The spleen is an important site of T cell activation during human hepatosplenic schistosomiasis

Martins‐Filho, Olindo Assis; Mello, J.R.C; Correa-Oliveira, Rodrigo

doi:10.1590/s0074-02761998000700023

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…These data suggest an exchange of cells between these two compartments, demonstrating that the peripheral blood reflects the cellularity of this important lymphoid organ in human schistosomiasis. Consistent with this hypothesis, previous studies in our laboratory [15] demonstrated that after splenectomy, there is a significant decrease in the mean percentage of activated T cells in the peripheral blood of schistosomiasis patients. These data also support the use of splenectomy as an immunological intervention to ameliorate the patient's immune profile ± in cases of severe HS disease, restoring some of the peripheral blood populations to normal levels.…”

Section: Discussionsupporting

confidence: 86%

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The Study of T‐Cell Activation in Peripheral Blood and Spleen of Hepatosplenic Patients Suggests an Exchange of Cells Between these Two Compartments in Advanced Human Schistosomiasis Mansoni Infection

et al. 2002

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A histopathological and immunophenotypic study was performed on the spleen of patients with hepatosplenic (HS) schistosomiasis mansoni. Morphological data demonstrated that all HS patients presented features related to Schistosoma mansoni‐induced splenomegaly, such as red pulp congestion and atrophy/hyperplasia of white pulp. The morphological diversity of the white pulp seems to be associated with the expansion of activated CD4+ T‐cell subpopulation. The data obtained suggest that the spleen is an important site for T‐cell activation during severe chronic infection with S. mansoni. In addition, we have compared the cell populations/subpopulations presented in the peripheral blood with that observed in the spleen of patients with HS schistosomiasis mansoni. We observed a significant increase in the percentage of activated CD4+HLA–DR+ and CD8+HLA–DR+ T cells in both the spleen and the peripheral blood of HS patients in comparison with noninfected individuals (NOR). These data suggest an exchange of cells between these two compartments. However, we observed normal expression of the CD28 molecule by CD8+ T cells in the spleen, despite a lower percentage of these cells in the peripheral blood. This finding supports the hypothesis that the decrease in CD28 expression, by CD8+ cells, is an event that takes place outside the spleen during human schistosomiasis infection. The most important conclusion is the fact that the analysis of T‐cell activation in the peripheral blood reflects the major immunological reactivity that occurs in the spleen during human schistosomiasis and that the morphological aspects of the spleen may reflect the functional activity of T cells. The specificities of T cells and the roles they may play in the pathogenesis during chronic schistosomiasis now need to be determined.

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Section: Discussionsupporting

confidence: 86%

“…Martins‐Filho et al . [15] undertook, for the first time, a comparative analysis of cell phenotype in the spleen of HS patients and NOR individuals. These authors did not show any difference in the mean percentage of T‐cell population and subpopulations (both CD4 + and CD8 + ) in the spleen between HS and NOR groups.…”

Section: Discussionmentioning

confidence: 99%

The Study of T‐Cell Activation in Peripheral Blood and Spleen of Hepatosplenic Patients Suggests an Exchange of Cells Between these Two Compartments in Advanced Human Schistosomiasis Mansoni Infection

et al. 2002

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“…The percentage of CD8␣ + cells among naïve mouse splenocytes is around 10% (Shen et al, 2003) and in spleen biopsies of healthy humans it is around 19% (Martins-Filho et al, 1998), which is much higher than in rainbow trout. In the same line, the proportion of CD8␣ + rainbow trout blood leukocytes is extremely low (approximately 0.3%) compared to 25% in human PBL (Norment and Littman, 1988).…”

Section: Discussionmentioning

confidence: 86%

The expression of CD8α discriminates distinct T cell subsets in teleost fish

Takizawa

Dijkstra

Kotterba

et al. 2011

Developmental & Comparative Immunology

147

120

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“…In humans, Oliveira-Prado et al [50] showed a smaller percent of CD4 + and a higher percent of CD8 + cells in peripheral blood from patients with chronic schistosomiasis. Moreover, our group has previously described the putative role of CD8 + T subsets in controlling morbidity during human schistosomiasis, which was evidenced by the increased levels of activated HLA-DR + CD8 + T lymphocytes in patients presenting intestinal clinical form (INT) of the disease and low levels of CD28 + CD8 + cells in hepatosplenic patients [51–53]. In addition, Teixeira-Carvalho et al [54] observed increased percentage of IL-4 + , IL-5 + , and IL-10 + CD8 + lymphocytes following short-term SEA stimulation of whole blood samples in vitro in the INT group.…”

Section: Discussionmentioning

confidence: 99%

Cytokine Pattern of T Lymphocytes in AcuteSchistosomiasis mansoniPatients following Treated Praziquantel Therapy

Silveira-Lemos

Costa-Silva

Silveira

et al. 2013

Journal of Parasitology Research

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Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis.

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The spleen is an important site of T cell activation during human hepatosplenic schistosomiasis

Abstract: We have undertaken a comparative immunephenotypic study of spleen cells from hepatosplenic patients (HS)

Cited by 10 publications

References 14 publications

The Study of T‐Cell Activation in Peripheral Blood and Spleen of Hepatosplenic Patients Suggests an Exchange of Cells Between these Two Compartments in Advanced Human Schistosomiasis Mansoni Infection

The Study of T‐Cell Activation in Peripheral Blood and Spleen of Hepatosplenic Patients Suggests an Exchange of Cells Between these Two Compartments in Advanced Human Schistosomiasis Mansoni Infection

The expression of CD8α discriminates distinct T cell subsets in teleost fish

Cytokine Pattern of T Lymphocytes in AcuteSchistosomiasis mansoniPatients following Treated Praziquantel Therapy

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