The Resolution of &lt;i&gt;Helicobacter suis-&lt;/i&gt;associated Gastric Lesions after Eradication Therapy

Nakagawa, Satoru; Shimoyama, Tadashi; Nakamura, Masahiko; Chiba, Daisuke; Kikuchi, Hidezumi; Sawaya, Manabu; Chinda, Daisuke; Mikami, Taisei; Fukuda, Shinsaku

doi:10.2169/internalmedicine.8971-17

Cited by 13 publications

(22 citation statements)

References 22 publications

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“…In the present investigation, we did not use the data from the urea breath test (UBT), which is very sensitive and good for evaluations of the entire stomach. However, for the detection of NHPH both negative and weakly positive UBT results were reported, and it is sometimes difficult to apply UBT results to the detection of NHPH 4‐6 …”

Section: Discussionmentioning

confidence: 99%

“…We observed from the PCR data that every eradication regimen following the Hp treatment was quite effective. In some cases, clarithromycin was substituted to metronidazole because of the recent use of clarithromycin for the treatment of other diseases 6 . The endoscopic regression of the diseases was sometimes delayed, and this type of delay observed by the endoscopy and pathology in comparison with the bacteriological data is also seen during Hp eradication.…”

Section: Discussionmentioning

confidence: 99%

“…Although there are many reports of patients with various types of Helicobacter pylori (Hp)‐negative, non‐ Helicobacter pylori Helicobacter (NHPH) ‐ positive gastric diseases, 1‐6 the prevalence and the clinical significance of NHPH remain unknown. The prevalence rate has been reported to range from 0.25% in Germany, 0.36% in England, 0.3% in New Zealand, 0.6% in France, 7 and 1.2% in Czechoslovakia 8 to 1.9 to 11% in China, 9,10 2.7% in Turkey, 11 and 6.2% in Thailand 12 .…”

Section: Introductionmentioning

confidence: 99%

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PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Nakamura

Øverby²,

Michimae

et al. 2020

Helicobacter

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Background The clinical significance of non‐Helicobacter pylori Helicobacter (NHPH) is still unknown. There are many reports of NHPH‐infected patients suffering from gastric diseases. Here, we investigated the polymerase chain reaction (PCR) positivity of NHPH infection in gastric disease patients who were negative for H. pylori (Hp) by the rapid urease test and by pathological observation. Materials and methods We collected the 296 endoscopically obtained gastric mucosal samples of Hp‐negative gastric disease patients diagnosed based on a rapid urease test and pathology from 17 hospitals in Japan from September 2013 to June 2019, and we analyzed the existence of Hp and NHPH by PCR. The samples were also treated by indirect immunohistochemistry using an anti‐Helicobacter suis VacA paralog antibody and were observed by confocal laser microscopy. Results Among the 236 non‐Hp‐eradicated cases, 49 cases (20.8%) were positive for NHPH. Among them, 20 cases were positive for Helicobacter suis, 7 cases were positive for Helicobacter heilmannii sensu stricto/ Helicobacter ailurogastricus (Hhss/Ha), and the other 22 cases could not be identified. The regional differences in the infection rates were significant. Forty percent of the nodular gastritis cases, 24% of the MALT lymphoma, 17% of the chronic gastritis cases, and 33% of the gastroduodenal ulcer cases were NHPH positive. Forty‐five patients had been treated with one of the four types of combinations of a proton pump inhibitor and two antibiotics, and in all of these cases, the NHPH diagnosed by PCR was successfully eradicated. Immunohistochemistry using the Helicobacter suis‐specific HsvA antibody coincided well with the PCR results. Among the 29 post‐Hp eradication cases, three were NHPH positive, including one Hhss/Ha‐positive case. Thus, approx. 20% of the Hp‐negative non‐Hp‐eradicated gastric disease patients treated at 17 hospitals in Japan were infected with NHPH.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Nakamura

Øverby²,

Michimae

et al. 2020

Helicobacter

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“…In the literature, H. suis has been introduced as the most prevalent NHPGH species, colonizing the stomach of dyspeptic patients [13,27]. According to the recent study by Nakagawa et al, H. suis was the main cause of chronic gastritis in individuals without H. pylori infection [28,29]. According to previous studies, these species have a pathogenic potential due to the presence of gamma-glutamyl transpeptidase (ggt), their immune-suppressing properties, as well as outer membrane vesicles [13,30].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of non Helicobacter pylori gastric Helicobacters in Iranian dyspeptic patients

2020

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Background: Non Helicobacter pylori gastric Helicobacters (NHPGHs) are associated with a range of upper gastrointestinal symptoms, histologic and endoscopic findings. For the first time in Iran, we performed a crosssectional study in order to determine the prevalence of five species of NHPGHs in patients presenting with dyspepsia. Methods: The participants were divided into H. pylori-infected and NHPGH-infected groups, based on the rapid urease test, histological analysis of biopsies, and PCR assay of ureA, ureB, and ureAB genes. The study included 428 gastric biopsies form dyspeptic patients, who did not receive any treatment for H. pylori. The samples were collected and sent to the laboratory within two years. H. pylori was identified in 368 samples, which were excluded from the study. Finally, a total of 60 non-H. pylori samples were studied for NHPGH species. Results: The overall frequency of NHPGH species was 10 for H. suis (three duodenal ulcer, three gastritis, and four gastric ulcer samples), 10 for H. felis (one gastritis, three duodenal ulcer, and six gastric ulcer samples), 20 for H. salomonis (four duodenal ulcer, five gastritis, and 11 gastric ulcer samples), 13 for H. heilmannii (three gastritis, five duodenal ulcer, and five gastric ulcer samples), and 7 for H. bizzozeronii (zero gastric ulcer, two duodenal ulcer, and five gastritis samples). Conclusions: Given our evidence about the possibility of involvement of NHPGHs in patients suffering from gastritis and nonexistence of mixed H. pylori infection, bacteriological testing of subjects negative for H. pylori becomes clinically relevant and important. Our findings suggest H. salomonis has the highest rate among the NHPGH species in Iranian dyspeptic patients.

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“…The bacteria Helicobacter suis has been associated with gastric lesions in pigs and humans (DE BRUYNE et al, 2016;NAKAGAWA et al, 2018;RIMBARA et al, 2020), This leads to a major concern, since according to the data presented in the literature, people in close contact with pigs are exposed to higher risks of infection, suggesting that this animal is a zoonotic agent (JOOSTEN et al, 2013;LOUGHLIN et al, 2019). Helicobacter suis is one of the most prevalent non-H. pylori sp.…”

Section: Microbiologymentioning

confidence: 99%

Helicobacter spp. and gastric lesions association in minipigs

et al. 2021

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Due to their similarity to humans, studies regarding gastric ulcers in pigs have become of great interest. It is known that Helicobacter spp. is related to the occurrence of these ulcers, as they possess a high prevalence in pigs. This study aimed to associate gastric lesions findings naturally occurring in minipigs related to or not related to the presence of Helicobacter spp., through the ultra-rapid urease test and immunohistochemical analysis, reaffirming the disease in swine as a natural biomedical model for human cases. For this, samples of formalin-fixed paraffin-embedded gastric tissues from 40 minipigs were obtained at the Laboratório de Morfologia e Patologia Animal (LMPA) in the Universidade Estadual do Norte Fluminense (UENF). Regarding the ultra-rapid urease test, no animal was positive in all regions. However, 18 were positive in at least one: six (15%) were positive in the aglandular region, eight (20%) in the antrum region, 13 (32.5%) in the region of the cardiac gland, and one (2.5%) in the region of the fundic gland. Regarding immunohistochemical analysis, only one animal was positive in all regions, and 32 animals were positive in at least one: four (10%) were positive in the aglandular region, 10 (25%) in the antrum region, 23 (57.5%) in the region of the cardiac gland, and three (7.5%) in the region of the fundic gland. The gastric lesion findings showed a close relationship with Helicobacter spp., enriching the laboratory animal pathologies list. The immunostaining of the bacteria not associated with gastric lesions in certain regions demonstrated the saprophytic and opportunisitic nature of Helicobacter.

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The Resolution of <i>Helicobacter suis-</i>associated Gastric Lesions after Eradication Therapy

Cited by 13 publications

References 22 publications

PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Prevalence of non Helicobacter pylori gastric Helicobacters in Iranian dyspeptic patients

Helicobacter spp. and gastric lesions association in minipigs

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