The relationships between HDL mean particle size and serum paraoxonase activity

Razavi, Amir Nader Emami; Ani, Mohsen; Naderi, Gholam Ali; Pourfarzam, Morteza

doi:10.1016/j.clinbiochem.2011.08.101

Cited by 20 publications

(22 citation statements)

References 45 publications

(58 reference statements)

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“…Previous studies have postulated that the capacity of HDL particles to carry PON1 depends on their structure . Our results clearly demonstrated that PON1 activity remained unaltered among the study groups, in spite of the structural modifications exhibited by HDLs.…”

Section: Discussionsupporting

confidence: 67%

Atorvastatin and fenofibrate combination induces the predominance of the large HDL subclasses and increased apo AI fractional catabolic rates in New Zealand white rabbits with exogenous hypercholesterolemia

Flores-Castillo

Zamora-Pérez

Carreón‐Torres

et al. 2015

Fundamemntal Clinical Pharma

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The anti-atherogenic properties of high-density lipoproteins (HDLs) may be related to their structure and metabolism. The HDL physicochemical characteristics that determine their plasma clearance during treatment with statins and fibrates are not well understood. In this study, we analyzed HDL-apo AI fractional catabolic rates (FCRs), size distributions, and the lipid composition of the HDL subclasses in New Zealand white rabbits with exogenous dyslipidemia that received low doses of atorvastatin and fenofibrate. Hypercholesterolemia decreased only partially with the combination of both drugs. HDL size distribution shifted toward larger particles among the groups of rabbits that received atorvastatin, fenofibrate, or their combination, compared with both the control group and the dyslipidemic group. The HDL subclasses were significantly rich in cholesterol in each of the groups compared with controls. The structural changes noted in the HDL subclasses were not associated with impaired plasma paraoxonase-1 (PON1) activity. The groups receiving monotherapy and the drug combination group were all associated with a higher apo AI FCR value compared with both the dyslipidemic rabbits and the control group. In conclusion, the combination of atorvastatin and fenofibrate induced a more favorable HDL subclass profile than did the individual use of these drugs. Similarly, the apo AI FCR values were augmented in every group receiving drug treatment (either monotherapy or combination therapy) in the setting of hypercholesterolemia. The anti-atherogenic properties of HDLs, excluding their capacity to bind PON1, may be enhanced by the structural and metabolic modifications induced by the combination of atorvastatin and fenofibrate.

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Section: Discussionsupporting

confidence: 67%

Atorvastatin and fenofibrate combination induces the predominance of the large HDL subclasses and increased apo AI fractional catabolic rates in New Zealand white rabbits with exogenous hypercholesterolemia

Flores-Castillo

Zamora-Pérez

Carreón‐Torres

et al. 2015

Fundamemntal Clinical Pharma

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“…Similarly, a recent epidemiologic study of 104 healthy volunteers reported a negative association between HDL particle size and PON1 POase activity, and a negative correlation between HDL2:HDL3 ratio and PON1 POase activity [63]. Taken together, these results suggest that PON1 is strongly associated with apoAI, and that they are found more frequently associated with the small, dense subfractions of HDL.…”

Section: Paraoxonases and Human Diseasementioning

confidence: 53%

Pharmacogenetics of Paraoxonase Activity: Elucidating the Role of High-Density Lipoprotein in Disease

et al. 2013

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PON1 is a key component of high-density lipoproteins (HDLs) and is at least partially responsible for HDL's antioxidant/atheroprotective properties. PON1 is also associated with numerous human diseases, including cardiovascular disease, Parkinson's disease and cancer. In addition, PON1 metabolizes a broad variety of substrates, including toxic organophosphorous compounds, statin adducts, glucocorticoids, the likely atherogenic l-homocysteine thiolactone and the quorum-sensing factor of Pseudomonas aeruginosa. Numerous cardiovascular and antidiabetic pharmacologic agents, dietary macronutrients, lifestyle factors and antioxidant supplements affect PON1 expression and enzyme activity levels. Owing to the importance of PON1 to HDL function and its individual association with diverse human diseases, pharmacogenomic interactions between PON1 and the various factors that alter its expression and activity may represent an important therapeutic target for future investigation.

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“…Serum PON1 activity was assayed using 2 mM paraoxon (diethyl p-nitro-phenyl phosphate, Sigma Chemical Co., St. Louis, Mo, USA) as the substrate in 100 mM tris buffer (pH 8.0) containing 2 mM of CaCl 2 [20]. A 40 μ L aliquot of serum was added to 500 μ L of substrate medium, and the initial rate of hydrolysis (generation of p-nitrophenol) was monitored at 412 nm, using a continuously recording spectrophotometer (UV 3100, Shimadzu, Kyoto, Japan) over a period of 2 min.…”

Section: Methodsmentioning

confidence: 99%

Serum Paraoxonase 1 Activity Is Associated with Fatty Acid Composition of High Density Lipoprotein

et al. 2013

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Introduction. Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). Fatty acid composition of HDL could affect its size and structure. On the other hand, PON1 activity is directly related to the structure of HDL. This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men. Methods. One hundred and forty healthy men participated in this research. HDL was separated by sequential ultracentrifugation, and its fatty acid composition was analyzed by gas chromatography. PON1 activity was measured spectrophotometrically using paraxon as substrate. Results. Serum PON1 activity was directly correlated with the amount of stearic acid and dihomo-gamma-linolenic acid (DGLA). PON1/HDL-C was directly correlated with the amount of miristic acid, stearic acid, and DGLA and was inversely correlated with total amount of ω6 fatty acids of HDL. Conclusion. The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. As dietary fats are the major determinants of serum lipids and lipoprotein composition, consuming some special dietary fatty acids may improve the activity of PON1 and thereby have beneficial effects on health.

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The relationships between HDL mean particle size and serum paraoxonase activity

Cited by 20 publications

References 45 publications

Atorvastatin and fenofibrate combination induces the predominance of the large HDL subclasses and increased apo AI fractional catabolic rates in New Zealand white rabbits with exogenous hypercholesterolemia

Atorvastatin and fenofibrate combination induces the predominance of the large HDL subclasses and increased apo AI fractional catabolic rates in New Zealand white rabbits with exogenous hypercholesterolemia

Pharmacogenetics of Paraoxonase Activity: Elucidating the Role of High-Density Lipoprotein in Disease

Serum Paraoxonase 1 Activity Is Associated with Fatty Acid Composition of High Density Lipoprotein

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