The isolation and properties of whole casein: Some implications for comparative studies

Graham, E.R.B.; McKenzie, H.A.; Murphy, William H.

doi:10.1016/0305-0491(93)90208-m

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…The lipid‐light phase was concentrated to 350 g L −1 , recovering 93% of the LPO in the heavy phase. 16 L of 25% v/v acetic acid was added to the LPO‐rich stream over a 5‐min period to reduce the pH to 4.3 to precipitate the casein, which represents the majority of total milk protein 21 while LPO remained soluble. The precipitate was aged for 30 min in a low‐shear environment to yield compact, stable particles for the subsequent disk stack centrifuge operated with automated intermittent solids discharge.…”

Section: Methodsmentioning

confidence: 99%

Integrated use of ultra scale‐down and financial modeling to identify optimal conditions for the precipitation and centrifugal recovery of milk proteins

Chhatre

Pampel

Titchener‐Hooker

2011

Biotechnology Progress

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This article investigates the integrated application of ultra scale-down (USD) techniques and economic modeling as a means for identifying optimal bioprocess operating conditions. The benefits of the approach are illustrated for the recovery of lactoperoxidase (LPO) from bovine milk. In the process, milk is skimmed to deplete its lipid content, before being subjected to low pH incubation with acetic acid in order to precipitate the primary impurity (casein). Following removal of the solids by disk stack centrifugation, pH adjustment and filtration, cation exchange chromatography is used as a positive mode column step to bind the LPO before it is polished and freeze dried. An economic model of this process was used to identify where greatest product loss occurs and hence where the largest opportunity cost was being incurred. Scale-down analysis was used to characterize the influence of the critical steps, identified as precipitation and centrifugation, upon LPO recovery. A mathematical model was used to relate the centrifuge feed flowrate and discharge interval to the supernatant yield, and it was shown that increasing the centrifugal solids residence time achieved superior solids de-watering and so higher product yield, although this also increased the overall processing time. To resolve this conflict, scale-down data were used again in conjunction with an economic model to determine the most suitable conditions that maximized annual profit and minimized operating costs. The results demonstrate the power of combining USD data with models of economic and process performance in order to establish the best overall operating strategies for biopharmaceutical manufacture.

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Section: Methodsmentioning

confidence: 99%

Integrated use of ultra scale‐down and financial modeling to identify optimal conditions for the precipitation and centrifugal recovery of milk proteins

Chhatre

Pampel

Titchener‐Hooker

2011

Biotechnology Progress

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Suitability of Animals' Purified Milk Caseins and Their Subunit Κ−caseins as Substrates for Subtilisin and Trypsin

Doğru

Baysal

Aytekin

2001

Preparative Biochemistry and Biotechnology

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Acid casein and kappa-casein were purified from different species of animal's milk, such as cow, sheep, goat, and water buffalo. These caseins were used as substrates for commercially available subtilisin and trypsin. It was established that, when acid caseins were used as a substrate for subtilisin, cow acid casein was found to be a better substrate for the enzymes, compared to other animals' milk casein. It was suggested that this acid casein has significantly more aromatic amino acids, as compared to arginine and lysine. K(M) and Vmax values, which were obtained for cow kappa-casein, showed that cow kappa-casein was a better susbstrate for trypsin than the others, suggesting that cow kappa-casein has a rich content of lysine, arginine, and aromatic amino acids by comparison with the others. The calculated C/N ratio also supports this suggestion.

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The isolation and properties of whole casein: Some implications for comparative studies

Cited by 2 publications

References 38 publications

Integrated use of ultra scale‐down and financial modeling to identify optimal conditions for the precipitation and centrifugal recovery of milk proteins

Integrated use of ultra scale‐down and financial modeling to identify optimal conditions for the precipitation and centrifugal recovery of milk proteins

Suitability of Animals' Purified Milk Caseins and Their Subunit Κ−caseins as Substrates for Subtilisin and Trypsin

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