The sensitivity to rennin of an acid-soluble fraction of casein

Beeby, R.; Mocquot, G.

doi:10.1016/0005-2795(69)90023-3

Cited by 6 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This band, which corresponds to a mol. wt of 9-10 K, is probably macroglycopeptide (Beeby & Mocquot, 1969) though its high [ 32 P] content is somewhat puzzling. The relatively high percentage of carbohydrate causes abnormal migration and makes the staining very difficult (Mullin & Wolfe, 1974).…”

Section: Sds Gel Electrophoretic Identification Of Newly Synthesized mentioning

confidence: 99%

Hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland

Gertler

Weil

Cohen

1982

Journal of Dairy Research

View full text Add to dashboard Cite

SUMMARYExplants from lactating bovine mammary gland were culturedin vitroin serum-free medium through 1–9 d. Casein synthesis was determined by [32P] incorporation into newly synthesized Ca rennin precipitable fraction. High correlation (γ = 0·98) was found between incorporation of [32P] and [3H]amino acids in explants cultured under different hormonal regimes, thus indicating that post-translational phosphorylation is not a rate-limiting step in casein synthesis.Hormonal effects on casein synthesis were studied by supplementing the incubating medium with insulin (I), prolactin (PRL), cortisol (F), thyroxine (T4) and triiodothyronine (T3). It was found that both PRL and I were required absolutely for maximal synthesis and almost maximal effect was achieved with 50 ng/ml. The effect of F was less clear, but some increase was achieved at the 200–1000 ng/ml range. T4and T3did not affect casein synthesis at a range of 10-11–10-7M while a significant inhibition was observed at 2 × 10-5M. A time-course study of casein synthesis further substantiated the dominant role of PRL in maintenance or even elevation of the initial rate of casein synthesis in the explants, through the first 4 d of incubation.

show abstract

Section: Sds Gel Electrophoretic Identification Of Newly Synthesized mentioning

confidence: 99%