The role of 5-HT7 receptors on isoproterenol-induced myocardial infarction in rats with high-fat diet exacerbated coronary endothelial dysfunction

Çınar, İrfan; Halıcı, Zekai; Dinçer, Büşra; Şirin, Büşra; Çadırcı, Elif

doi:10.1177/0960327120916821

Cited by 13 publications

(12 citation statements)

References 57 publications

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“…5). The current findings are in covenant with the results observed by Cinar et al (2020) who found that IL-6 mRNA expression and 5-HT7 receptor mRNA expression levels increased in MI control. Elevation in the mRNA expression of 5-HT7 receptor and inflammatory cytokines (IL-6 and IL-1β) in the present research may be attributed to endothelial dysfunction due to damage of vascular tissues, through elevation of plasma TG, low-density lipoprotein, and Ox-LDL in the MI group as shown in Figure 3.…”

Section: Discussionsupporting

confidence: 91%

“…The reagent reaction mixture (25 µl) consisted of 12.5 µl of SYBR Green master mix (Thermo-Fisher ® Invitrogen TM ), cDNA template (1 µl), and primer pairs (0.25 µM). The sequence of primer pairs was obtained from Khan et al (2013) and Cinar et al (2020) for interleukin-6 (IL-6), IL-1β, and 5-hydroxytryptamine-7 receptor (5-HT7) (Table 1). Real-time PCR was performed with a Rotor-Gene ® MDx instrument programmed at 2 minutes at 50°C followed by 10 minutes at 95°C, 45 cycles (95°C for 20 seconds, 60°C for 30 seconds, and 72°C for 30 seconds), melting curve program (60°C-95°C).…”

Section: Gene Expression Analysis Of Il-6 Il-1β and 5ht-7mentioning

confidence: 99%

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Cardioprotective potency of anthocyanin-rich extract of red cabbage against isoproterenol-induced myocardial infarction in experimental animals

Mohamed¹,

Mabrok²,

Sherein³

et al. 2021

J Appl Pharm Sci

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The current research was realized for the cardioprotective effect of anthocyanin-rich extract of red cabbage (ARERC) on isoproterenol-induced (ISO) myocardial infarction (MI) in rats. Total phenolic, anthocyanin content, and antioxidant activity were assessed in ARERC. ARERC acute toxicity was evaluated. The results revealed that ARERC contains a high amount of total phenolic (633.9 mg gallic acid equivalents/100 g) and total anthocyanin (618.2 mg Cy3G/100 g) in association with antioxidant activity against (1,1-diphenyl-2-picrylhydrazyl) radicals and possesses high reducing capacity. ARERC was completely safe up to 12 g/kg mice BW. ISO-induced myocardial injury was recognized by elevation in relative heart weight, plasma cardiac marker enzymes (lactate dehydrogenase, creatine kinase, aspartate transaminase, and alanine transaminase), oxidative stress markers (malondialdehyde, superoxide dismutase, and catalase), inflammatory marker C-reactive protein, and histological changes in the cardiac tissue. ARERC oral administration (100 and 200 mg/kg) before MI initiation diminished oxidative stress and inflammation, reinstated the cardiac antioxidants, and improved all changes in cardiac tissue in rats. ISO increased inflammatory cytokines expression (IL-6 and IL-1) and 5-hydroxytryptamine-7 receptor in the cardiac tissue. Results suggested that administration of ARERC alleviates inflammation, stress of oxidation, and damage in the cardiac tissues. Results suggested the beneficial effect of ARERC as an alternative treatment against cardiovascular diseases, especially MI.

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Section: Discussionsupporting

confidence: 91%

Section: Gene Expression Analysis Of Il-6 Il-1β and 5ht-7mentioning

confidence: 99%

Cardioprotective potency of anthocyanin-rich extract of red cabbage against isoproterenol-induced myocardial infarction in experimental animals

Mohamed¹,

Mabrok²,

Sherein³

et al. 2021

J Appl Pharm Sci

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“…Total RNA was isolated from homogenized lung tissues following the instructions provided for the RNeasy Mini Kit (QIAGEN). cDNA was synthesized by reverse transcription of complementary DNA from isolated RNA samples according to the instructions of the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA), as previously described [ 29 ]. The sequences of PCR primer pairs used for each gene are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS

et al. 2022

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Background Acute respiratory distress syndrome (ARDS) is a life-threatening disease caused by the induction of inflammatory cytokines and chemokines in the lungs. There is a dearth of drug applications that can be used to prevent cytokine storms in ARDS treatment. This study was designed to investigate the effects of tocilizumab and dexamethasone on oxidative stress, antioxidant parameters, and cytokine storms in acute lung injury caused by oleic acid in rats. Methods Adult male rats were divided into five groups: the CN (healthy rats, n = 6), OA (oleic acid administration, n = 6), OA + TCZ-2 (oleic acid and tocilizumab at 2 mg/kg, n = 6), OA + TCZ-4 (oleic acid and tocilizumab at 4 mg/kg, n = 6), and OA + DEX-10 (oleic acid and dexamethasone at 10 mg/kg, n = 6) groups. All animals were euthanized after treatment for histopathological, immunohistochemical, biochemical, PCR, and SEM analyses. Results Expressions of TNF-α, IL-1β, IL-6, and IL-8 cytokines in rats with acute lung injury induced by oleic acid were downregulated in the TCZ and DEX groups compared to the OA group (P < 0.05). The MDA level in lung tissues was statistically lower in the OA + TCZ-4 group compared to the OA group. It was further determined that SOD, GSH, and CAT levels were decreased in the OA group and increased in the TCZ and DEX groups (P < 0.05). Histopathological findings such as thickening of the alveoli, hyperemia, and peribronchial cell infiltration were found to be similar when lung tissues of the TCZ and DEX groups were compared to the control group. With SEM imaging of the lung tissues, it was found that the alveolar lining layer had become indistinct in the OA, OA + TCZ-2, and OA + TCZ-4 groups. Conclusions In this model of acute lung injury caused by oleic acid, tocilizumab and dexamethasone were effective in preventing cytokine storms by downregulating the expression of proinflammatory cytokines including TNF-α, IL-1β, IL-6, and IL-8. Against the downregulation of antioxidant parameters such as SOD and GSH in the lung tissues caused by oleic acid, tocilizumab and dexamethasone upregulated them and showed protective effects against cell damage.

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“…The purity and concentration of the isolated RNA and cDNA were determined by a nucleic acid measurement. 24…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

Şengül

Gelen

Yıldırım

et al. 2023

Environmental Toxicology

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Acrylamide (ACR) is used in many fields such as cosmetics, paper, and textile industries. It also occurs at very high temperatures in some foods. Gonadotoxic effects of ACR have been found in experimental animals. Many studies use flavonoids to prevent the reproductive side effects of ACR. Naringin (NA) is a flavonoid and it has been determined by studies that it has no toxic effect on tissues. In our study, we aimed to determine the protective effect of NA against the damage of ACR on testicular tissue and the reproductive system in rats. In our study, 50 Spraque Dawley male rats weighing 220‐250 grams were used. Control: Only intragastric saline was administered for 10 days. ACR: Animals received ACR (40 mg/kg, intraperitoneally) for 10 days. NA50+ACR: Animals were given NA for 10 days and each NA was one hour after the administration of ACR. NA100+ACR: Animals received NA for 10 days and one hour after each NA was given ACR. NA100: Animals were given NA for 10 days. At the end of the applications, the rats were euthanized by cervical dislocation under anesthesia. Serum FSH, LH, and Dihydrotestosterone levels were compared between the groups. In addition, oxidative stress, inflammation, expression of some reproductive enzymes, and apoptosis markers were determined in testicular tissues. When these parameters were compared between groups, ACR induced testicular dysfunction and tissue damage in rats. We determined that only the NA application did not cause tissue damage. and the administration of NA along with ACR significantly reduced ACR‐induced testis toxicity.

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The role of 5-HT7 receptors on isoproterenol-induced myocardial infarction in rats with high-fat diet exacerbated coronary endothelial dysfunction

Cited by 13 publications

References 57 publications

Cardioprotective potency of anthocyanin-rich extract of red cabbage against isoproterenol-induced myocardial infarction in experimental animals

Cardioprotective potency of anthocyanin-rich extract of red cabbage against isoproterenol-induced myocardial infarction in experimental animals

Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS

Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

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