The Renal Excretion of Bilirubin in Dogs with Obstructive Jaundice*

Dialyzability, Protein Binding, and Renal Excretion of Plasma Conjugated Bilirubin*

“…The findings in our earlier renal function experiments in jaundiced dogs were consistent with a predominant process of glomerular filtration of conjugated bilirubin (9). Collodion sac ultrafiltrations of jaundiced plasma failed to yield ultrafilterable bilirubin in this laboratory.…”

“…9 The precise proportion was difficult to define because of the methods of dialysis and of bilirubin estimation. The typical absorption spectra of the native pale yellow dialyzates and of their violet azo derivatives suggested that the dialyzable plasma pigment was bilirubin.…”

“…Jaundice was induced in the dogs by common bile duct ligation, and renal function studies were performed as described previously (9). In two dogs the right ureter was ligated at the time of the common bile duct operation, and the left ureter was operatively ligated about 10 days later.…”

“…Serum urea nitrogen levels were estimated on an autoanalyzer.1 Hexuronic acid was determined by the method of Dische (13). The other chemical methods were described previously (9).…”

“…Studies that were performed in jaundiced dogs in this laboratory gave results consistent with a process of glomerular filtration of conjugated bilirubin (9). We therefore re-examined the question of the dialyzability of plasma bilirubin.…”