The role of activated charcoal in plant tissue culture

Thomas, T. Dennis

doi:10.1016/j.biotechadv.2008.08.003

Cited by 380 publications

(277 citation statements)

References 218 publications

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“…It is suggested that calli need a certain amount of picloram to form somatic embryos and activated charcoal is important in absorbing the excess. Activated charcoal has also been reported to help in somatic embryo induction, maturation, or germination by absorbing toxic metabolites in several crop species (Thomas 2008). In Ocotea catharinensis, a forest tree species, somatic embryo induction occurred only on MS basal medium supplemented with 0.03 g/l activated charcoal in the dark (Moura-Costa et al 1993).…”

Section: Effect Of Activated Charcoal On Indirect Somatic Embryo Indumentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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Section: Effect Of Activated Charcoal On Indirect Somatic Embryo Indumentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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“…Activated charcoal at 83.3-833 mM is often incorporated into the root induction media, including for E. camaldulensis, E. globulus, E. grandis, E. grandis × E. urophylla, E. regnans F.Muell., and E. saligna [14,[82][83][84]88,[139][140][141][142][143][144]. Activated charcoal may act by adsorbing inhibitory compounds, decreasing phenolic oxidation, altering medium pH, or reducing irradiance at the base of the shoot [145,146].…”

Section: Adventitious Root Formationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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“…The often-inhibitory effect on growth, observed for treatments with activated charcoal, may be due to the adsorbent property of activated charcoal so that acting as both growth promoter and inhibitor. This is attributed to an excellent pore network with a great internal surface of the activated charcoal, wherein several culture-medium inhibitory substances or toxic products released by explants can remain adsorbed (CHAGAS et al, 2005;THOMAS, 2008). As a result, this compound adsorbs not only toxic compounds but also essential nutrients to plant growth, which are released slowly (GUSON; MORAES; RONCONI, 2012; VILLA; PASQUAL; SILVA, 2014).…”

Section: Figure 1 Protocorms Of Oeceoclades Maculata (Lindl) With Lmentioning

confidence: 99%

PYROLIGNEOUS LIQUOR EFFECT ON IN AND EX VITRO PRODUTION OF Oeceoclades maculata (Lindl). Lindl.1

et al. 2017

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-Pyroligneous fraction from wood carbonization can be used for several purposes. In this sense, this study aimed to test a pyroligneous liquor extracted during carbonization of forest residues for the vegetative development of the orchid Oeceoclades maculata. The concentrations of pyroligneous liquor used in the treatments were T1 = 0 mL L . These treatments were tested together with and without activated charcoal for in vitro and ex vitro propagation. In vitro developed protocorms were assessed according to development or not, the presence of fungus, leaves, and roots. For ex vitro evaluations, leaf size, size, the number of roots, total plant height, and the number of buds were assessed. Possible differences between treatments were tested by means of analysis of variance (ANOVA) and Tukey's test at 5% significance. The use of pyroligneous liquor at low doses was significant in vegetative tissue development as well as for root and leaf formations in O. maculata seedlings since it presents all the essential mineral elements to the structural development of these plants. Keywords:Orchid. Vegetative propagation. Pyroligneous acid. USO DO LICOR PIROLENHOSO NA PRODUÇÃO DE MUDAS IN VITRO E EX VITRO DEOeceoclades maculata (Lindl). Lindl.RESUMO -A fração pirolenhosa obtida com a carbonização da madeira pode ser utilizada para diversos fins. Sendo assim, este trabalho teve por objetivo testar o licor pirolenhoso extraído durante o processo de carbonização de resíduos florestais no desenvolvimento vegetativo da orquídea Oeceoclades maculata. As concentrações do licor pirolenhoso utilizadas nos tratamentos foram: T1 = 0 ml.L -1 , T2 = 1 ml.L -1 , T3 = 2 ml.L -1 , T4 = 3 ml.L -1 , T5 = 4 ml.L -1 e T6 = 5 ml.L -1 , esses tratamentos foram testas com e sem carvão ativado no desenvolvimento in vitro e ex vitro da orquídea. As seguintes características dos protocórmos desenvolvidos in vitro foram avaliadas: com fungo, sem desenvolvimento, em desenvolvimento, presença de folhas e raízes. Para o desenvolvimento ex vitro foram avaliados: Tamanho das folhas, tamanho e número de raízes, altura total da planta, quantidade de gemas. Possíveis diferenças entre os tratamentos foram testada por meio de Análise de variância (ANOVA) e teste de Tukey a 5% de significância. O uso do licor pirolenhoso em dosagem pequenas mostrou-se significativo no desenvolvimento dos tecidos vegetativos e na formação radicular e foliar de Oeceoclades maculata, uma vez que apresenta todos os elementos minerais essenciais ao desenvolvimento estrutural da planta.Palavras-Chave: Orquídea. Propagação vegetativa. Ácido pirolenhoso.

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The role of activated charcoal in plant tissue culture

Cited by 380 publications

References 218 publications

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Tissue Culture of Corymbia and Eucalyptus

PYROLIGNEOUS LIQUOR EFFECT ON IN AND EX VITRO PRODUTION OF Oeceoclades maculata (Lindl). Lindl.1

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