The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment

Lai, Tongfei; Ying, Wang; Fan, Yaya; Zhou, Yingying; Bao, Ying; Zhou, Ting

doi:10.1016/j.ijfoodmicro.2016.12.017

Cited by 32 publications

(19 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study showed that the deletion of the PatN gene in a P. expansum strain resulted in an 87.5% decrease in PAT production ( Barad et al, 2013 ). According to the RNA-seq results in this study, Cin/Cit created a PAT restrictive condition to P. expansum , so that the expressions of most genes involved in PAT biosynthetic pathway, particularly the early steps, were significantly down-regulated, which were similar to the previous observations ( Tannous et al, 2014 ; Lai et al, 2017 ). Furthermore, the qRT-PCR was performed and demonstrated that the expressions of all the 15 genes were down-regulated under Cin/Cit stress (1/2 MIC) ( Figure 5A ).…”

Section: Resultssupporting

confidence: 87%

“…The agarose gel electrophoresis, a NanoDrop ® 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, United States), a Qubit ® Fluorometer 2.0 (Life Technologies, Carlsbad, CA, United States) and an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, United States) were used to test the concentration and integrity of RNA samples ( Wang H. et al, 2016 ). The cDNA libraries were constructed according to previous reports and were sequenced on the Illumina HiSeq2000 platform (HiSeq2000, Illumina, San Diego, CA, United States, 2010) ( Lin et al, 2013 ; Lai et al, 2017 ). The resulting RNA-seq reads were mapped onto the reference genome of P. expansum Link isolate d1 from Israel (denoted as PEXP) using Tophat v2.0.12 ( Kim et al, 2013 ; Ballester et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

“…P. expansum can spread rapidly under the suitable condition and secretes PAT, a mycotoxin with potential mutagenic, carcinogenic, teratogenic and embryotoxic effects on humans ( Moake et al, 2005 ). Currently, besides certain prophylactic measures applied to prevent the development of pathogen in the storage environments, the control of postharvest pathogens mainly depends on the synthetic fungicides ( Lai et al, 2017 ). However, losses of the fungicides efficiency due to the emergence of fungicide-resistant pathogens, and public concern over chemical residues in food and environment have promoted investigations of alternative strategies to combat fungal decay and to increase food safety and public health ( Tian et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

et al. 2018

View full text Add to dashboard Cite

Penicillium expansum, as a main postharvest pathogen of fruits, can secrete patulin (PAT), causing fruit decay and health problems. In this study, the antifungal test, SEM (scanning electron microscope) observation, transcriptional profile, PAT biosynthesis, and physiological characters of P. expansum exposed to cinnamaldehyde and citral combination (Cin/Cit) were evaluated. Cin/Cit could inhibit the mycelial growth and spore germination of P. expansum in a dose-dependent manner. Besides, Cin/Cit caused spores and mycelia wrinkled and depressed by SEM observation. Gene expression profiles of P. expansum were conducted by RNA sequencing (RNA-seq) in the presence or absence of Cin/Cit treatment. A total of 1713 differentially expressed genes (DEGs) were obtained, including 793 down-regulated and 920 up-regulated genes. Most of the DEGs participated in the biosynthesis of secondary metabolites, amino acid metabolism, and oxidation-reduction process, etc. Cin/Cit induced the dysfunction of the mitochondrial membrane, causing the potential influence on energy metabolism and reactive oxidative species production. The changes of superoxide dismutase (SOD) and catalase (CAT) activities combing with the increase of hydrogen peroxide content indicated the oxidative stress on P. expansum induced by Cin/Cit, which corresponded well with the transcriptional results. Moreover, both the RNA-seq data and the qRT-PCR showed the remarkable down-regulation of genes included in the PAT biosynthetic pathway under the Cin/Cit treatment. These findings provided more useful information about the antifungal mechanism of Cin/Cit against P. expansum at molecular and gene levels and suggested that Cin/Cit is a potential candidate to control P. expansum.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Phytopathogen Trichothecium roseum is one of the most significant spoilage fungi in China, causing decay in stored apples [1], melons [2,3], grapes [4], strawberries [5], and oranges [6]. Presently the pathogen can be controlled predominantly by chemical fungicides [7]. Nonetheless, decline in the number of available typical pink rot and preserved on potato dextrose agar (PDA) at 25 • C. The fungal conidia were washed from the surface of PDA plates with sterile 0.85% saline containing 0.1% Tween 80 (v/v) to prepare conidia inoculum and stored at 4 • C for further use.…”

Section: Introductionmentioning

confidence: 99%

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Zhang

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Trichothecium roseum is a harmful postharvest fungus causing serious damage, together with the secretion of insidious mycotoxins, on apples, melons, and other important fruits. Cuminal, a predominant component of Cuminum cyminum essential oil has proven to successfully inhibit the growth of T. roseum in vitro and in vivo. Electron microscopic observations revealed cuminal exposure impaired the fungal morphology and ultrastructure, particularly the plasmalemma. Transcriptome and proteome analysis was used to investigate the responses of T. roseum to exposure of cuminal. In total, 2825 differentially expressed transcripts (1516 up and 1309 down) and 225 differentially expressed proteins (90 up and 135 down) were determined. Overall, notable parts of these differentially expressed genes functionally belong to subcellular localities of the membrane system and cytosol, along with ribosomes, mitochondria and peroxisomes. According to the localization analysis and the biological annotation of these genes, carbohydrate and lipids metabolism, redox homeostasis, and asexual reproduction were among the most enriched gene ontology (GO) terms. Biological pathway enrichment analysis showed that lipids and amino acid degradation, ATP-binding cassette transporters, membrane reconstitution, mRNA surveillance pathway and peroxisome were elevated, whereas secondary metabolite biosynthesis, cell cycle, and glycolysis/gluconeogenesis were down regulated. Further integrated omics analysis showed that cuminal exposure first impaired the polarity of the cytoplasmic membrane and then triggered the reconstitution and dysfunction of fungal plasmalemma, resulting in handicapped nutrient procurement of the cells. Consequently, fungal cells showed starvation stress with limited carbohydrate metabolism, resulting a metabolic shift to catabolism of the cell's own components in response to the stress. Additionally, these predicaments brought about oxidative stress, which, in collaboration with the starvation, damaged certain critical organelles such as mitochondria. Such degeneration, accompanied by energy deficiency, suppressed the biosynthesis of essential proteins and inhibited fungal growth.fungicides to be used for postharvest treatment, reduced efficacy due to the emergence of pathogen resistance to these chemicals, and the growing public concerns over chemical residues in food and the environment have necessitated the seeking of alternative solutions to control the fungal spoilage and to guarantee food safety and consumer health [8].Essential oils (EOs), generally recognized as safe by the US Food and Drug Administration [9], are natural substances with established antimicrobial activities [10,11] and are a candidate instrument for controlling postharvest disease [12,13]. Nonetheless, this candidacy is compromised by the expensiveness of these natural substances due to their generally low yields. However, exploring their mode of actions is valuable to the development of novel alternatives.The multicomponent EOs attribute their mode of act...

show abstract

“…However, the use of these fungicides is becoming restricted owing to the increasing resistance of pathogens and a growing concern for the environment and human health. There have been numerous reports concerning novel and harmless antifungal agents, such as oxalic acid, clove oil, oligochitosan, boric acid, and potassium phosphate, amongst others, which can inhibit both the growth and patulin production of the postharvest pathogen P. expansum directly . A strong oxidant, ClO 2 is a Class A1 broad‐spectrum, highly efficient, safe chemical disinfectant recognized by the World Health Organization (WHO), as well as the Food and Agriculture Organization of the United Nations (FAO), which can be used for food preservation .…”

Section: Introductionmentioning

confidence: 99%

Effect of chlorine dioxide (ClO₂) on patulin produced by Penicillum expansum and involved mechanism

Zhang

Chen³

2018

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND Patulin, produced by Penicillium expansum in apple fruit, has side effects affecting human and animal health. The effect of chlorine dioxide (ClO2) on patulin production, and the mechanisms involved in this, were investigated. RESULTS Patulin production by P. expansum was reduced by ClO2 treatment, both in apples and in a potato dextrose broth (PDB) medium, which was attributed to the antifungal effect of ClO2, but not the direct reaction between ClO2 and patulin. Fumigation with ClO2 also significantly reduced disease development in apples infected with P. expansum, and inhibited mycelial growth and spore germination. After ClO2 treatment, P. expansum mycelial morphology was strongly affected, leading to the loss of plasma membrane integrity and causing cellular leakage. CONCLUSION These data provide useful information that enables the inhibitory mechanism of ClO2 on patulin production by P. expansum to be better understood. It can also assist the development of effective methods to control patulin production in apples and other postharvest fruits. © 2018 Society of Chemical Industry

show abstract

The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment

Cited by 32 publications

References 65 publications

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Effect of chlorine dioxide (ClO₂) on patulin produced by Penicillum expansum and involved mechanism

Contact Info

Product

Resources

About

The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment

Cited by 32 publications

References 65 publications

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

Effect of Cinnamaldehyde and Citral Combination on Transcriptional Profile, Growth, Oxidative Damage and Patulin Biosynthesis of Penicillium expansum

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Effect of chlorine dioxide (ClO2) on patulin produced by Penicillum expansum and involved mechanism

Contact Info

Product

Resources

About

Effect of chlorine dioxide (ClO₂) on patulin produced by Penicillum expansum and involved mechanism