tried to develop a new variety producing H-shaped root. As another characteristic required in variety for processing is high purity, we tried to raise purity using simple sequence repeats (SSR) markers for testing seed purity in every segregating generation. To develop Male-sterile (MS) seeding parent, we crossed commercial variety of 'Gwan dong spring' and 'Gyeo ryong spring'. One elite inbred was selected as recurrent parent for the MS plant. The major horticultural traits of selected inbred line were disease resistance, late bolting, heat resistance and bright green root top color. To develop pollen parent, we crossed commercial variety of 'Tae sang king' and 'Seoul spring'. We used individual selection method to develop H-shaped hard root and disease resistant inbred. In each segregating generation, we selected one plant based on phenotype and the uniformity of selected plant was tested by SSR markers using self-pollinated seeds. In the first segregating generation, 64.6% of sib plants shared the same band in PCR amplification using ACMP-490 primer and 66.7% using cnu-316 primer. The uniformity of segregating generations using ACMP-490 and cnu-316 raised in second generation to 68.8%, 70.8%, respectively; in third generation to 93.8%, 100%; in fourth generation to 93.8%, 100%; in fifth generation to 95.8%, 100%; in sixth generation to 100%, 100%. A novel cross was made using selected MS parent and pollen parent. When we checker the horticultural traits using autumn cultivation, the novel cross variety produced H-shaped root comparing other commercial varieties and produced highly uniform radish. Thus we registered this novel cross variety as 'YR ORE' at 2013 (Registration No. 4550).

Evaluation of feed value of a by-product of pickled radish for ruminants: analyses of nutrient composition, storage stability, and in vitro ruminal fermentation

“…The use of by-products of food processing has been of great interest in the livestock industry, particularly as it relates to reducing feed-related costs [ 23 ]. A significant amount of BPR is continuously being produced as the market for pickled radish keeps growing in Korea; however, most of it is wasted [ 5 ]. There is therefore solid justification to recycle BPR as a feed source for ruminant animals that have evolutionarily adapted to use low digestible nutrients efficiently in processes facilitated by symbiosis with rumen microbes [ 24 ].…”

“…Many by-products are generated from the manufacture of pickled radish in Korea. By-products of pickled radish (BPR) comprise approximately 300 g/kg of raw material and are generally wasted [ 5 ]. The market for pickled radish has grown rapidly and total production is estimated to be about 40,000 metric tons annually [ 5 ].…”

“…By-products of pickled radish (BPR) comprise approximately 300 g/kg of raw material and are generally wasted [ 5 ]. The market for pickled radish has grown rapidly and total production is estimated to be about 40,000 metric tons annually [ 5 ]. One manufacturer of pickled radish reportedly lost about 300 million Korean won in wasted BPR within a single year.…”