This study is aimed to develop biology's learning media in the form of Adobe Flash based on HOTS for eleventh graders of senior high school. This study employs research and development (R & D) as method developed by Thiagarajan with a four D model that is modified with three stages, viz: define, design, and development. Products have been developed then validated by experts in their fields and have been revised. Based on the results of media validation conducted by experts, the validation value is 3.03, meanwhile the materials validation by the expert is 3.42, further the language validation by the expert is 3.03. Validation results obtained from media experts, materials, languages and teachers considered to prove that biology's learning media developed are valid for used in the learning process and for HOTS questions there were some suggestions from experts to add the number of questions to help students get used to HOTS questions