The role of nitric oxide (NO) in the human internal anal sphincter

Tomita, Ryouichi; Tanjoh, Katsuhisa; Fujisaki, Shigeru; Fukuzawa, Masahiro

doi:10.1007/s005350170082

Cited by 30 publications

(29 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies have shown the dense innervation of the feline and human pyloric sphincter by using immunohistochemistry for neuropeptides (Edin et al 1980a, b;Domoto et al 1992) and nNOS (Altdorfer et al 1996;Feher et al 2001;Tomita et al 1999). A study on guinea-pig pylorus (Cai and Gabella 1984) has shown that the density of innervation, i.e.…”

Section: Discussionmentioning

confidence: 96%

Unique distribution of interstitial cells of Cajal in the feline pylorus

et al. 2007

View full text Add to dashboard Cite

The feline gastrointestinal (GI) tract is an important model for GI physiology but no immunohistochemical assessment of interstitial cells of Cajal (ICC) has been performed because of the lack of suitable antibodies. The aim of the present study was to investigate the various types of ICC and associated nerve structures in the pyloric sphincter region, by using immunohistochemistry and electron microscopy to complement functional studies. In the sphincter, ICC associated with Auerbach's plexus (ICC-AP) were markedly decreased within a region of 6-8 mm in length, thereby forming an interruption in this network of ICC-AP, which is otherwise continuous from corpus to distal ileum. In contrast, intramuscular ICC (ICC-IM) were abundant within the pylorus, especially at the inner edge of the circular muscle adjacent to the submucosa. Similar distribution patterns of nerves positive for vesicular acetylcholine transporter (VAChT), nitric oxide synthase (NOS) and substance P (SP) were encountered. Quantification showed a significantly higher number of ICC-IM and the various types of nerves in the pylorus compared with the circular muscle layers in the adjacent antrum and duodenum. Electron-microscopic studies demonstrated that ICC-IM were closely associated with enteric nerves through synapse-like junctions and with smooth muscle cells through gap junctions. Thus, for the first time, immunohistochemical studies have been successful in documenting the unique distribution of ICC in the feline pylorus. A lack of ICC-AP guarantees the distinct properties of antral and duodenal pacemaker activities. ICC-IM are associated with enteric nerves, which are concentrated in the inner portion of the circular muscle layer, being part of a unique innervation pattern of the sphincter.

show abstract

Section: Discussionmentioning

confidence: 96%

Unique distribution of interstitial cells of Cajal in the feline pylorus

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Neurogenic relaxation and IJP were reportedly mediated by the neurotransmitter NO in the gastric fundus and pyloric sphincters from the rat (Boeckxstaens et al, 1991a;McLaren et al, 1993;Jenkinson et al, 1995;Curro et al, 1996;Curro and Preziosi, 1998;De Man et al, 1998;Lefebvre, 1998), guinea pig (Kojima et al, 1993), dog (Bayguinov et al, 1999), pig (Colpaert and Lefebvre, 2000), mouse (Selemidis and Cocks, 2000;Suzuki et al, 2000;Ergun and Ogulener, 2001), Japanese monkey (N. Toda, K. Ayajiki, and T. Okamura, unpublished data), and human (Tomita et al, 1999;Tonini et al, 2000). Typical responses to nitrergic nerve stimulation of the isolated monkey pyloric sphincter are shown in Fig.…”

Section: B Stomachmentioning

confidence: 99%

Gastrointestinal Function Regulation by Nitrergic Efferent Nerves

Toda

Herman

2005

Pharmacol Rev

View full text Add to dashboard Cite

“…17,18 NO, in addition to adrenergic and cholinergic neurotransmission, plays a major role in mediating anorectal motility 2,14 and NO is involved in the inhibitory response of the IAS to nonadrenergic noncholinergic nerve stimulation. 9,[19][20][21][22] However, the role of NO in smooth muscle relaxation in the rectum is unclear.…”

Section: Introductionmentioning

confidence: 99%