Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

Abstract

Acrylamide (ACR) is used in many fields such as cosmetics, paper, and textile industries. It also occurs at very high temperatures in some foods. Gonadotoxic effects of ACR have been found in experimental animals. Many studies use flavonoids to prevent the reproductive side effects of ACR. Naringin (NA) is a flavonoid and it has been determined by studies that it has no toxic effect on tissues. In our study, we aimed to determine the protective effect of NA against the damage of ACR on testicular tissue and the reproductive system in rats. In our study, 50 Spraque Dawley male rats weighing 220‐250 grams were used. Control: Only intragastric saline was administered for 10 days. ACR: Animals received ACR (40 mg/kg, intraperitoneally) for 10 days. NA50+ACR: Animals were given NA for 10 days and each NA was one hour after the administration of ACR. NA100+ACR: Animals received NA for 10 days and one hour after each NA was given ACR. NA100: Animals were given NA for 10 days. At the end of the applications, the rats were euthanized by cervical dislocation under anesthesia. Serum FSH, LH, and Dihydrotestosterone levels were compared between the groups. In addition, oxidative stress, inflammation, expression of some reproductive enzymes, and apoptosis markers were determined in testicular tissues. When these parameters were compared between groups, ACR induced testicular dysfunction and tissue damage in rats. We determined that only the NA application did not cause tissue damage. and the administration of NA along with ACR significantly reduced ACR‐induced testis toxicity.

Catalpol ameliorates inflammation and oxidative stress via regulating Sirt1 and activating Nrf2/HO‐1 signaling against acute kidney injury

“…Numerous studies have suggested that inflammation and oxidative stress play an important role in the pathophysiology of various diseases, such as lung, testis, liver, thyroid, and kidney toxicity and pulmonary fibrosis 34–44 . Moreover, emerging findings exhibited that Cat has obvious anti‐inflammatory, antioxidant, and antiapoptotic properties and its vital protective function in multiple organ damage 45,46 .…”

“…Hence, it is essential to reveal the pathogenesis of SAKI, thereby paving the way for the develop- pulmonary fibrosis. [34][35][36][37][38][39][40][41][42][43][44] Moreover, emerging findings exhibited that Cat has obvious anti-inflammatory, antioxidant, and antiapoptotic properties and its vital protective function in multiple organ damage. 45,46 For instance, Xia et al manifested that Cat protected the spinal cord from spinal cord injury in mice through mediating microRNA-142-mediated HMGB1/TLR4/NF-κB signaling pathway.…”

Acrylamide-induced meiotic arrest of spermatocytes in adolescent mice by triggering excessive DNA strand breaks: Potential therapeutic effects of resveratrol

“…11 In addition, recent studies have linked increased oxidative stress, inflammation, and apoptosis to AA-induced reproductive toxicity. 40,41 These studies were performed on whole testes and little is known regarding the potential testicular target cells of AA, such as spermatocytes, and their involvement in meiosis and spermatogenesis.…”

Selenium reduces acrylamide‐induced testicular toxicity in rats by regulating HSD17B1, StAR, and CYP17A1 expression, oxidative stress, inflammation, apoptosis, autophagy, and DNA damage

“…for 10 consecutive days. 28,29 Se0.5 + ACR group: Rats received a daily dose of Se (0.5 mg/kg, i.g.) 13 followed by a daily dose of ACR (40 mg/kg) (i.p.)…”

“…Se1 + ACR groups: Rats received a daily dose of Se (1 mg/kg, i. g.) 13 followed by a daily dose of ACR (40 mg/kg, i.p.) 28,29 for 10 consecutive days.…”