The resistance of sangkuriang catfish (Clarias gariepinus Burchell 1822) against Aeromonas hydrophila bacteria given moringa leaf extracts (Moringa oleifera L.) through the feed

Rosidah,; Buwono, Ibnu Dwi; Lili, Walim; Suryadi, Ibnu Bangkit; Triandika, Ade Reza

doi:10.32491/jii.v19i1.435

Cited by 10 publications

(10 citation statements)

References 4 publications

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“…There is an influence from other factors in increasing the number of leukocytes, one of which is the addition of chitosan to the feed. Chitosan is able to induce an increase in the number of white blood cells with immunodulation activity that optimizes the body's immune function, this is in line with the statement of Rosidah et al, (2019), namely increasing the number of white blood cells in fish given Moringa leaf extract which has immunomodulatory activity which plays a role in making the immune system more active in carrying out its function in strengthening the body's immune system.…”

Section: White Blood Cells (Leukocytes)supporting

confidence: 78%

“…Clinical symptoms began to appear 24 hours after infection, in the form of a distended abdomen on the body (Figure 10 d) that occurred in catfish fry in treatment A, while in other treatments there were no clinical symptoms. As according to Rosidah et al (2019), 24 hours after infection by the bacterium A. hydrophila, fish suffered damage to their bodies in the form of inflammation (inflammation), bleeding (hemorrhagic) and ulcers (ulcer). The occurrence of ulcers in some fish is thought to be due to the high density of bacteria in the area, so that the intensity of toxins released by bacteria is also high.…”

Section: Clinical Symptomsmentioning

confidence: 99%

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The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack

Radityo

Rosidah

Lili

et al. 2022

Jurnal Akuakultur Indonesia

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This research aims to determine the optimal dose of chitosan that is appropriate to be given to commercial feed in increasing the immune system of sangkuriang catfish fry. The research was carried out in April – June 2021 at the Aquaculture Laboratory and Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research used an experimental method of Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the addition of chitosan to feed with doses of 0 g (A), 2.5 g (B), 5 g (C), 7.5 g (D), and 10 g (E). Maintenance of test fish for 14 days. Experimental fish were infected by Aeromonas hydrophila bacteria with a density of 108 CFU/ml with 10 ml immersion method. Parameters observed included white blood cells, leukocyte differential, clinical symptoms, survival and water quality. Data on white blood cell count, and survival were analyzed by ANOVA, if there was a significant difference, Duncan's test was performed. Leukocyte differential data, clinical symptoms and water quality were analyzed descriptively. The results showed that the addition of chitosan at a dose of 10 g was effective for improving the immune system performance of catfish fry. During 14 days of chitosan administration, the white blood cell count increased by 41.60% from the value of 13.96 × 104 cells/mm3 to 23.90 × 104 cells/mm3. The 10 g chitosan treatment was an effective dose to stimulate differential leukocytes in catfish to improve the immune system, it was also marked by the fastest clinical symptom recovery period and the highest survival rate after the challenge test, which was 96.6%.

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Section: White Blood Cells (Leukocytes)supporting

confidence: 78%

Section: Clinical Symptomsmentioning

confidence: 99%

The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack

Radityo

Rosidah

Lili

et al. 2022

Jurnal Akuakultur Indonesia

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“…Feeding was carried out for 14 days (Valenta et al, 2018). The frequency of feeding was carried out three times a day at 08.00 am, 11.00 am, and 16.00 pm (Rosidah et al, 2019).…”

Section: Feed Compositionmentioning

confidence: 99%

Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack

Silviana

Rosidah

Pamungkas

et al. 2022

Jurnal Akuakultur Indonesia

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ABSTRACT This study aims to determine dose of black cumin flour in fish feed diets which can improve the disease resistance of tilapia against the attack of A. hydrophila. This study was used tilapia juvenile with a size of 7 – 8 cm. This study was conducted from March – May 2021 and the experimental method is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used were addition of black cumin flour into fish feed with doses 0 (A), 20 (B), 35 (C), 50 (D), and 65 (E) g/Kg feed. The treatments fed by supplemental diets contained black cumin flour was conducted for 14 days, then a challenge test was carried out with the infection of A. hydrophila bacteria by immersion for14 days. A. hydrophila bacteria used to infect fish had a density of 108 CFU/mL. The Parameters used were total leukocyte, hematocrit, total leukocyte differential, clinical symptoms, survival rate and water quality. The result showed that black cumin flour was effective to increase the immunity system of tilapia. The dose of 50 g/Kg feed was effective showed increase the highest percentage increase in white blood cells of 64.56%, compared to treatments B, C, and E which showed an increase in white blood cells of 47.40%, 48.82% and 1.2%, respectively. Treatment A decreased the percentage of white blood cells by 19.52%. The 50 g dose of black cumin showed the highest percentage value of hematocrit and leukocyte differential in tilapia fry, respectively 34%, lymphocytes 84.3%, monocytes 6%, and neutrophils 9.7% with the fastest recovery period (4 days) and after the challenge test resulted in the highest survival rate of 95%. Keywords: Aeromonas hydrophila, tilapia juvenile, black cumin, immune system ABSTRAK Penelitian ini bertujuan untuk menentukan dosis tepung jintan hitam dalam formula pakan ikan yang dapat meningkatkan ketahanan tubuh ikan nila terhadap serangan bakteri A. hydrophila. Penelitian ini menggunakan benih ikan nila dengan ukuran 7-8 cm. Penelitian ini dilaksanakan pada bulan Maret – Mei 2021 dan metode eksperimen yang digunakan adalah model Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan tepung jintan hitam pada pakan dengan dosis 0 (A), 20 (B), 35 (C) 50 (D) dan 65 (E) g/Kg pakan. Pemeliharaan ikan uji yang diberikan perlakuan yang mengandung tepung jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantang menggunakan bakteri A. hydrophila dengan metode immersi selama 14 hari. Bakteri A. hydrophila yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL. Parameter yang digunakan yaitu leukosit, hematokrit, differensial leukosit, gejala klinis, kelangsungan hidup dan kualitas air. Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan ketahanan tubuh ikan nila. Dosis jintan hitam 50 g per 1 kg pakan paling efektif, terlihat dari persentase peningkatan sel darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, dan E yang masing masing menunjukan peningkatan sel darah putih sebesar 47.40%, 48.82% dan 1.2%. Perlakuan A mengalami penurunan persentase sel darah putih sebesar 19.52%. Dosis jintan hitam 50 g menunjukkan nilai persentase hematokrit dan differensial leukosit yang paling tinggi pada benih ikan nila, masing-masing sebesar 34%, limfosit 84.3%, monosit 6%, dan neutrofil 9.7% dengan masa pemulihan tercepat (4 hari) dan setelah di uji tantang menghasilkan kelangsungan hidup tertinggi yaitu sebesar 95%. Kata kunci: Aeromonas hydrophila, benih ikan nila, jintan hitam, sistem imu

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“…Sangkuriang catfish (Clarias gariepinus) is a freshwater aquaculture commodity that is widely and intensively cultivated in Indonesia. They are known to be resistant to disease and have rapid growth potentials [17]. Furthermore, the protein content in catfish feed is generally about 32-36%, with the majority originating from fish meal [18].…”

Section: Introductionmentioning

confidence: 99%

Growth and Feed Digestibility of Sangkuriang Catfish with Corn Gluten Meal-Based Feeding

Mustahal

Hermawan

Syamsunarno

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Diet testing was conducted for 50 days to investigate the growth and feed digestibility of Sangkuriang catfish with corn gluten meal (CGM)-based feeding. This involved 5 treatments with different levels of CGM in the feed 0, 75, 150, 225, and 300 g.kg−1 for three replications. The result showed that the value of SGR ranged from 0.87 to 1.51 %.day−1, the SGR values with CGM at 0, 75 and 150 g.kg−1 were significantly higher than the values in CGM 225 and 300 g.kg−1. The best value of protein and lipid digestibility were recorded with CGM at 0, 75 and 150 g.kg−1 compared to CGM at 225 and 300 g/kg. A greater propensity for higher CGM levels to negatively impact on growth and feed digestibility of Sangkuriang catfish. Therefore, CGM is confirmed to process the potential for application at a level of 150 g.kg−1 in the Sakuriang catfish diet without negatively influencing growth and feed digestibility.

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The resistance of sangkuriang catfish (Clarias gariepinus Burchell 1822) against Aeromonas hydrophila bacteria given moringa leaf extracts (Moringa oleifera L.) through the feed

Cited by 10 publications

References 4 publications

The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack

The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack

Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack

Growth and Feed Digestibility of Sangkuriang Catfish with Corn Gluten Meal-Based Feeding

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