The separation of GMP from milk whey using the modified chitosan beads

Li, Cunben; Song, Xinning; Hein, San; Wang, Kean

doi:10.1007/s10450-010-9212-7

Cited by 18 publications

(12 citation statements)

References 17 publications

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“…The MIP MAA were found largely macroporous, with the BET specific surface area and pore volume of ~70 m 2 /g, and 0.31 cm 3 /g, respectively. It has been demonstrated in many studies that the adsorption mechanisms on such macroporous polymeric adsorbents are predominantly chemical adsorption in nature, of which the surface area and pore size offer little insightful information. For example, the MIP synthesized by Zhu et al .…”

Section: Resultsmentioning

confidence: 99%

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Molecularly imprinted polymer microspheres prepared via the two‐step swelling polymerization for the separation of lincomycin

Zhang

Zhong

et al. 2019

J of Applied Polymer Sci

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A molecularly imprinted polymer (MIP) was synthesized via a two-step swelling polymerization method for the purification of lincomycin. Polystyrene microspheres were prepared by the dispersion-polymerization process and used as the substrate. Methacrylic acid was used as the functional monomer, whereas ethylene glycol dimethacrylate was the cross-linker. The MIP was structurally characterized and examined for its separation performance at different conditions (temperature, solvents, etc.). It was found that the MIP possesses the good sphericity, porosity, monodispersity, and a high adsorption capacity of (180 μmol/g) in chloroform solution. Comparison studies showed that the MIP presents a higher capacity than the NIP (non-imprinted polymer) in chloroform solution and a much higher capacity in the practical lincomycin fermentation solution extracts, confirming the underlining mechanisms of the MIP. Scatchard plot revealed two adsorption mechanisms on the MIP, whereas the isotherm is better described by Freundlich equation. The adsorption/elution kinetics demonstrated that the MIP possesses good elution/regeneration capabilities with the elution ratio > 93%.

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Section: Resultsmentioning

confidence: 99%

“…possessed a surface area of ~200 m 2 /g, which is less than the related are of NIP. The macroporous, polymeric adsorbents for protein separation presented a surface area of less than 50 m 2 /g …”

Section: Resultsmentioning

confidence: 99%

Molecularly imprinted polymer microspheres prepared via the two‐step swelling polymerization for the separation of lincomycin

Zhang

Zhong

et al. 2019

J of Applied Polymer Sci

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“…For example, Zhu et al found the surface area of a MIP in the range of ∼200 m 2 ·g –1 , but the related NIP presented a higher surface area than the MIP. Xu et al synthesized chitosan-based adsorbents for adsorption of dyes in aqueous solution with the surface area of ∼50 m 2 ·g –1 , The cross-linked chitosan–cyclodextrin adsorbents for the purification of whey proteins presented a surface area of ∼45 m 2 ·g –1 …”

Section: Resuts and Discussionmentioning

confidence: 99%

Synthesizing Vitamin E Molecularly Imprinted Polymers via Precipitation Polymerization

Zhu

Zhang

et al. 2019

J. Chem. Eng. Data

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Molecularly imprinted polymers (MIPs) were prepared by the precipitation polymerization process, with vitamin E as the template molecule, ethylene glycol dimethacrylate as the cross-linker, and three different functional monomers, respectively. Adsorption experiments were conducted to investigate the performance of each MIP and compared to the nonimprinted polymers (NIPs). It was found that MIPs are superior to NIPs in general, with acrylamide-based MIP showing the best adsorption capacity of 38.8 mg·g–1 and an imprinting factor of 2.6. The optimal imprinting ratio and cross-linking ratio to be 1:5 and 5:1, respectively. The Freundlich equation was found superior to Langmuir to describe the adsorption isotherm data on the MIP/NIP. The adsorption of vitamin E on the MIP was found to be a heterogeneous process and consists of two mechanisms, which were confirmed by the analysis of the thermodynamic properties of the adsorption process.

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“…Nakano et al (2004) reported use of epichlorohydrin-treated chitosan resin to separate GMP from whey. Li et al (2010) prepared β-cyclodextrin immobilized chitosan beads cross-linked with 1, 6-hexamethylene diisocyanate for isolation of GMP from whey. These adsorbents are not suitable for production of food grade GMP as they are prepared using toxic chemicals.…”

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confidence: 99%

Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent

Nakano

Betti

2020

Journal of Dairy Research

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Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food. This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent. Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment. The GMP fraction obtained from each column was analyzed for amino acid composition which reflects the purity of the peptide. Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7. Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B. Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation. Further research is needed to develop an efficient inexpensive method to purify GMP.

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The separation of GMP from milk whey using the modified chitosan beads

Cited by 18 publications

References 17 publications

Molecularly imprinted polymer microspheres prepared via the two‐step swelling polymerization for the separation of lincomycin

Molecularly imprinted polymer microspheres prepared via the two‐step swelling polymerization for the separation of lincomycin

Synthesizing Vitamin E Molecularly Imprinted Polymers via Precipitation Polymerization

Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent

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