The role of endothelial dysfunction in the pathogenesis of impaired diabetic wound healing: A novel therapeutic target?

Hanson, R.; Chan, Fuan Chiang; Bouchier-Hayes, D.

doi:10.1016/j.mehy.2007.02.040

Cited by 19 publications

(23 citation statements)

References 29 publications

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“…Experimental and clinical wound healing studies show that the diabetic state is accompanied by delayed or decreased repair capacity at almost all stages of wound healing including cellular migration, cellular proliferation, capillary growth and metabolic activity within the granulation tissue [7,13]. Mechanisms by which these effects are brought about remain to be established [14]. Given the significant results of the DCCT and the UKPDS and previous work by Andreassen et al [5,6,15], one would expect upregulation of all wound parameters following treatment with insulin.…”

Section: Discussionmentioning

confidence: 99%

Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model

et al. 2010

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“…Pathologic oxidative stress conditions, such as diabetes and cardiovascular disease, potentiate endothelial dysfunction eventually leading to ischemic vascular damage and become impediment to wound healing. Current therapeutic strategies focusing on protection of endothelial oxidative damage, accelerating endothelial wound healing, or promoting angiogenesis may have a role in diabetic and oxidative related vascular disease [2]. …”

Section: Introductionmentioning

confidence: 99%

Phyllanthus emblicaL. Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting

Chularojmontri

Suwatronnakorn

Wattanapitayakul

2013

Evidence-Based Complementary and Alternative Medicine

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Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 µmole/mg. PE at 0.1 to 10 µg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency.

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“…Reviews have been published on the role of NO in wound healing, and a most recent one also reviews the development of various NO donors . In addition, the condition of diabetes has been documented as having a link with NO deficiencies in animals and in humans …”

mentioning

confidence: 99%

Nitric oxide levels in wound fluid may reflect the healing trajectory

Bernatchez

Menon²,

Stoffel³

et al. 2013

Wound Repair Regeneration

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We analyzed nitric oxide metabolites (nitrate and nitrite, NOx) and other biomarkers in human wound fluids and correlated these markers with wound healing status (progressing or worsening) based on patient's wound history. Samples were collected pre- and postcleansing from patients with wounds of various etiologies and analyzed for NOx, matrix metalloproteinase activity, and elastase activity. A laboratory method was developed to analyze NOx which can detect at least 5 μM in samples as small as 10 μL. A nitrate-free sample collection device was identified to match the sensitivity of this new assay (most "nitrate-free" products tested contained nitrate levels higher than this detection limit when extracted in such a small volume). The correlation between pre- and postcleansing biomarker values, and the diagnostic potential of the biomarkers to wound progress were analyzed. Fifty wounds provided samples that were suitable for NOx analysis. The pre- and postcleansing values for NOx showed good correlation (r = 0.72); the correlation was not very strong for matrix metalloproteinase and elastase. Data analysis showed that NOx represents the best metabolite to discriminate between worsening and progressing wounds, and suggested that a two cut point diagnostic test using NOx is better than a single cut point test to identify progressing from worsening wounds.

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The role of endothelial dysfunction in the pathogenesis of impaired diabetic wound healing: A novel therapeutic target?

Cited by 19 publications

References 29 publications

Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model

Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model

Phyllanthus emblicaL. Enhances Human Umbilical Vein Endothelial Wound Healing and Sprouting

Nitric oxide levels in wound fluid may reflect the healing trajectory

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