The Role Formation of Methylglyoxal, Carbonyl Compound, Hydrogen Peroxide and Advance Oxidation Protein Product Induced Cadmium in Ovarian Rat

Husna, Alvi; Ramadhani, Endira; Eva, D T; Yulita, A. F.; Suhartono, Eko

doi:10.7763/ijcea.2014.v5.402

Cited by 8 publications

(8 citation statements)

References 24 publications

(23 reference statements)

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“…MG was determined by reaction with dinitrophenylhydrazine according to a modified Racker method [41]. We combined 25 μl of blood sample with 350 μl of DNPH (0.1% DNPH in 2N HCl) and then added 2.125 mL of distilled water to each tube.…”

Section: Determining Mgsmentioning

confidence: 99%

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

Yepifantseva¹,

Muravlyova²,

Semenikhina³

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Prolonged exposure to stress is known to change homeostasis and can lead to the development of psychopathological disorders, including depression. Exposure to stress factors contributes to the emergence of reactive oxygen species in the brain. The data characterizing changes of the oxidative metabolism under conditions of chronic stress are insufficient, which determine the relevance of the research in this direction. The determination of reactive carbonyl derivatives of proteins (RCDP), methylglyoxal (MG), and malondialdehyde (MDA) levels can be considered an informative method for studying the dynamics of oxidative metabolism. AIM: The aim of the study was to study the RCDP, MG, and MDA in red blood cells and blood plasma in rats experiencing chronic unpredictable moderate stress (CUMS). METHODS: A total of 20 male outbred rats weighing 450–500 g were used in the study. The animals were divided into two groups of ten rats each using randomized selection. The experimental group of animals was exposed to the impact of diverse stress factors, according to the CUMS model for 21 days. The control group of animals remained in standard conditions and did not undergo CUMS model. Blood sampling was performed twice for all rats the day before the experiment started and on 21st day to determine the level of RCDP, MG, and MDA in their erythrocytes and blood plasma. RESULTS: The study revealed a significant decrease of RCDP (p = 0.007) and MDA levels in erythrocytes (p = 0.013), and MDA level in blood plasma (p = 0.005) of rats of the main experimental group. The control group showed a significant increase of RCDP level in plasma (p = 0.008), a two-fold increase of MDA in erythrocytes (p = 0.05) with a decrease of MDA in plasma (p = 0.011). A tendency for MG increase was noted in erythrocytes of rats in both the main group and the control group. CONCLUSION: The early stages of CUMS were accompanied by the violation of oxidative metabolism with the emergence and accumulation of RCDP and MDA in the blood of animals. The decreased emergence of RCDP and MDA in the later stages can be associated with an excess of oxidized substrates in the blood of animals of the experimental group. The revealed trend toward MG growth in animals of both groups suggests its increased synthesis in erythrocytes, which, in our opinion, should be considered a negative factor affecting the deformability of red cells. In turn, a violation of the deformability of red blood cells leads to a violation of microcapillary blood flow, which contributes to the development of hypoxic damage in different tissues. Thus, the obtained data confirm the different directions of changes in RCDP and MDA levels in the blood of animals experiencing CUMS or acute stress. Our results prove the need for further research on changes in oxidative metabolism in chronic stress disorders.

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Section: Determining Mgsmentioning

confidence: 99%

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

Yepifantseva¹,

Muravlyova²,

Semenikhina³

et al. 2020

Open Access Maced J Med Sci

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“…Thus, the environment is all that exists outside an organization, which consists of: (1) Abiotic (Dead Environment), namely the environment outside an organism consists of objects or natural factors that are not alive. (2) Biotics (Environment), namely the environment outside an organization, which consists of living organisms, such as plants, animals and humans (Husna et al, 2014).…”

Section: Innovation Of Vocational Technology Educationmentioning

confidence: 99%

The Instrumental Framework to Measuring Environmental Awareness

Kencanasari

Surahman

Permana

2019

INVOTEC

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Environmental awareness needs to be increased because it plays an important role for the survival of life. To find out the level of public awareness of the environment it is necessary to conduct a workshop or environmental awareness training. The purpose of this study is to identify and generate an instrument indicator to measure the environmental awareness. This article looks at a variety of relevant literature in order to explore the aspects that must be present In the instrument to measuring the environmental awareness level. The literature study results are obtained the aspects that must have to consist of two aspects namely pro-environment and not pro-environment, in which indicators are made from the start of knowledge, attitudes and behavior. The implication of this literature review is to provide information and recommend a set of a framework to academics in making instruments measuring the level of public awareness of the environment.

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“…AOPPs have fairly pronounced biological properties similar to AGEs and can bind to the receptor for advanced glycation end products (RAGE) that lead to chronic kidney disease development [12]. Of particular interest is methylglyoxal one of the most reactive carbonyl compound, effecting on intracellular signaling systems [13]. One of the enzymes that play an important role in protecting cells against the toxic effects of oxidative stress is catalase (CAT).…”

Section: Introductionmentioning

confidence: 99%

Drug-induced Nephropathy and Molecular Patterns of Oxidative Stress

Lee¹,

Bakirova²,

Muravlyova³

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Drug-induced kidney disorder is a frequent adverse event which contributes to morbidity and even incapacitation. Our current knowledge of drug-induced kidney disease is limited due to varying definitions of kidney injury, incomplete assessment of concurrent risk factors, and lack of long term outcome reporting. AIM: The discovery and development of novel biomarkers and local (renal) response mechanisms, which can diagnose kidney damage earlier and more accurately, are needed for effective prevention of drug-induced nephrotoxicity. METHODS: Forty patients from 22 to 50 years old with drug-induced nephropathy (43 females, 27 males) were included in the study. The determination of oxidative stress modifications as advanced oxidation protein products (AOPPs), protein reactive carbonyl derivatives (PRCD), methylglyoxal, and catalase (CAT) level were assayed. AOPP was rated in blood plasma through the Witko-Sarsat (1996) method, PRCD by Levine (2000) method, methylglyoxal through the Racker (1963) method, CAT was evaluated with Koroljuk (1988) and Aebi (1984) methods. Conditionally, 30 healthy-matched persons were included in the control group. RESULTS: In psychotropic and nonsteroidal anti-inflammatory drugs (NSAID) drug-induced nephropathy patients, there was a tendency to increase the concentration of AOPP in blood plasma, PRCD, and methylglyoxal in erythrocytes relative to the upper limits of normal ranges. Moreover, there was a significant decrease of the CAT level in blood plasma relative to the upper limits of normal ranges. CONCLUSION: Thus, the results of the present study permit us to draw the following conclusions. The patients with psychotropic and NSAIDs drug-induced nephropathy have increased level of oxidative stress products and increased neutrophil gelatinase-associated lipocalin level even in normal eGFR. The mechanisms that lead to the development of oxidative stress and the production of modified proteins are different in patients treated with different drugs.

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The Role Formation of Methylglyoxal, Carbonyl Compound, Hydrogen Peroxide and Advance Oxidation Protein Product Induced Cadmium in Ovarian Rat

Abstract: Abstract-Cadmium (Cd) is a heavy metal that be a source of concern for industrial workers and it was proposed in the formation of advance glycation end products (AGEs) such as methylglyoxal (MG

Cited by 8 publications

References 24 publications

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

The Instrumental Framework to Measuring Environmental Awareness

Drug-induced Nephropathy and Molecular Patterns of Oxidative Stress

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