The role of TGF-β1 and cytokines in the modulation of liver fibrosis by Sho-saiko-to in rat's bile duct ligated model

Chen, Ming-Ho; Chen, Jung-Chou; Tsai, Chi‐Cheng; Wang, Wen-Chuang; Chang, Deching; Tu, Dom‐Gene; Hsieh, He-Yen

doi:10.1016/j.jep.2004.09.040

Cited by 48 publications

(44 citation statements)

References 27 publications

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“…Thus, TGF- β 1 has been considered as a therapeutic target in DN and other chronic kidney diseases [29]. XCHT has been documented to inhibit hepatic expression of TGF- β 1 mRNA and protein in many experimental fibrogenic animal models [30, 31]. The in vivo results from the present study also indicated that XCHT treatment could decrease TGF- β 1, fibronectin, and collagen IV expression in the kidney of STZ-diabetic mice.…”

Section: Discussionsupporting

confidence: 65%

Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

Lin

Yen

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Xiao-Chai-Hu-Tang (XCHT), a traditional Chinese medicine formula consisting of seven medicinal plants, is used in the treatment of various diseases. We show here that XCHT could protect type-1 diabetic mice against diabetic nephropathy, using streptozotocin (STZ)-induced diabetic mice and high-glucose (HG)-exposed rat mesangial cell (RMC) as models. Following 4 weeks of oral administration with XCHT, renal functions and renal hypertrophy significantly improved in the STZ-diabetic mice, while serum glucose was only moderately reduced compared to vehicle treatment. Treatment with XCHT in the STZ-diabetic mice and HG-exposed RMC resulted in a decrease in expression levels of TGF-β1, fibronectin, and collagen IV, with concomitant increase in BMP-7 expression. Data from DPPH assay, DHE stain, and CM-H2DCFDA analysis indicated that XCHT could scavenge free radicals and inhibit high-glucose-induced ROS in RMCs. Taken together, these results suggest that treatment with XCHT can improve renal functions in STZ-diabetic mice, an effect that is potentially mediated through decreasing oxidative stress and production of TGF-β1, fibronectin, and collagen IV in the kidney during development of diabetic nephropathy. XCHT, therefore merits further investigation for application to improve renal functions in diabetic disorders.

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Section: Discussionsupporting

confidence: 65%

Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

Lin

Yen

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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“…Some experimental studies reported partial success with some substances such as melatonin [3], pegylated interferon [4], malotilate [5], halofuginone [6], and Sho-saiko-to [7], a Far East herbal therapeutic, in this setting. An effective therapeutic strategy against the development of hepatic fibrosis is still needed.…”

mentioning

confidence: 99%

The Effects of N-Acetylcysteine on Bile Duct Ligation–Induced Liver Fibrosis in Rats

Tahan

Tarçın²,

Tahan

et al. 2007

Dig Dis Sci

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Stellate cells are activated by free radicals, and synthesize collagen. N-acetylcysteine (NAC) is a precursor of reduced glutathione and a potent scavenger of hydroxyl radicals and has potential antifibrotic effects. We aimed to test the effects of NAC on bile duct ligation (BDL) induced liver damage in rats. Forty-seven Wistar rats were divided into 5 groups: group 1, BDL+NAC (n=10); group 2, BDL (n=10); group 3, sham+NAC (n=10); group 4, sham (n=10); and group 5, control group (n=10). NAC (50 micromol/kg per day) or saline of single doses were administered intraperitoneally for 28 days. Serum biochemical and liver oxidative stress parameters were studied. Liver collagen level was determined by the method of Lopez de Leon and Rojkind. Liver slides were stained by hematoxylin and eosin and Masson trichrome\Gomory reticulum staining. Aspartate aminotransferase (AST) and alkaline phosphatase levels in the BDL+NAC group were lower than the BDL group and were higher than the control groups (all P< .001). Malondialdehyde, luminal, and glutathione levels in group 1 were lower than the BDL group (P= .01, P= .002, and P< .001) and higher than the control groups (all P< .001). NAC had no effect on alanine aminotransferase (ALT), gammaglutamyl transferase, bilirubin, albumin, or lucigenin levels. Liver collagen levels were higher in the BDL groups (P< .001); however, NAC had no effect on the collagen levels. The BDL groups showed stage 3 fibrosis; all the control groups were normal. NAC improved some biochemical parameters (AST, alkaline phosphatase) and oxidative stress parameters (malondialdehyde, luminol, glutathione) in the BDL model. NAC was found to be effective on cholestasis-induced hepatotoxicity. However, NAC was inefficient as an antifibrotic agent within a 1-month period of administration in the BDL model.

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“…In an animal model of cirrhosis, SST significantly reduced the serum fibrotic marker PIII NP, partly by down-regulating transforming growth factor (TGF)-beta1 and platelet-derived growth factor (PDGF) expression. It also suppressed the increasing tendency of IL-1beta and stimulated the production of TNF-alpha to inhibit Ito cell proliferation and collagen formation (Chen et al, 2005). SST potentiates IFN-gamma, IL-4 responses upon anti-CD3 stimulation (Kang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

A single arm phase II study of a Far-Eastern traditional herbal formulation (sho-sai-ko-to or xiao-chai-hu-tang) in chronic hepatitis C patients

Deng

Kurtz

Vickers

et al. 2011

Journal of Ethnopharmacology

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The role of TGF-β1 and cytokines in the modulation of liver fibrosis by Sho-saiko-to in rat's bile duct ligated model

Cited by 48 publications

References 27 publications

Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

The Effects of N-Acetylcysteine on Bile Duct Ligation–Induced Liver Fibrosis in Rats

A single arm phase II study of a Far-Eastern traditional herbal formulation (sho-sai-ko-to or xiao-chai-hu-tang) in chronic hepatitis C patients

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