The Specificity of Hemagglutinating Bean and Seed Extracts (Lectins)

Wiener, A.S.; Moor‐Jankowski, J.; Gordon, Eve B.

doi:10.1159/000230779

Cited by 12 publications

(7 citation statements)

References 4 publications

(5 reference statements)

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“…Hemagglutination study using human blood group A, B and O erythrocytes has validated earlier findings that blood group non-specific nature of this lectin and requirement of complex sugars as its haptens. Although there are reports on human A, B, O blood group non-specific nature of PHA, there are no reports describing the preference among these blood groups (Wiener et al, 1969;Manage et al, 1972). However, current study has shown that PHA has more affinity towards O but not A and B blood groups.…”

Section: Lectin Blot Analysis Of Salivary Glycoproteins By Biotinylatcontrasting

confidence: 57%

Inhibitory activity of salivary glycoproteins on phytohemagglutins (PHA): Possible molecules to enhance nutritional quality of red kidney beans

Chachadi

Nayanegali

Pujari

et al. 2018

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Food allergy caused by red kidney bean (Phaseolus vulgaris L.) is of serious health concern and is mainly due to its phytohemagglutinins (PHA) content. PHA can enter the circulation after oral uptake and cause IgE mediated allergy. However, studies describing enhancement of nutritional quality of red kidney beans by targeting PHA are not reported. This study was carried out to identify, PHA-inhibitory molecules present in saliva secretions. Results describe that PHA can be effectively inhibited by salivary glycoproteins. Fractionation of salivary proteins by ammonium sulphate precipitation revealed that, PHA-inhibitory proteins can be specifically precipitated at 30-60% of ammonium sulphate saturation. Gel filtration chromatography and lectin-blot analysis of 30-60% ammonium sulphate fraction suggest that only high molecular weight glycoproteins can act as potent inhibitors of PHA. In conclusion, human saliva secretions contain inhibitory glycoproteins which can be used to inhibit PHA effectively. If these glycoproteins are purified to homogeneity, can be used as potent food supplements in order to neutralize allergic potential of PHA, thus increasing the nutritional value of red kidney beans.

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Section: Lectin Blot Analysis Of Salivary Glycoproteins By Biotinylatcontrasting

confidence: 57%

Inhibitory activity of salivary glycoproteins on phytohemagglutins (PHA): Possible molecules to enhance nutritional quality of red kidney beans

Chachadi

Nayanegali

Pujari

et al. 2018

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“…Free of heteroagglutinins are various seed extracts (lectins) bestowed with type-specific hemagglutinating activity. The anti-A reagent made of saline extracts of lima beans (Phaseolus vulgaris) was used for A-B-0 typing of chimpanzee blood [102]. Another reagent of anti-A specificity, free of nonspecific heteroagglutinins, thus suitable for blood grouping primate red cells, was obtained from the snails Helix pomatia [ 5 5 ] and Achatina 5 unijuga Le-used for M-N typing ofchimpanzees and other apes [ 1021.…”

Section: Reagentsmentioning

confidence: 99%

Red cell polymorphisms in nonhuman primates: A review

Socha

Blancher

Moor‐Jankowski

1995

J of Medical Primatology

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“…A 3rd variable are the inhibitors; we have seen in a previous paper [1] that more than 1 sugar may inhibit an agglutinator and 1 or more of these may be present in the glycoproteins of the biological materials tested. Wiener et al [8] have already shown that lectins can be fractionated into reagents with different specificities and the same may be possible with other AbLS.…”

Section: Discussionmentioning

confidence: 93%

Haemagglutination and Haemagglutination-Inhibition Reactions of Extracts from Snails and Sponges

Khalap¹,

Thompson²,

Gold³

1971

Vox Sanguinis

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Agglutination-inhibition, precipitation and precipitation-inhibition tests have been performed with 2 sponge and 4 snail extracts using as inhibitors a number of biological materials and some glycoproteins and glycolipids. Some human salivas and fluids from ovarian pseudomucinous cysts inhibited, others did not inhibit. Inhibiting power was unrelated to presence of ABH substances. Some snail and seed extracts inhibited haemagglutination by Axinella sp. extract and some snail extracts haemagglutination by Cliona celata extract. Axinella sp. extract precipitated non-haemagglutinating Achatina granulata and Hedleyella falconeri snail extracts; this precipitation was inhibited by the same sugars which also inhibit haemagglutination by Axinella sp. extract.

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The Specificity of Hemagglutinating Bean and Seed Extracts (Lectins)

Cited by 12 publications

References 4 publications

Inhibitory activity of salivary glycoproteins on phytohemagglutins (PHA): Possible molecules to enhance nutritional quality of red kidney beans

Inhibitory activity of salivary glycoproteins on phytohemagglutins (PHA): Possible molecules to enhance nutritional quality of red kidney beans

Red cell polymorphisms in nonhuman primates: A review

Haemagglutination and Haemagglutination-Inhibition Reactions of Extracts from Snails and Sponges

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