The Role of Sulfide Anion in the Development of Oxidative Stress in the Liver Under Conditions of Chronic Alcoholic Hepatitis

Mykytenko, A. О.; Akimov, O. Ye.; Yeroshenko, G. А.; Непорада, Каріне Степанівна

doi:10.26724/2079-8334-2022-3-81-223-226

Cited by 4 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The experiments were performed on 24 male Wistar rats weighing 180-220 g. The animals were divided into 4 groups (6 animals in each): І -control group; II -group animals, which received phenformin hydrochloride (phenformin, Sigma-Aldrich), as an activator of AMP-activated protein kinase, orally at a dose of 10 mg/kg [12] daily for 63 days (AMPK activation group); ІІI group -animals, on which we simulated alcoholic hepatitis (n = 6) by forced intermittent alcoholization for 5 days, with repetition after two days by intraperitoneal administration of 16.5% ethanol solution in 5% glucose solution, at the rate of 4 ml/kg body weight. Then, they were converted to 10% ethanol as the only source of drinking water, first proposed by Yu.…”

Section: Animalsmentioning

confidence: 99%

Phenformin attenuates the oxidative-nitrosative stress in the liver of rats under long-term ethanol administration

Mykytenko,

Akimov,

Yeroshenko

et al. 2024

Ukr.Biochem.J.

View full text Add to dashboard Cite

Modulation of the AMP-activated protein kinase (AMPK) pathway activity is considered to be a promising option in the development of approaches to chronic alcoholic hepatitis treatment. Phenformin, which is a biguanide, has been reported to increase AMPK activity. The aim of this work was to estimate the effect of phenformin as AMPK activator on the development of oxidative-nitrosative stress in the liver of rats under conditions of long-term ethanol administration. The experiments were performed on 24 male Wistar rats, divided into 4 groups: control; animals, which received phenformin hydrochloride orally at a dose of 10 mg/kg daily for 63 days; animals with a forced intermittent alcoholization for 5 days by intraperitoneal administration of 16.5% ethanol solution in 5% glucose at the rate of 4 ml/kg b.w. and subsequent transfer to 10% ethanol as the only source of drinking; animals with chronic alcohol hepatitis simulation and phenformin administration. Superoxide dismutase, catalase, NO synthase isoforms activity, superoxide anion radical production, concentration of malonic dialdehyde, peroxynitrite, nitrites, nitrosothiols concentration and oxidative modification of proteins (OMP) were estimated in liver homogenates. The increased production of oxygen and nitrogen active forms and OMP intensification in the liver of rats under long-term administration of ethanol was detected. Phenformin introduction under long-term ethanol administration was shown to limit the excess peroxynitrite formation and to prevent oxidative damage to rat liver proteins. Keywords: AMP-activated protein kinase., chronic alcoholic hepatitis, liver, oxidative and nitrosative stress, phenformin

show abstract

Section: Animalsmentioning

confidence: 99%

Phenformin attenuates the oxidative-nitrosative stress in the liver of rats under long-term ethanol administration

Mykytenko,

Akimov,

Yeroshenko

et al. 2024

Ukr.Biochem.J.

View full text Add to dashboard Cite

show abstract

Influence of Doxorubicin on the Development of Oxidative-Nitrosative Stress in the Liver of Rats Under Conditions of Chronic Alcoholic Hepatitis

Mykytenko,

Akimov,

Neporada

2024

MCCh

View full text Add to dashboard Cite

Introduction. Chronic alcohol consumption leads to oxidative-nitrosative liver injury, which induces the release of cytokines and growth factors, leading to activation of hepatic stellate cells and fibrosis. Modern studies have revealed a close connection between AMP-activated protein kinase and fibrogenesis. The aim of the study – to determine the effect of inhibition of AMP-activated protein kinase by the administration of doxorubicin on the development of oxidative-nitrosative stress in the liver of rats under conditions of long-term administration of ethanol. Research Methods. Experiments were performed on 24 white, sexually mature male Wistar rats, weighing 180-220 g. Chronic alcoholic hepatitis was modeled by the method of forced intermittent alcoholization according to Yu.M. Stepanov (2017). Doxorubicin was administered at a dose of 1.25 mg/kg IV 4 times a week throughout the experiment, which lasted 63 days. The activity of NO-synthase isoforms, the concentration of nitrite and peroxynitrite, the activity of arginase, superoxide dismutase and catalase, the concentration of malondialdehyde, oxidation-modified proteins, nitrosothiols and sulfide anion, and the production of superoxide anion were determined in the homogenate of the liver of rats. The significance of the differences was assessed by the Mann-Whitney U-test at p<0.05. Results and Discussion. Administration of doxorubicin under the conditions of chronic alcoholic hepatitis modeling reduced the activity of the inducible isoform of NO-synthase by 4 times, the activity of superoxide dismutase by 1.95 times and increased the activity of catalase by 1.77 times in the liver of rats compared to chronic alcoholic hepatitis. Under these conditions, the concentration of malonic dialdehyde in the liver increased by 1.71 times, the production of superoxide anion radical increased by 1.3 times, the concentration of peroxynitrite increased by 1.9 times, sulfide anion decreased by 2.11 times, while OMP in the liver of rats decreased by 1.98 times compared to chronic alcoholic hepatitis. Conclusion. Administration of doxorubicin against the background of chronic alcoholic hepatitis limits the oxidative modification of liver proteins and the production of nitric oxide from the inducible isoform of NO-synthase.

show abstract

Biochemical Changes in the Extracellular Matrix of Rat Liver During Chronic Alcohol Intoxication

Mykytenko¹,

Akimov²,

Yeroshenko³

et al. 2022

WOMAB

View full text Add to dashboard Cite

The Role of Sulfide Anion in the Development of Oxidative Stress in the Liver Under Conditions of Chronic Alcoholic Hepatitis

Cited by 4 publications

References 12 publications

Phenformin attenuates the oxidative-nitrosative stress in the liver of rats under long-term ethanol administration

Phenformin attenuates the oxidative-nitrosative stress in the liver of rats under long-term ethanol administration

Influence of Doxorubicin on the Development of Oxidative-Nitrosative Stress in the Liver of Rats Under Conditions of Chronic Alcoholic Hepatitis

Biochemical Changes in the Extracellular Matrix of Rat Liver During Chronic Alcohol Intoxication

Contact Info

Product

Resources

About