The role of biomechanical forces and MALAT1/miR‐329‐5p/PRIP signalling on glucocorticoid‐induced osteonecrosis of the femoral head

Li, Guomin; Li, Bing; Li, Bo; Zhao, Jie; Wang, Xiaoquan; Luo, Rui; Li, Yankun; Li, Jun; Hu, Ruyin

doi:10.1111/jcmm.16510

Cited by 6 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the expression level of miRNAs also infected by its upstream molecular lncRNA, which was another noncoding RNA molecule of a transcript greater than 200 bp in length that were no or little translational potential [ 48 ]. LncRNAs act as miRNA sponge, contained miRNA response elements and could bind to miRNA, which would prevent miRNA from inhibiting target genes and upregulate corresponding gene expression [ 49 , 50 ]. Due to their essential role in cell information regulation, noncoding RNA molecule served as a circulating disease diagnostic marker, which received widespread attention as new molecular markers [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

IRF8 and its related molecules as potential diagnostic biomarkers or therapeutic candidates and immune cell infiltration characteristics in steroid-induced osteonecrosis of the femoral head

Liang

Liu

et al. 2023

J Orthop Surg Res

View full text Add to dashboard Cite

Purpose Steroid-induced osteonecrosis of the femoral head (SONFH) was a refractory orthopedic hip joint disease in the young and middle-aged people, but the pathogenesis of SONFH remained unclear. We aimed to identify the potential genes and screen potential therapeutic compounds for SONFH. Methods The microarray was obtained for blood tissue from the GEO database, and then it identifies differentially expressed genes (DEGs). The DEGs were analyzed to obtain the differences in immune cell infiltration. The gene functional enrichment analysis of SONFH was analyzed. The PPI of DEGs was identified through the STRING database, and the cluster modules and hub genes were ascertained using MCODE and CytoHubba, and the ROC curve of hub genes was analyzed, and the tissues distribution of hub genes was understood by the HPA, Bgee and BioGPS databases. The hub genes and target miRNAs and corresponding upstream lncRNAs were predicted by TargetScan, miRDB and ENCORI database. Subsequently, we used CMap, DGIdb and L1000FWD databases to identify several potential therapeutic molecular compounds for SONFH. Finally, the AutoDockTools Vina, PyMOL and Discovery Studio were employed for molecular docking analyses between compounds and hub genes. Results The microarray dataset GSE123568 was obtained related to SONFH. There were 372 DEGs including 197 upregulated genes and 175 downregulated genes by adjusted P value < 0.01 and |log2FC|> 1. Several significant GSEA enrichment analysis and biological processes and KEGG pathway associated with SONFH were identified, which were significantly related to cytoskeleton organization, nucleobase-containing compound catabolic process, NOD-like receptor signaling pathway, MAPK signaling pathway, FoxO signaling pathway, neutrophil-mediated immunity, neutrophil degranulation and neutrophil activation involved in immune response. Activated T cells CD4 memory, B cells naïve, B cells memory, T cells CD8 and T cells gamma delta might be involved in the occurrence and development of SONFH. Three cluster modules were identified in the PPI network, and eleven hub genes including FPR2, LILRB2, MNDA, CCR1, IRF8, TYROBP, TLR1, HCK, TLR8, TLR2 and CCR2 were identified by Cytohubba, which were differed in bone marrow, adipose tissue and blood, and which had good diagnostic performance in SONFH. We identified IRF8 and 10 target miRNAs that was utilized including Targetsan, miRDB and ENCORI databases and 8 corresponding upstream lncRNAs that was revealed by ENCORI database. IRF8 was detected with consistent expression by qRT-PCR. Based on the CMap, DGIdb and L1000FWD databases, the 11 small molecular compounds that were most strongly therapeutic correlated with SONFH were estradiol, genistein, domperidone, lovastatin, myricetin, fenbufen, rosiglitazone, sirolimus, phenformin, vorinostat and vinblastine. All of 11 small molecules had good binding affinity with the IRF8 in molecular docking. Conclusion The occurrence of SONFH was associated with a “multi-target” and “multi-pathway” pattern, especially related to immunity, and IRF8 and its noncoding RNA were closely related to the development of SONFH. The CMap, DGIdb and L1000FWD databases could be effectively used in a systematic manner to predict potential drugs for the prevention and treatment of SONFH. However, additional clinical and experimental research is warranted.

show abstract

Section: Discussionmentioning

confidence: 99%

IRF8 and its related molecules as potential diagnostic biomarkers or therapeutic candidates and immune cell infiltration characteristics in steroid-induced osteonecrosis of the femoral head

Liang

Liu

et al. 2023

J Orthop Surg Res

View full text Add to dashboard Cite

show abstract

“…Our research results indicate that a hip flexion angle of 110° exhibited greater necrotic area deformation and equivalent stress compared to 60 and 0°. The necrotic area in ONFH is typically located in regions of concentrated stress, and excessive hip joint loading accelerates disease progression 30–32 . The greater equivalent stress with larger hip flexion angles suggests a more disrupted mechanical environment within the acetabulum, which is detrimental to healing and regenerative repair of the necrotic area.…”

Section: Discussionmentioning

confidence: 99%

“…The necrotic area in ONFH is typically located in regions of concentrated stress, and excessive hip joint loading accelerates disease progression. 30 , 31 , 32 The greater equivalent stress with larger hip flexion angles suggests a more disrupted mechanical environment within the acetabulum, which is detrimental to healing and regenerative repair of the necrotic area. From a classification perspective, the L type showed less favorable results compared to other typing metrics, exhibiting a higher risk of collapse.…”

Section: Discussionmentioning

confidence: 99%

The Effect of the Hip Flexion Angle in Osteonecrosis of the Femoral Head Based on China‐Japan Friendship Hospital Classification ‐ A Finite Element Study

Wen,

Liang,

Luo

et al. 2023

Orthopaedic Surgery

View full text Add to dashboard Cite

ObjectiveThe alteration in the mechanical environment of the necrotic area is the primary cause of the collapse observed in osteonecrosis of the femoral head (ONFH). This study aims to evaluate the biomechanical implications of the China‐Japan Friendship Hospital (CJFH) classification system and hip flexion angles on the necrotic area in ONFH using finite element analysis (FEA). The goal is to provide valuable guidance for hip preservation treatments and serve as a reference for clinical diagnosis and therapeutic interventions.MethodsHip tomography CT scan data from a healthy volunteer was used to create a 3D model of the left hip. The model was preprocessed and imported into Solidworks 2018, based on the CJFH classification. Material parameters and boundary conditions were applied to each fractal model in ANSYS 21.0. Von Mises stresses were calculated, and maximum deformation values were obtained to evaluate the biomechanical effects of the load on the necrotic area and post‐necrotic femur, as well as assess each fractal model's collapse risk.Results(1) At the same hip flexion angle, maximum deformation followed this order: M Type < C Type < L Type. The L3 type necrotic area experienced the most significant deformation at 0, 60, and 110° angles (1.121, 1.7913, and 1.8239 mm respectively). (2) Under the same CJFH classification, maximum deformation values increased with hip flexion angle (0 < 60 < 110°), suggesting a higher risk of collapse at larger angles. (3) Von Mises stress results showed that the maximum stress was not located in the necrotic area but near the inner and outer edge of the femoral neck, indicating decreased stiffness and strength of the subchondral bone after osteonecrosis.ConclusionThe study found that femoral head collapse risk was higher when the necrotic area was located in the lateral column under the same stress load and flexion angle. Mechanical properties of the necrotic area changed, resulting in decreased bone strength and stiffness. Large‐angle hip flexion is more likely to cause excessive deformation of the necrotic area; thus, ONFH patients should reduce or avoid large‐angle hip flexion during weight‐bearing training in rehabilitation activities.

show abstract

LncRNAs in Kawasaki disease and Henoch-Schönlein purpura: mechanisms and clinical applications

Yang,

Tang,

et al. 2023

Mol Cell Biochem

View full text Add to dashboard Cite

The role of biomechanical forces and MALAT1/miR‐329‐5p/PRIP signalling on glucocorticoid‐induced osteonecrosis of the femoral head

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 31 publications

IRF8 and its related molecules as potential diagnostic biomarkers or therapeutic candidates and immune cell infiltration characteristics in steroid-induced osteonecrosis of the femoral head

IRF8 and its related molecules as potential diagnostic biomarkers or therapeutic candidates and immune cell infiltration characteristics in steroid-induced osteonecrosis of the femoral head

The Effect of the Hip Flexion Angle in Osteonecrosis of the Femoral Head Based on China‐Japan Friendship Hospital Classification ‐ A Finite Element Study

LncRNAs in Kawasaki disease and Henoch-Schönlein purpura: mechanisms and clinical applications

Contact Info

Product

Resources

About