The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility

Zhang, Sihan; Zhao, Guanglei; Ma, Wei; Song, Yanghui; Huang, Cheng; Xie, Chong; Chen, Kebing; Li, Xiaofeng

doi:10.1016/j.carbpol.2022.119255

Cited by 13 publications

(7 citation statements)

References 60 publications

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“…Figure A shows the FTIR spectra of pADM, HTCC-pADM, and OHTCC-pADM, the typical absorption peaks of collagen were shown in each sample, including amide I, II, III, and amide A and B bands. The absorption peak of the amide I band of collagen at 1655 cm –1 is mainly attributed to CO telescopic vibration in collagen molecules . The absorption peak of collagen amide II band appeared at 1545 cm –1 , which was mainly caused by the synergy of the bending vibration of N–H and the telescopic vibration of C–N, and the amide I and II bands of collagen were directly related to the α helix, β fold, and irregular curl structure in its secondary structure .…”

Section: Results and Discussionmentioning

confidence: 96%

“…Chitosan quaternary ammonium salt is a typical cationic polymer, while the surface of the bacteria is mostly negatively charged proteins, lipopolysaccharides. 17 It is generally believed that the antibacterial mechanism of chitosan quaternary ammonium salt is electrostatic interaction with the bacterial surface to destroy the cell wall, resulting in potassium ions and other cytoplasmic components leakage from the inside of the cell membrane to the outside, thereby hindering the growth and reproduction of bacteria, the electrostatic interaction will also affect the permeability of the cell wall, leading to an imbalance 35 The absorption peak of collagen amide II band appeared at 1545 cm −1 , which was mainly caused by the synergy of the bending vibration of N−H and the telescopic vibration of C−N, and the amide I and II bands of collagen were directly related to the α helix, β fold, and irregular curl structure in its secondary structure. 36 The absorption peak at around 1240 cm −1 is the amide III band, which represents the C−N telescopic vibration in the amide bond, the in-plane bending vibration of N−H, and the absorption peak generated by the swaying vibration of the glycine backbone and the proline side chain -CH 2 group.…”

Section: Characterization Of Ohtccmentioning

confidence: 99%

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Converting Acellular Dermal Matrix into On-Demand Versatile Skin Scaffolds by a Balanceable Crosslinking Approach for Integrated Infected Wounds Therapy

et al. 2023

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Ideal tissue-engineered skin scaffolds should possess integrated therapeutic effects and multifunctionality, such as broad-spectrum antibacterial properties, adjustable mechanical properties, and bionic structure. Acellular dermal matrix (ADM) has been broadly used in many surgical applications as an alternative treatment to the “gold standard” tissue transplantation. However, insufficient broad-spectrum antibacterial and mechanical properties for therapeutic efficacy limit the practical clinical applications of ADM. Herein, a balanceable crosslinking approach based on oxidized 2-hydroxypropyltrimethyl ammonium chloride chitosan (OHTCC) was developed for converting ADM into on-demand versatile skin scaffolds for integrated infected wounds therapy. Comprehensive experiments show that different oxidation degrees of OHTCC have significative influences on the specific origins of OHTCC-crosslinked ADM scaffolds (OHTCC-ADM). OHTCC with an oxidation degree of about 13% could prosperously balance the physiochemical properties, antibacterial functionality, and cytocompatibility of the OHTCC-ADM scaffolds. Owing to the natural features and comprehensive crosslinking effects, the proposed OHTCC-ADM scaffolds possessed the desirable multifunctional properties, including adjustable mechanical, degradable characteristics, and thermal stability. In vitro/in vivo biostudies indicated that OHTCC-ADM scaffolds own well-pleasing broad-spectrum antibacterial performances and play effectively therapeutic roles in treating infection, inhibiting inflammation, promoting angiogenesis, and promoting collagen deposition to enhance the infected wound healing. This study proposes a facile balanceable crosslinking approach for the design of ADM-based versatile skin scaffolds for integrated infected wounds therapy.

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Section: Results and Discussionmentioning

confidence: 96%

Section: Characterization Of Ohtccmentioning

confidence: 99%

Converting Acellular Dermal Matrix into On-Demand Versatile Skin Scaffolds by a Balanceable Crosslinking Approach for Integrated Infected Wounds Therapy

et al. 2023

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“…The glutaraldehyde formed covalent bonds with amino groups of collagen protein through Schiff base reaction or with hydroxyl groups of collagens and glycosaminoglycans to produce a more rigid molecular network, resulting in better mechanical strength of GA-ADM. (Islam et al, 2021;Shin et al, 2021). Additionally, the mechanical strength of the material increases with the porosity decrease (Wang et al, 2016;Wang et al, 2021;Zhang et al, 2022). Thus, the improvement of mechanical strength of GA-ADM partly contributed to porosity decrease.…”

Section: Analysis Of Physicochemical Propertiesmentioning

confidence: 95%

A Comparative Study on Two Types of Porcine Acellular Dermal Matrix Sponges Prepared by Thermal Crosslinking and Thermal-Glutaraldehyde Crosslinking Matrix Microparticles

Huang

Ding

Pan³

et al. 2022

Front. Bioeng. Biotechnol.

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Common commercial porcine acellular dermal matrix (PADM) products take the form of a thin membrane. Given its dense structure, delaying vascularization after implantation remains an issue to be solved. In addition, overlaying multiple sheets to address deep wounds and large tissue defects that are difficult to repair by self-tissues could hinder tissue ingrowth, angiogenesis, and integration. Here, we creatively prepared PADM microparticles through a homogenizing treatment and crosslinked them to ADM sponges by thermal crosslinking (VT-ADM) and thermal-glutaraldehyde crosslinking (GA-ADM). The resulting VT-ADM was thicker than GA-ADM, and both maintained the natural dermal matrix microstructure and thermal stability. The porosity of GA-ADM (mean 82%) was lower than that of VT-ADM (mean 90.2%), but the mechanical strength and hydrophilicity were significantly higher. The two types of ADM sponges showed no obvious difference in cell adhesion and proliferation without cytotoxicity. Furthermore, the human adipose stem cells were co-cultured with ADM sponges which promoted proliferation, tube formation, and migration of endothelial cells, and the GA-ADM group exhibited better migration behavior. There were no markable differences among expressions of pro-angiogenesis genes, including vascular endothelial growth factor, insulin-like growth factor-1, and epidermal growth factor. In a nude mouse model, the VT-ADM and GA-ADM pre-cultured with human adipose stem cells for 1 week in advance were implanted subcutaneously. The VT-ADM and the GA-ADM showed great histocompatibility without local redness, swelling, or necrosis. The vascular density of the local skin flap above the material was visualized using indocyanine green and showed no statistical difference between the two groups. The collagen tissue deposition in the pores and vessel formation within the sponges increased with time. Although VT-ADM had a higher degradation rate in vivo, the integrity of the two scaffolds was preserved. Collectively, the VT-ADM and the GA-ADM retained a natural matrix structure and presented biocompatibility. Thus, the above-mentioned two crosslinking methods for ADM sponges are safe and practicable. The novel ADM sponges with good physicochemical and biological properties are no longer limited to membrane tissue regeneration but could also realize structure remodeling where they act as scaffolds for a soft tissue filler and three-dimensional reconstruction of the tissue with strength requirements.

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“…Cellular Counting Kits 64,65,71,75,101,104,105,115,118,120,134,140,148,152,164,167,170 With respect to mineralization of the material, this is often the least considered and evaluated aspect of in vitro studies, present in only around 36.2% of publications. The most common techniques for this involve mineral staining using either Alizarin Red or Von Kossa but a few studies have also shown promising results using quantitative calcium assays.…”

Section: 5%mentioning

confidence: 99%

The Diamond Concept Enigma: Recent Trends of Its Implementation in Cross-linked Chitosan-Based Scaffolds for Bone Tissue Engineering

Agnes

Karoichan

Tabrizian

2023

ACS Appl. Bio Mater.

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An increasing number of publications over the past ten years have focused on the development of chitosan-based cross-linked scaffolds to regenerate bone tissue. The design of biomaterials for bone tissue engineering applications relies heavily on the ideals set forth by a polytherapy approach called the “Diamond Concept”. This methodology takes into consideration the mechanical environment, scaffold properties, osteogenic and angiogenic potential of cells, and benefits of osteoinductive mediator encapsulation. The following review presents a comprehensive summarization of recent trends in chitosan-based cross-linked scaffold development within the scope of the Diamond Concept, particularly for nonload-bearing bone repair. A standardized methodology for material characterization, along with assessment of in vitro and in vivo potential for bone regeneration, is presented based on approaches in the literature, and future directions of the field are discussed.

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The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility

Cited by 13 publications

References 60 publications

Converting Acellular Dermal Matrix into On-Demand Versatile Skin Scaffolds by a Balanceable Crosslinking Approach for Integrated Infected Wounds Therapy

Converting Acellular Dermal Matrix into On-Demand Versatile Skin Scaffolds by a Balanceable Crosslinking Approach for Integrated Infected Wounds Therapy

A Comparative Study on Two Types of Porcine Acellular Dermal Matrix Sponges Prepared by Thermal Crosslinking and Thermal-Glutaraldehyde Crosslinking Matrix Microparticles

The Diamond Concept Enigma: Recent Trends of Its Implementation in Cross-linked Chitosan-Based Scaffolds for Bone Tissue Engineering

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