The expression of CD8α discriminates distinct T cell subsets in teleost fish

Takizawa, Fumio; Dijkstra, Johannes M.; Kotterba, Paul; Korytář, Tomáš; Kock, Holger; Köllner, Bernd; Jaureguiberry, Beltran; Nakanishi, Teruyuki; Fischer, Uwe

doi:10.1016/j.dci.2011.02.008

Cited by 147 publications

(134 citation statements)

References 83 publications

(88 reference statements)

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“…The anti-trout CD8a (mAb rat IgG; 7 mg/ml) and the anti-trout CCR7 (pAb rabbit IgG; 2 mg/ml) used in this study were characterized previously (34,35). The anti-trout MHC class II b-chain (mAb mouse IgG1; 2 mg/ml) was characterized above.…”

Section: Flow Cytometrymentioning

confidence: 99%

Identification of Teleost Skin CD8α+ Dendritic-like Cells, Representing a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells

Granja¹,

Leal²,

Pignatelli³

et al. 2015

The Journal of Immunology

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Although fish constitute the most ancient animal group in which an acquired immune system is present, the presence of dendritic cells (DCs) in teleosts has been addressed only briefly, and the identification of a specific DC subset in teleosts remained elusive because of the lack of specific Abs. In mice, DCs expressing CD8α+ in lymphoid tissues have the capacity to cross-present extracellular Ags to T cells through MHC I, similarly to tissue-derived CD103+ DCs and the human CD141+ DC population. In the current study, we identified a large and highly complex subpopulation of leukocytes coexpressing MHC class II and CD8α. This CD8α+ MHC II+ DC-like subpopulation constituted ∼1.2% of the total leukocyte population in the skin, showing phenotypical and functional characteristics of semimature DCs that seem to locally regulate mucosal immunity and tolerance in a species lacking lymph nodes. Furthermore, we identified trout homologs for CD141 and CD103 and demonstrated that, in trout, this skin CD8+ DC-like subpopulation expresses both markers. To our knowledge, these results provide the first evidence of a specific DC-like subtype in nonimmune tissue in teleosts and support the hypothesis of a common origin for all mammalian cross-presenting DCs.

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Section: Flow Cytometrymentioning

confidence: 99%

Identification of Teleost Skin CD8α+ Dendritic-like Cells, Representing a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells

Granja¹,

Leal²,

Pignatelli³

et al. 2015

The Journal of Immunology

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“…These data suggested the involvement of trout CTLs that was further evidenced by the up-regulation of TCR and CD8 genes in these sIgM -cells after allogeneic cell immunization. The generation of monoclonal antibodies for rainbow trout CD8 has allowed further characterization of this population (Takizawa et al, 2011). Sorted trout CD8 + lymphocytes showed great expression of perforin or NK-lysin genes (related to the cytotoxic activity, either specific or not), as well as their up-regulation upon stimulation with the T-lymphocyte-mitogen PHA-L.…”

Section: Specific Cytotoxic Cells or Ctlsmentioning

confidence: 99%

“…Finally, other genes related to the cytotoxic activity have received less attention. In this category, the natural killer enhancing factor (NKEF), which increase the cytotoxic activity in humans but its role is unknown in fish, is up-regulated by viral infections and DNA vaccines (Cuesta & Tafalla, 2009;Ordás et al, 2011;Utke et al, 2007) while granulysin, which is secreted together to granzymes and lyses target cells, gene is up-regulated in CD8 + lymphocytes by mitogen stimulation (Takizawa et al, 2011). Further studies are needed to clearly state the gene expression with either innate or specific cytotoxic activity in fish.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

“…First, the NCCRP-1 gene expression was altered after bacterial infection (Reyes-Becerril et al, 2011;Sakata et al, 2005), administration of immunostimulants (Cuesta et al, 2008b(Cuesta et al, , 2008dLazado et al, ;Reyes-Becerril et al, 2008), exposure to contaminants (Cuesta et al, 2008d) or bacterial vaccination (Caipang et al, 2008), depending on the fish species, tissue, time and dose of exposure, and suggests a parallel effect of fish NCC activity. Perforin gene expression is usually up-regulated after immunization of ginbuna crucian carp with tumor cells (Toda et al, 2011a), after PHA-L (Phaseolus vulgaris leucoagglutinin) stimulation of trout CD8 + cells (Takizawa et al, 2011), after VHSV infection of RTS11 cell line (Ordás et al, 2011) and after viral infection or DNA vaccination in rainbow trout (unpublished data), whilst down-regulated after cadmium exposure (Auslander et al, 2008). In a similar fashion, granzyme genes are up-regulated by bacterial vaccination (Caipang et al, 2008), viral infections (VHSV in RTS11 cell line and SVCV in carp) (Huang et al, 2010;Ordás et al, 2011) and viral infection or DNA vaccination (unpublished data).…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

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Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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“…Infiltrating cells (1 × 10 6 cells/ml) were stained using primary antibodies against either IgM (mab 4C10; [29]), CD8␣ [30] and IgT [19]. IgM and CD8␣ staining was carried out simultaneously using TRICOLOR anti-mouse Ig (Caltag) and FITC anti-rat IgG (H + L) (Jackson ImmunoResearch) conjugates, respectively, while IgT + cells were single-stained using an Alexa488 anti-mouse-IgG (Caltag) conjugate.…”

Section: Isolation and Characterization Of Infiltrated B Cells From Mmentioning

confidence: 99%

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Castro¹,

Martinez-Alonso²,

Fischer³

et al. 2014

Vaccine

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a b s t r a c tIn fish, intramuscular (i.m) injection of plasmid DNA encoding viral proteins has proved a highly effective vaccination strategy against some viral pathogens. The efficacy of DNA vaccination in teleost fish is based on the high level of viral antigen expression in muscle cells inducing a strong and long-lasting protection. However, the mechanisms through which this protection is established and effectuated in fish are still not fully understood. Moreover, similarities to mammalian models cannot be established since DNA vaccination in mammals usually induces much weaker responses. In this work, we have focused on the characterization of the immune cells that infiltrate the muscle at the site of DNA injection in vaccinated fish and the chemokines and chemokine receptors that may be involved in their infiltration. We have demonstrated through diverse techniques that B lymphocytes, both IgM + and IgT + cells, represented a major infiltrating cell type in fish vaccinated with a viral haemorrhagic septicaemia virus (VHSV) glycoprotein-encoding DNA vaccine, whereas in control fish injected with an oil adjuvant mainly granulocyte/monocyte-type cells were attracted. Among twelve chemokine genes studied, only CXCL11 L1, CK5B and CK6 mRNA levels were up-regulated in DNA vaccinated fish compared to fish injected with the corresponding vector backbone. Furthermore, the transcription of CXCR3B, a possible receptor for CXCL11 L1 was also significantly up-regulated in vaccinated fish. Finally, experiments performed with recombinant trout CK5B and CK6 and chemokine expression plasmids revealed that these chemokines have chemotactic capacities which might explain the recruitment of B cells to the site of DNA injection. Altogether, our results reveal that there is an early chemokine-related B cell recruitment triggered by i.m. DNA vaccination against VHSV which might play an important role in the initial phase of the immune response.

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The expression of CD8α discriminates distinct T cell subsets in teleost fish

Cited by 147 publications

References 83 publications

Identification of Teleost Skin CD8α+ Dendritic-like Cells, Representing a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells

Identification of Teleost Skin CD8α+ Dendritic-like Cells, Representing a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells

Fighting Virus and Parasites with Fish Cytotoxic Cells

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

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