The role of the bla KPC gene in antimicrobial resistance of Klebsiella pneumoniae

Abstract

Background and Objectives: Klebsiella pneumoniae isolates that produce K. pneumoniae carbapenemase (KPC) have be- come a grave concern for the treatment of infections. KPC-producing strains are not only able to hydrolyze carbapenems but are also resistant to a variety of β-lactam and non-β-lactam antibiotics. The present study evaluated the prevalence of bla in K. pneumoniae infections and determined the antimicrobial susceptibility of the isolates. Materials and Methods: The K. pneumoniae isolates were identified by biochemical tests and confirmed by genotyping. The modified Hodge test (MHT) was performed to detect carbapenemases, and antimicrobial susceptibility was determined for all isolates by the disc diffusion method. Also, for MHT-positive isolates, supposed to carbapenemases isolates, broth microdilution method was used to measure the minimum inhibitory concentrations (MICs) of meropenem and colistin. Results: The bla KPC genotypic evaluation revealed that only 5 of 96 isolates carried bla genes. Antimicrobial pattern showed that isolates carrying bla were resistant to cefepime, ticarcillin/tazobactam, and aztreonam discs. Also, results of broth microdilution method showed that KPC-producing K. pneumoniae was resistant to meropenem and colistin, according to the CLSI and EUCAST. Conclusion: In this study nearly half the isolates showed carbapenemase activity as shown by MHT results, but only few of them were carrying bla . Thus bla gene is not the main cause of resistance spread to carbapenems in Isfahan, Iran.

The evaluation of Human papilloma virus and human herpes viruses (EBV, CMV, VZV HSV‐1 and HSV‐2) in semen samples

“…Although many studies have attempted to evaluate the prevalence of different infectious risk factors, the prevalence of viral ones in sperm specimen is not clear. It is supposed that viral infections can accelerate the progression of different kinds of diseases, for example, previous studies have reported that HPV E6 is able to downregulate the expression of p53 (Abbasi et al., 2020; Foresta et al., 2013; Ghasemnejad et al., 2019; Hemmat & Baghi, 2018; Hu et al., 2011). Furthermore, there are numerous relationships between Herpesviridae, such as EBV, and different kinds of health problems, especially gastric and breast cancer (Aris et al., 2020; Golrokh et al., 2020).…”

Depletion of ARGs in antibiotic-resistance Klebsiella, Pseudomonas and Staphylococcus in hospital urines by electro and photo-electro disinfection

“…In this work, bla KPC , bla OXA-50 and mecA genes were selected as ARG models for K. pneumoniae, P. aeruginosa and S. aureus, respectively. The K. pneumoniae strains harboring the bla KPC gene represent the most common cause of carbapenem resistance in Enterobacteriaceae [53]. Carbapenemases hydrolyze carbapenem antibiotics and other β-lactam antibiotics provide a high antibiotic resistance [54].…”

Detection of Enterovirus, Herpes Simplex, Varicella Zoster, Epstein‐Barr and Cytomegalovirus in cerebrospinal fluid in meningitis patients in Iran

“…Statistics have shown that meningitis is responsible for 0.6% of all deaths, and survivors usually suffer from various types of disabilities 19 . There are a number of tight relationships between different risk factors and central nervous system disorders, but the role of viral infections has drawn much attention 13,20‐22 . As symptoms of this disease are nonspecific, molecular analysis is essential to identify causative agents 23,24 .…”

“… 19 There are a number of tight relationships between different risk factors and central nervous system disorders, but the role of viral infections has drawn much attention. 13 , 20 , 21 , 22 As symptoms of this disease are nonspecific, molecular analysis is essential to identify causative agents. 23 , 24 In this study, the prevalence of Herpesviridae and Picornaviridae was investigated in CSF of patients, suffering from meningitis.…”