Hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland

Gertler, Arieh; Weil, A Spierer; Cohen, Noa

doi:10.1017/s0022029900022536

Cited by 40 publications

(14 citation statements)

References 33 publications

(37 reference statements)

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“…It is clear that insulin is not only essential for milk protein gene expression, but also stimulates and regulates milk protein synthesis at multiple levels in bovine mammary tissue [41]. Insulin is essential for accumulation of casein mRNA in mouse mammary epithelial cells [15] and bovine lactating mammary gland cultured in vitro [42]. Aoki [43] also showed that stage-specific mRNA expression of milk fat globule membrance glycoproteins including BTN1A1 in mouse mammary epithelial cells was regulated in a similar mechanism to that of CSN2.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

Wang

et al. 2009

PLoS ONE

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BackgroundThe objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro.MethodologyMammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition) was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts and epithelial cells successively grew and extended from the culturing mammary tissue at the third day. Pure epithelial cells were obtained by passages culture.Principal FindingsThe strong positive immunostaining to cytokeratin 18 suggested that the resulting cell line exhibited the specific character of epithelial cells. Epithelial cells cultured in the presence of 10% FBS, supraphysiologic concentrations of insulin, and hydrocortisone maintained a normal diploid chromosome modal number of 2n = 60. Furthermore, they were capable of synthesizing β-casein (CSN2), acetyl-CoA carboxylase-α (ACACA) and butyrophilin (BTN1A1). An important finding was that frozen preservation in a mixture of 90% FBS and 10% DMSO did not influence the growth characteristics, chromosome number, or protein secretion of the isolated epithelial cell line.ConclusionsThe obtained mammary epithelial cell line had normal morphology, growth characteristics, cytogenetic and secretory characteristics, thus, it might represent an useful tool for studying the function of Chinese Holstein dairy cows mammary epithelial cell (CMECs).

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Section: Discussionmentioning

confidence: 99%

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

Wang

et al. 2009

PLoS ONE

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“…Preparation of explants from bovine lactating mammary tissue was carried out as previously described (16), and medium was changed every 24 h. Fat synthesis was determined by measuring incorporation of CH 3 -[ 14 C]OOH into the fatty acid fraction for 3 h after 4 days of culture with a selected hormonal regimen (10). a-Lactalbumin secretion into the medium was determined by RIA (10).…”

Section: Bovine Lactating Mammary Gland Explant Culturementioning

confidence: 99%

Effect of Insulin-Like Growth Factor I on Deoxyribonucleic Acid Synthesis and Galactopoiesis in Bovine Undifferentiated and Lactating Mammary Tissuein Vitro*

et al. 1988

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We have demonstrated that insulin-like growth factor I (IGF-I), at physiological concentrations, is a potent mitogen of bovine undifferentiated mammary epithelial cells cultured in collagen in serum-free medium. Its activity is independent of insulin, although at pharmacological concentrations insulin may substitute for IGF-I. The maximal [3H]thymidine incorporation stimulated by either IGF-I or insulin was only 25-40% of that in medium supplemented with 10% fetal calf serum (FCS) only. Epidermal growth factor (EGF) exhibited low mitogenic activity which was not synergistic with IGF-I in serum-free medium. IGF-I and EGF had low synergistic activity when added separately to 10% FCS-supplemented medium. Strong synergism (100% or more) was observed, however, when both factors were added simultaneously, indicating that their maximum mitogenic effect is dependent on a simultaneous presence of other factors existing in FCS. The galactopoietic effect of IGF-I was tested in organ culture of bovine lactating mammary gland. Neither fatty acid synthesis nor alpha-lactalbumin secretion was stimulated by IGF-I, even at 2000 ng/ml. These results indicate that, at least in our in vitro system, galactopoiesis is not affected by IGF-I.

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“…More recently, we have also demonstrated that these hor¬ mones are necessary for casein synthesis (Gertler, Weil & Cohen, 1982), a-lactalbumin secretion and fat syn¬ thesis (Gertler, Cohen & Maoz, 1983) in expiants from bovine lactating mammary glands. Progesterone, on the other hand, inhibits expression of the casein gene in vivo in ovariectomized 14-day pregnant rats (Rosen, Matusik, Richards et al 1980) and in pseudopregnant rabbits (Teyssot & Houdebine, 1981).…”

Section: Introductionmentioning

confidence: 79%

Inhibition of casein and fat synthesis and α-lactalbumin secretion by progesterone in explants from bovine lactating mammary glands

Shamay¹,

Zeelon²,

Ghez³

et al. 1987

Journal of Endocrinology

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The effect of progesterone on casein and fat synthesis, on alpha-lactalbumin secretion and on accumulation of casein mRNA was studied in explants from bovine lactating mammary glands, cultured in the presence of insulin, cortisol or prolactin. All four biological activities were inhibited down to or below the level obtained in medium without prolactin. However, inhibition was only achieved by using concentrations of progesterone exceeding by 1000-fold those required for similar effects in tissue from non-lactating animals. A similar inhibitory effect was also found using high levels of other steroids without 11 beta-hydroxyl, such as deoxycorticosterone and dehydroepiandrosterone, and to some extent with oestradiol-17 beta, but not with cortisol or corticosterone. The inhibition by progesterone did not result from a decrease in explant viability, as determined by glucose uptake.

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Hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland

Cited by 40 publications

References 33 publications

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

Effect of Insulin-Like Growth Factor I on Deoxyribonucleic Acid Synthesis and Galactopoiesis in Bovine Undifferentiated and Lactating Mammary Tissuein Vitro*

Inhibition of casein and fat synthesis and α-lactalbumin secretion by progesterone in explants from bovine lactating mammary glands

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