The Standardization of Hemoglobin Measurement

Drabkin, David L.

doi:10.1097/00000441-194801000-00017

Cited by 151 publications

(89 citation statements)

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“…Foram feitas determinações sanguíneas de hematócrito (Goldenfarb et al 1971), hemoglobina (Drabkin 1948) e contagem do número de células vermelhas (Lima et al 1969). A partir desses valores sanguíneos foram calculados (Lima et al 1969): volume corpuscular médio (VCM), hemoglobina corpuscular média (HCM) e concentração de hemoglobina corpuscular média (CHCM).…”

Section: Methodsunclassified

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

et al. 2011

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ResumoO tambaqui é a principal espécie de peixe cultivado na Amazônia Ocidental. Porém durante o processo produtivo, práticas de manejo são necessárias para o monitoramento do crescimento e estado geral da sanidade dos animais. Para isso os animais devem ser anestesiados para maior segurança no trabalho. O eugenol, componente majoritário do óleo de cravo, tem sido bastante utilizado como anestésico alternativo para peixes por ser um produto natural e de baixo custo. Entretanto, estudos tratando de respostas metabólicas em peixes tropicais expostos a diferentes anestésicos são ainda necessários. Dentro desse intuito, o presente trabalho avaliou respostas metabólicas, detectadas por meio de alterações de parâmetros sanguíneos e plasmáticos do tambaqui, exposto ao eugenol em banhos anestésicos simulados. Respostas típicas ao estresse foram detectadas devido ao manuseio imposto aos peixes durante a realização dos banhos anestésicos. O eugenol não reduziu totalmente essas reações ao estresse. Por outro lado, esse anestésico não provocou estresse adicional em exposições curtas de 15 min em concentrações próximas a 20 mg L -1 . PalavRas-Chave: Colossoma macropomum, respostas fisiológicas, hematologia. Evaluation of tambaqui metabolic responses to eugenol in anesthetics bathsabstRaCt Tambaqui is the main farmed fish in the Western Amazon. However, in handling this fish has to be anesthetized for safety purposes, usually when evaluating growth and health conditions. Eugenol, the main component of clove oil, has been reported as an alternative fish anesthetic, because it is an inexpensive natural product. However, continued studies are necessary about the metabolic responses of tropical fish to anesthetics. The present work evaluated metabolic responses of tambaqui to eugenol in simulated anesthetic baths, measuring blood and plasma parameters. Typical metabolic stress responses to handling were detected, but they were not totally reduced by eugenol. On the other hand, the anesthetic dissolved in water did not provoke any extra charge of stress during short-term exposures in concentrations of about 20mg L -1 for 15 min.

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Section: Methodsunclassified

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

et al. 2011

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“…Hemoglobin content in isolated tumors was estimated by Drabkin's cyanmethemoglobin method (Drabkin, 1949). Hemoglobin values were calculated using a hemoglobin standard curve prepared using serial dilutions of mouse blood.…”

Section: Analysis Of Hemoglobin Content In Tumorsmentioning

confidence: 99%

PI4KIIα is a novel regulator of tumor growth by its action on angiogenesis and HIF-1α regulation

Zhang

et al. 2010

Oncogene

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“…Whole blood samples were also collected and serum was separated for various biochemical investigations. Hemoglobin concentration in hemolysate was estimated spectrophotometrically at 540 nm with Drabkin's reagent (6). Serum protein was estimated by the method of Lowry et al (7).…”

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Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine

Suke

Ahmed

Pathak

et al. 2008

Braz J Med Biol Res

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The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P < 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P < 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P < 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P < 0.05) showing phosphamidon-induced oxidative stress. Phosphamidon exposure markedly suppressed humoral immune response as assessed by antibody titer to ovalbumin (4.71 ± 0.51 vs 8.00 ± 0.12 -log 2 ; P < 0.05), and cell-mediated immune response as assessed by leukocyte migration inhibition (25.24 ± 1.04 vs 70.8 ± 1.09%; P < 0.05) and macrophage migration inhibition (20.38 ± 0.99 vs 67.16 ± 5.30%; P < 0.05) response. Phosphamidon exposure decreased IFN-γ levels (40.7 ± 3.21 vs 55.84 ± 3.02 pg/ mL; P < 0.05) suggesting a profound effect of phosphamidon on cell-mediated immune response. A phosphamidon-induced increase in TNF-α level (64.19 ± 6.0 vs 23.16 ± 4.0 pg/mL; P < 0.05) suggests a contributory role of immunocytes in oxidative stress. Co-administration of N-acetylcysteine (3.5 mmol/kg, orally) with phosphamidon attenuated the adverse effects of phosphamidon. These findings suggest that oral N-acetylcysteine treatment exerts protective effect and attenuates free radical injury and immune dysfunction caused by subchronic phosphamidon exposure.

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The Standardization of Hemoglobin Measurement

Cited by 151 publications

References 0 publications

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

PI4KIIα is a novel regulator of tumor growth by its action on angiogenesis and HIF-1α regulation

Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine

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