The role of curcumin on intestinal oxidative stress, cell proliferation and apoptosis after ischemia/reperfusion injury in rats

Abstract

The aim of this study was to demonstrate the role of curcumin on oxidative stress, cell proliferation and apoptosis in the rat intestinal mucosa after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ curcumin; each group contain 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the curcumin group, 3 days before I/R, curcumin (100 mg/kg) was administered by gastric gavage. All animals were sacrificed at the end of reperfusion and intestinal tissues samples were obtained for biochemical and histopathological investigation in all groups. Curcumin treatment significantly decreased the elevated tissue malondialdehyde levels and increased of reduced superoxide dismutase, and glutathione peroxidase enzyme activities in intestinal tissues samples. I/R caused severe histopathological injury including mucosal erosions and villous congestion and hemorrhage. Curcumin treatment significantly attenuated the severity of intestinal I/R injury, with inhibiting of I/R-induced apoptosis and cell proliferation. These results suggest that curcumin treatment has a protective effect against intestinal damage induced by intestinal I/R. This protective effect is possibly due to its ability to inhibit I/R-induced oxidative stress, apoptosis and cell proliferation.

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

“…It is generally accepted that ischemic heart injury is a pathological process, which will cause extensive death of cells (Yucel et al, 2011). Furthermore, ischemic heart diseases can produce apoptosis, and the apoptosis might be related to both extrinsic and intrinsic apoptotic pathways.…”

Curcumin inhibits autophagy and apoptosis in hypoxia/reoxygenation-induced myocytes

“…Previous studies have demonstrated that curcumin attenuates I/R injury in various organs in vivo and in vitro, including the intestines, retina, liver and renal system, through anti-oxidative stress and anti-apoptotic mechanisms (20)(21)(22)(23) through the attenuation of oxidative stress and mitochondrial dysfunction. However, the beneficial effects of curcumin on the extent of apoptosis and autophagy in myocardial I/R, and their potential mechanisms, remain to be elucidated.…”

Neuroprotective effect of curcumin on spinal cord in rabbit model with ischemia/reperfusion

“…Curcumin, a herbal medicine with powerful antioxidant, 17 has been widely used as a therapeutic agent for a variety of diseases from cancer to Alzheimer's to infectious diseases, 18 especially for I/R related injury in different tissues. 6,[19][20][21] For example, just recently, Avci et al…”

“…Recently, it was reported that administration of curcumin could ameliorate I/R injury in rat kidney, myocardium, and nervous tissue. [6][7][8] However, to the best of our knowledge, little is known about its effect on I/R injured spinal cord. In this study, we examined its neuro-protective effect and potential mechanism in a rabbit model with I/R injury.…”